Caffeine Lab Report

I. Introduction

Caffeine as we all know is a kind of stimulant in our central nervous system and classified as methylxanthine. It also stimulates some other parts of our autonomic nervous system. This substance was mainly found in the seeds or leaves of a certain plant. A well known example of this plant is the coffee bean. Thus, to calculate the caffeine content of soft drinks, we may use the process of HPLC. And as we define HPLC (High performance liquid chromatography) and formerly known as high pressure liquid chromatography, is a classical quantitative analysis method by which we use pump in a pressurized liquid to extract the mixture and get the sample of caffeine content of soft drinks. Through this method we will able to analyze the caffeine contain. There are disadvantage and advantage of having caffeine in soft drinks by ingesting it. It brings alertness and awakens our system and repel adenosine

Then in the mobile phase is mostly where the carrier is preventive. And it is liquid because it carries all the dissolve materials we are conducting and separate from each other. Thus in this process we can identify the caffeine content of the soft drinks. Next is the stationary phase that can be either a solid or liquid. An example of solid is silica beads. But here we use a liquid sample. Then the characteristicis classified to polar and nonpolar. We separate these to know the chemical compunds of the our sample soft drinks. For us to determine the caffeine e in soft drinks. Next we will go to the separation mechanism that is shown in. figure 2.1 (The HPLC system). We start in the mobile phase and ends to a detector where in these detectors is connected to a computer. Then this computer it in a graph (see figure 2.2) to show our sample soft drinks caffeine level content which has a chemical name of