Purpose / Objective(s):
1. To measure and determine hand washing efficacy of various regimens against forms of common microscopic organisms.
Hypotheses:
1. The regimen of hand washing, soap scrub with > 10 sec of rigorous hand rubbing followed with paper towel drying is the most efficacious method among the chosen hand washing regimen.
2. The regimen of Purell with active antibacterial ingredient and a course paper towel is also efficacious.
3. The regimen of paper towel soaked with Betadine, scrubbed and followed with an alcohol wash down then air dried is the least efficacious of the three regimens.
4. The lips that came in contact with the left sleeve of a sweat shirt had contact with the lab countertop, and the building’s hand rails will show some growth of
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Determined three sites / conditions for sampling
• Pointer finger / back of an iphone covered
• Middle finger / front pocket of a backpack
• Ring finger / left Crocs sandal
2. Prepared samples
3. Placed samples on the periphery of standard agar culture plates – see Figure # 1
4. Prepared a similar sample from self-kiss
Hand Washing Efficacy
1. Determined/chose/selected three hand washing regimen(s) – see Table # 1
2. Washed each finger with the given regimen
3. Placed the washed samples on the corresponding quadrant of the agar plate near the center
4. Allowed the cultures to incubate at body 37 degrees C temperature for 7 days
5. Observations were made of each culture with the use of a dissection microscope to characterize the microorganism/colonies present in the agar plate.
Kiss
Nothing
Culture of the kiss was allowed the same growth conditions and observation
Instrumentation and materials
1. AO Dissection Microscope
2. Agar culture plate
Experimental Design and Results
See Table 1 and Table 2 and Figure 1 for detailed experimental design and
Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided
In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
If a sponge or an instrument fell on the floor it was washed and squeezed in a basin of tap water and used as if it were clean. Our silk to tie blood vessels was undisinfected.... The silk with which we sewed up all wounds was undisinfected. If there was any difficulty in threading the needle we moistened it with bacteria laden saliva, and rolled it between bacteria-infected fingers. We dressed the wounds with clean but undisinfected sheets, shirts, tablecloths, or other old soft linen rescued from the family ragbag.
This episode was about a man named Joshua who was very successful and pretty much had life figured out. He had a six-figure salary managing 14 different convenience stores. He also had a beautiful home, wife, and kids by the age of 21. But Joshua was overweight and at age 27 he decided to have gastric bypass surgery to help him with his weight loss. After the surgery, he wasn 't losing weight as quick as he wanted and he had back issues which prevented him from exercising.
Tools to be sterilized regularly using barbicide and the use of the UV
The primary prevention is the best way to eliminate the potential for exposure. Since hand washing is the most effective mean of spread of infection, it would be my primary goal to increase the compliance of hand hygiene among healthcare workers, but also an extensive education of patients and family members on hand washing before and after touching the patient as well as afar any contact with any potentially contaminated materials (surface, body fluids or respiratory secretions). Mandatory education of patients, visitors and healthcare workers, across the system as well as cross department compliance practices are single best mean of preventing the spread of infection. For example, every patient and family member can be educated about hand hygiene, use of PPE-personal protective equipment (face mask, gowns and gloves). Although, the practices are already being utilized, I believe the compliance is poorly monitored.
If you don’t wash your hand you don’t know what kind of bacteria got in your hands. You can eventually die if you get a bad bacteria. That was one reason why many colonist
Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
Jaspreet Singh Professor Paratore Biology 1 November 1, 2014 Spectrophotometry Identifying Solutes and Determining Their Concentration Statement of the Exercise or of the Problem The purpose of the lab experiment was to attain the following objectives: • Learning to Operate the Spectrophotometer • Construct absorption spectra for cobalt chloride and chlorophyll. Hypothesis If greater and higher concentrations of cobalt chloride are added to each solution then greater amounts of light would be absorbed by each solution. Thus a liner relationship will result in which the absorbance of a substance would be proportional to its concentration, which will be depicted, in a linear graph.
Presently, health care practices are mainly based on evidence that is created through research. It is also clear that some sources are not as good as others. Using distinct knowledges and research, evidence-based practices are developed. With the PICOT question already identified, this paper will proceed to the research part. Hand hygiene is said to be the most operative answer to avoiding the development hospital-acquired infections (HAIs).
The sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used. The plate was incubate with lid up on the bookshelf at room temperature for 48 hours.
INTRODUCTION: Quick look at your hands do you see them. (attention) Do I see what you might be asking? Well the millions of bacteria that are currently hanging out on your hands.
Larson & Bobo (1992) state that while ethanol and isopropanol may reduce viable bacterial counts on hands, this does not obviate the need for hand washing with water and soap whenever hands are dirty or visibly contaminated with proteinaceous materials, e.g blood (Larson, 1988). Several studies recommend, the use of gloves and routine hand washing with soap and water after care of CDI patients rather than alcohol based hand rub for mechanical removal of spores from hands (Siegel et al, 2007) (Haut, 2008) (Vonberg et al. 2008).