2. REVIEW OF LITERATURE
2.1. Biogenic Amines:
2.1.1. Origin of biogenic amines:
Pipek et al. (1992) recorded that biogenic amines are compounds commonly present in living organisms in which they are responsible for many essential functions. They are present in low concentrations in human, animals and plants for regulating functions, acting on the nervous system. Therefore, biogenic amines are naturally found in many foods including meat and can be produced in high amounts by microorganisms through the activity of amino acid dccarboxylases. They can also formed by autolytic proteolysis of meat enzymes. Histamine is formed from histidine, cadaverinc from lysine and putrescine from ornithin.
Davidek and Davidek (1995) stated that biogenic amines are normally formed in food as a consequence of the metabolic process during storage, spoilage or ripening. After degradation of proteins either by autolysis or by bacterial proteolysis, free amino acids are formed which act as precursors of biogenic amine with the aid of decarboxylase enzymes.
Straub et al. (1995) mentioned that the biogenic amines in foods are mainly formed by amino acid decarboxylation of bacteria. During a fermentation process the protein breakdown products, peptides
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Histamine, putrescine, cadaverine, tyramine, tryptamine, β phenylethylamine, spermine and spermidine are considered to be the most important biogenic amines occurring in foods. The factors which influence the formation of biogenic amines in foods include the availability of free amino acids; the presence of micro-organisms that can decarboxylate these amino acids and the favorable conditions of such micro-organisms for the growth and production of their
Each amino acid is made up of an amino group, a carboxyl group and a side chain (Reece, J. B., Urry, L. (2016). Campbell biology. Boston Pearson). Enzymes work by lowering the activation energy of the reaction making the reaction produce faster. Enzymes begin to catalyze chemical reactions with the binding of the substrate to the active site on the enzyme.
11. P-Phenylenediamine P-Phenylenediamine, a coal tar dye, is found in numerous hair dyes and other cosmetics. But, it is especially found in darker hair dyes. Coal tar dyes, including P-Phenylenediamine, are a mixture of many different chemicals. They come from petroleum and are known as a human carcinogen.
A: Isolate HHHHHHH Method: Ni-Affinity Chromatography Reason: Six histadine amino acids at the end of the protein can bind to nickel very tightly. Nickel can bind to agarose bead very tightly. Use this strong affinity column we can isolate our protein, which has seven histadine amino acids. Buffer condition: His-binding buffer: • 50 mM Tris-HCl (pH8.0) • 5 mM Imidazole • 100 mM NaCl • 0.1 mM EDTA • 1 mM PMSF made fresh His-wash buffer: • 50 mM Tris-HCl (pH8.0) • 300 mM NaCl • 15 mM Imidazole • 0.1 mM EDTA • 1 mM PMSF made fresh is-elution buffer: • 50 mM Tris-Cl (pH8.0) • 50 mM NaCl • 300 mM Imidazole • 0.1 mM EDTA • 1 mM PMSF made fresh Procedure: 1.
This is a choice between providing consumers with pesticide-free food products versus pesticide-laden processed food products. Industrial food production started in 1947 when the chemical industry decided to shift their focus away from war-time technology to more peaceful applications, one of which was creating chemical fertilizers to help farmers in raising their crops. It was advertised that the synthetic fertilizers would allow farmers increase the fertility of their soil without the need
This lab was designed to study the generation of β-Galactosidase over a 2 lab period, so it got 2 sections; first part was to measure the levels β-galactosidase produced in E.coli K12 cells specifically using IPTG a molecular biology reagent to determine the time of induction of the lac operon. The second part of this experiment was to observe the effects of alternative inducing agents, glucose and antibiotic addition on the induction of β-galactosidase in E.coli K12; this experiments goal was to detect the effect of alternate induction agents, antibiotic and glucose adding on to inducing of β-galactosidase in E.coli. The β-galactosidase is normally switched off in E.coli except in the presence of lactose; the enzyme β-galactosidase breaks down lactose into galactose and glucose. (Matthews 2005). The lac operon or lactose operon is essential for the transportation of lactose in E.coli.
Chapter 2 of The poisoner’s handbook by Deborah Blum has an aggressive style. Elements of diction support this style. Some of the elements that support the style are connotation/ denotation, cacophonous, and monosyllabic/polysyllabism. Connotation and denotation help to show the aggressive style of the writing in chapter 2. When describing mustard gas, Blum states the its effect include “searing the eyes into a crusted blindness” (Blum, 2010).
L-Norvaline is an analog of the branched chain amino acid, valine. It can be found in foods such as dairy, meat, grains, soy and peanuts. L- Norvaline can only be produced through a diet because the body doesn’t produce it. L-Norvaline also increases the nitric oxide levels in the blood which can lower blood pressure and help improve the overall health of the user. TAURINE
Tryptophan is an essential amino acid, which is either synthesized or injested from the environment in bacterial cells. The production of tryptophan is an energy consuming process. So bacteria need to ensure that only when the concentration of tryptophan is low inside cell , it has to produce tryptophan. The trp operon is a repressible system that regulates the biosynthesis of tryptophan. The gene for the repressor is located not near to the promoter,but in a different location.
1. Write a sentence for each of these mechanisms describing the manner in which the DNA can be transferred from one cell to another. Transformation: During transformation pieces of genetic instructions are released by a bacterium. Another bacterium, picks up the DNA into its own genome. Bacteria taking up foreign DNA is known as transformation. Transformation implies uptake in bacterial, yeast or plant cell DNA while transfection is the term used in reference of mammalian uptake.
The Rmaz and EC50 of histamine control were 16.49gms and 2.093 x 10-7M respectively. In figure 1, when 5 x 10-9M, 5 x10-8M and 5 x 10-7M of mepyramine were added, the EC50 of histamine was increased to 6.254 x 10-6M, 4.752 x 10-5M and 0.0002428M respectively. The Rmaz of histamine in the presence of final bath concentration (FBC) of 5 x 10-9M, 5 x10-8M and 5 x 10-7M antagonist either mepyramine or drug A were remained almost constant in Figure 1 and 2. On the other hand, the EC50 of histamine was increased to 3.455 x10-5M, 0.0001693M and 0.0006647M during the presence of FBC of 5 x 10-9M, 5 x10-8M and 5 x 10-7M drug A respectively. Hence, higher histamine’s concentrations were needed to achieve EC50 as the antagonist’s concentration increased.
Throughout the urea cycle, the amino acid, arginine, is changes into ornithine- this is another amino acid when hydrated, that is when water was added. During this reaction, urea is the product formed (Nelson and Cox 2008). Figure 1 shows the urea cycle, occurs specifically in the mitochondria and cytosol in the liver. (Nelson and M.Cox 2008). Urea is made in the liver by means of enzymes in the urea cycle.
Agitation rpm level tannase production Agitation rate was at a range of 50 to 250 rpm level was chosen to determine the optimal rate. It was found that an agitation rate of 100 rpm at pH 6.0 and 37°C maximum yield of tannase production of 3.12 U/ml (Fig.4) it was also noted that an increase in agitation speed above100 rpm resulted in a drastic fall in tannase enzyme production. The agitation speed below 100 rpm level resulted in an inadequate mixing of the broth towards of the broth. Towards the later stages of growth. Effect of carbon and nitrogen sources
This helps lipase an enzyme from pancreas hydrolyzed triglycerol in to free fatty acids and monoglycerides. The purpose of this experiment is to synthesize digestion of carbohydrates, lipids and peptides using enzymes that catabolize them in order to further understand all the components that goes need to be meet for digdestion to happen by manipulating different variables like pH, and
Zinc is thought to be anti-cancerous and to prevent pre-mature ageing. Zinc’s main role is in the protection of DNA and it plays a major role in nearly every disease from cancer to diabetes as it is needed to make insulin, to boost the immune system and to make an enzyme called SOD (super oxide dismutase) which is a very important antioxidant, which helps disarm free radicals. There is however a danger regarding intake of too much zinc. It is recommended to take 25-50mg of a zinc supplement per day. It increases your libido and your vitality.
Methylation is a product of complex processes that move and transform mercury. Atmospheric deposition contains the three principal forms of mercury, although inorganic divalent mercury (HgII) is the dominant form. Once in surface water, mercury enters a complex cycle in which one form can be converted to another. Mercury attached to particles can settle onto the sediments where it can diffuse into the water column, be resuspended, be buried by other sediments, or be methylated. Methylmercury can enter the food chain, or it can be released back to the atmosphere by volatilization.