The first test that gave us an indication that catharant hus ovalis (species Z), is most closely related to Catharanthus roseus (Rosy periwinkle) is test 5 (Test for enzyme M). We found that both species Z and rosy periwinkle have enzyme m present which suggests that share similar enzymes, which helps prove that species Z can produce the same alkaloids. Enzymes are used to increase reaction and help with digestion/ synthesis. Enzyme m, which is present in periwinkle, is used to synthesize the alkaloids of interest. We tested the 3 other species to see if this enzyme was present to help bring us to our conclusion of which is most similar to rosy periwinkle. To perform this lab, we had a tray with 4 smaller scoops of indicator power (one for each species) and added one drop of plant extract to the correctly labeled powder. …show more content…
Rosy periwinkle contains enzyme m, so whichever species fizzed means they do as well. Both species z and evening primrose (species y) fizzed up but, species z had more common traits with periwinkle then species y did, which is why we believe its species Z and not Y. Enzyme m synthesizes alkaloids which is shown in both rosy periwinkle and species Z. This evidence indicates to us that because rosy periwinkle and species Z have similar enzymes, they both also share similar DNA which proves they both must produce alkaloids. Enzymes code for many qualities in a flower, its job is to catalyze a biochemical reaction and makes the rearrangement of a molecules chemical group easier. In this case, enzyme m helps synthesize alkaloids of interest. This helps us bring us to our conclusion that they must have the same DNA because they have the same enzyme that codes for the same job in synthesizing alkaloids. The experiment we conduced included having 4 different indicator powders for each species; followed by adding 1 drop of plant extract to each
Name: Avishak Deb Roy Partners: Leevell Penn, Varugh, Butler Bio 101 Lab Report #1 02.22.2018 Swimming speed of paramecium tetraurelia in different levels of treatment. Introduction Paramecia is a unicellular Protista which are naturally found in aquatic habitats. It is easily cultured in the laboratory. It is oblong shaped and covered with short hairy structure called cilia. Paramecia does not pose any health or ethical concerns and the population can be maintained if there is a food source such as Enterobacter (Biological Foundation 7).
ST Report In the experiment, the problem was the contaminants that were affecting the quality of the water samples. To fix this issue, three scientists had to determine the contaminants that were present in the samples. One sample was from the school sink and the second sample was from an unknown source. The scientists conducted many tests to figure out what pollutants were present in the water.
Introduction: Enzymes are needed for survival in any living system and they control cellular reactions. Enzymes speed up chemical reactions by lowering the energy needed for molecules to begin reacting with each other. They do this by forming an enzyme-substrate complex that reduces energy that is required for a specific reaction to occur. Enzymes determine their functions by their shape and structure. Enzymes are made of amino acids, it 's made of anywhere from a hundred to a million amino acids, each they are bonded to other chemical bonds.
The purpose of this experiment was to analyze the effects of the variables: temperature, pH, and enzyme concentration, on the enzymatic reaction rate of catalase and the level at which its products are released, measuring the rate of absorption using the indicator solution guaiacol and a spectrophotometer to develop a hypothesis of the ideal conditions for these reactions. My hypothesis is that the extremes in concentration, temperature and pH will negatively affect the Au rate. This experiment used 11 solutions contained in cuvettes. Each cuvette, once mixed, is placed in spectrophotometer and then a reading taken every 20 seconds. Cuvettes 1, 8, and 10 are used as blanks to zero out the spectrophotometer.
Senegalia berlandieri comprises a great number of several alkaloids, the most abundant of those are tyramine, phenethylamine and N-methylphenethylamine. The same type of alkaloids were also reported in Vachellia rigidula. Table 16.2. Total alkaloids found in dried leaves
purpose the propose of this experiment was too see if the chemical reaction of a enzyme can be made faster. Hypothesis I think that a warm environment would be best to make an enzyme’s reaction faster. because a protein can move faster in heat.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
Function of Restriction Enzymes: Restriction endonucleases cleave the phosphodiester bond between an adjacent phosphate and deoxyribose group in the phosphate backbone of the DNA. The active site of the endonuclease perform this cleavage by binding to the side chain of certain amino acids to the phosphate group through a chemical bond. This dissolves the preexisting bond between the deoxyribose sugar and the phosphate resulting in a breakage with in the DNA chain at a specific location. (3, 7) One characteristic feature of restriction endonucleases is that they cut at a very particular site having a specific DNA sequence. This specific sequence that allows the enzyme to attach is known as the recognition site.
Claim This lab was conducted to test the effect of adding hydrochloric acid (HCl) to the reaction between hydrogen peroxide (H2O2) and the enzyme catalase, which is and enzyme found in liver (NOAA Office of Response and Restoration). Our group first hypothesized that if 2 milliliters (mL) of hydrochloric acid was added to a mixture of 2 mL of liver extract and 2 mL of hydrogen peroxide, then the acid would intensify the reaction between the hydrogen peroxide and the catalase and cause increased bubbling. Hydrochloric acid is strong and highly acidic (Merriam-Webster), leading our group to believe that it would create more bubbling. However, since the evidence collected did not support this research, adding hydrochloric acid to the liver extract
Alkaloids, to many, are solely “some set of chemicals.” Prior to reading the section on various alkaloids in Napoleon’s Buttons, I was one of those people who did not necessarily understand what an alkaloid was and why it was significant in my life. By definition, an alkaloid is “any class of nitrogenous organic compounds of plant origin that have pronounced physiological actions on humans.” Alkaloids are all around us, yet most people have no idea what substances are alkaloids and where they come from. Medicines such as morphine, chemicals in recreational drugs such as the nicotine in tobacco, and the caffeine in the tea and coffee we drink all are alkaloids.
Milewski, 1990). Some plants contain secondary plant substances which are by-product chemicals of primary metabolism not directly involved in photosynthesis (Krebs, 2008), Secondary plant substances include a class of organic compounds with a nitrogen base known as alkaloids (Merriam-webster, 2015). Well-known alkaloids are nicotine, tobacco and caffeine which make the plant repugnant (Krebs, 2008) thus lowering the rate of herbivory. Further research has been conducted since the year 2008 which confirms the statements on plants made by Krebs.
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
In addition, he also discovered quinine an alkaloid obtained from the bark of several species of the cinchona