Introduction Medical instruments are routinely sterilized using autoclave or by gas sterilization using ethylene oxide. However this method of sterilization is insufficient to destroy pyrogen because it is thermostable as a matter of fact, pyrogens will be formed from the dead gram-negative bacteria that is killed during the sterilization process. The pyrogen substance that is produces is called endotoxin. Medical instruments (such as surgical instruments and in certain cases of implants) may contain endotoxin on the surface of the instruments, due to this the LAL test is performed (Mazzotti et al, 2007). Endotoxin is released into the environment during bacterial cell death or cell division occurs. Endotoxin is known as a potent inflammatory …show more content…
Then the blower was turned on for sufficient duration and UV lights were switched on for one hour. LAF microbial contamination test (Settling plate method)
A total of three petri dishes were prepared aseptically inside a laminar air flow (LAF), and then the petri dish was filled by pouring sterile Tryptone Soy Agar (TSA) liquid media and allowed to solidify. One test media was placed in the LAF cabinet, one test media is placed beside the LAF cabinet while the other the test media was placed near the door. The three petri dishes lid were opened and allowed to stand for 15 minutes and closed again. Then, the test media is then incubated at 37 ° C, for 18-24 hours.
Rinsing reusable instruments
The samples were rinsed with 40 ml of pyrogen-free water using a glass beaker that is free from pyrogens.
Endotoxin testing using STV
A total of 0,2mL from the water obtained from the rinsing was placed in the STV containing LAL reagent and was shaken for 20 to 30 seconds. Then STV was placed in an incubator at 37 ° C for 60 ± 2 minutes. STV was then observed by reversing the reaction tube in one smooth motion. Negative controls performed using pyrogen-free water that is poured into a glass beaker which has been
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This is shown by the negative result by the absence of microbial growth on the growth media Tryptic Soy Agar (TSA), the LAF cabinet meets the requirements according to Good Manufacturing Practice (GMP) sterile room, where the number of bacteria colonies in the LAF cabinet must be below 1 cfu/m3 which is a requirement for a class A area (C.Pasquarella, O.Pitzurra, & A.Savino, 2000).
The LAF area is a class I area or it is also known as a white area. This area must fulfill the requirements regarding the amount of microbes and particles. LAF room equipped with a hepafilter that filters the air flowing in the cabinet, the air movement in the LAF cabinet is laminar so that the microbes as well as particle from outside the room cannot enter into the area (Rees, Smith, & Watson, 2014).
Results of rinsed reused sterile medical instruments.
As much as 40ml of pyrogen free water used to rinse the reusable medical instruments, the amount of pyrogen free water which was used is done in accordance with the formula for endotoxin limits on rinsing. The formula which is used is as below (USP,
After 5 days the plates were removed from the cold room and the gram-negative test for Colony A on the EMB agar showed pink fisheye colonies which lead to the conclusion that the gram-negative organism within Unknown #21 was Enterobacter aerogenes. Had the pigmentation been metallic green, the organism would have been identified as Escherichia coli, and had there been no pigmentation at all a Triple Sugar Iron agar (TSI) test among other tests would have been
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
The floor scrubbers are not currently sampled for bacteria in the tank, brushes and wheels. They move all through defined areas but were observed to be dirty. " Verification of
In addition, I also cleaned the handrail in the Elementary building, and these results were quite interesting. Before I cleaned the surface there was only 2.3% bacteria. Which, compared to my other results
Tools to be sterilized regularly using barbicide and the use of the UV
It is necessary to eliminate the possibility of contamination of the starting materials and/or products of other materials and products. Increased risk of accidental cross-contamination contributes to the uncontrolled spread of dust, gases, vapours, aerosols or micro-organisms present in the materials and products, equipment, and clothing (Coyle, et.al, 2004, 179-186). The degree of risk depends on the type of contamination and product exposed to contamination. The most dangerous pollutants are sensitizing substances, biological preparations containing living organisms, certain hormones, cytotoxics and potent substances (Pye & Blott, 2009, 170-181). Contamination is particularly dangerous for drugs used for injection or for the treatment of open wounds and also intended for prolonged use and/or receiving high doses.
Employees working in pediatrics hospital are supposed to take precautions to protect their patients and staff from exposure to potentially infectious materials. A fundamental component of standard infection prevention measures are a system of barrier precautions to be used by all personnel
shall be guided by the following principles: 1. Medical devices, appliances and articles used in surgery shall be sterilized. 2. Disposable medical devices and apparatus shall comply with the relevant state regulations and shall not be reused.
Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
Astronauts will swab surfaces within the cabin, mix swabbed material in liquid form to the LOCAD-PTS, and obtain results within 15 minutes on a display screen. The study 's purpose is to effectively provide a rapid
This extra barrier of protection is important because cleaning with soap and water is insufficient and will only remove visible dirt and only partial germs. One of the important duties of the vet nurse/ assistants is to keep a high level of hygiene within the surgery; you should always follow a clean to dirty rule when cleaning and clean/tidy up as you go. All sinks should be cleaned thoroughly each morning and clean cloths/towels provided, Floors of the consulting rooms and waiting room should be thoroughly swept and mopped before and after each surgery to minimise infections, examination tables should be sprayed with an anti-bacterial solution between patients, Kennels should be cleaned and sprayed with disinfectants after every patient, bedding should be shaken free of dust and hair before washing at the highest temperature in the washing machine in order to destroy any harmful pathogens, Items that have be heavily contaminated should be discarded. Nurses/assistants are also responsible for cleaning medical kits and drapes, these not only need to be cleaned thoroughly to remove any blood and tissue debris but also require to be sterilised using an Autoclave, this is the most common method of sterilizing kits ( Autoclaving is the process used to destroy all micro-organisms and infectious bio waste. Autoclaving promotes
As mentioned previously, biohazardous wastes can cause critical viruses in humans or diseases. Without things being done properly, missing the simplest step, could be more than dangerous. Just as the possibility of bacterial growth, affects more than just those in the room of the lab. Exposure to unwanted chemicals is a major risk, and keeping up with the guidelines is more than important. To have the best outcome, without minor mistakes, and no chance of health risks, following a safety checklist can be helpful.
Research question: Does green tea inhibit oral bacteria? Aim: To determine whether green tea inhibits oral bacteria Hypothesis: The strongest green tea solution will inhibit the greatest amount of growth in oral bacteria Independent variable:
According Association of perioperative Registered Nurses (AORN) standard, healthcare facilities that reprocessing medical devices must have quality control program. For example, monitor steam sterilization cycles using physical, chemical and biological indicators, having a product recall procedures, maintaining appropriate documentation and reporting system that enable traceability to each sterilized medical items to patient ( AORN). In author’s hospital, there are three table top flash sterilizers in operating theatre. Perioperative nurses are responsible to process IUSS including decontamination of the instruments. To ensure the IUSS meet the AORN standard, the charge nurse will carry out daily cleaning of the table top flash sterilizer.
- The contaminated personal protective equipment should be discarded in appropriate disposal containers, and get red them as the policy of the hospital. - Sharing personal protective equipment on dealing with patients should be avoided. - Personal protective equipment should be changed completely and hands should be washed after leaving a patient to care another patient or to do another duty.