Ionisation Of Amino Acid And Ph Lab Report
Explain the relationship between the ionisation of amino acids and pH |Structural diagram of the neutral structure| |Structural diagram of positively charged structure| |Structural diagram of negatively charged structure| Explain how the form of an amino acid, whether positively charged, negatively charged or neutral, depends of the pH of the solution ? If you increase the pH of a solution of an amino acid by adding hydroxide ions, if this is done then the hydrogen ions will then be removed from the -NH3+group . . To test if it is now a negative ion a process called electrophoresis.although it is colourless its position can be detected using ninhydrin. If the amino acid has dried and then heated gently it would appear as
Enzyme Lab Report
250 Words | 1 PagesThe human body consists of enzymes which allow foods to be broken down and enable organisms to build chemical substances such as other proteins, carbohydrates and fats that are necessary for life. This experiment will be testing the enzymatic reactions with toothpicks. This experiment depicts the process when a substrate binds with an enzyme. However, only a specific substrate can attach to a specific enzyme. This allows the substrate to attach to the enzyme and then disconnect the enzyme overall causing the substrate to break in two.
Example Of Qualitative Research Paper
1591 Words | 7 PagesIf NH4Cl increases the pH of the water, it shows that NH4Cl increases the pH of the blood and if NH4Cl decreases the pH of the water, it shows that NH4Cl decreases the pH of the
Glutamates Lab Report
133 Words | 1 PagesGlutamate is an excitatory nuerotransmitter that binds to receptors and lets sodium flow into the cell. Arrival of sodium ions depolirizes the membrane to threshold potential, and initiates action potential. From the Exam tables we see that humans table show that it took the membrane a longer time to reach the membrane potential compared to the time that it took in jedi's. My hypothesis is that humans glutamate receptors are somehow not folded compeletely, and making the binding site for glutamate not to be so stable. This in turn is shortening the length of time that glutamate is bound to the receptor and a shortens the time that glutamate receptor is open.
Complex Macromolecules: The Five Types Of Proteins
744 Words | 3 PagesR groups determine the chemical properties of the amino acids. The simplest amino acids have hydrocarbons as side chains which are neutral and non-polar or hydrophobic. They are not soluble in water. Amino acids with a polar R group are neutral and polar or hydrophilic. They are more soluble in water.
Alkaline Phosphatase Enzyme Lab Report
2575 Words | 11 PagesIntroduction 1.1 Aim: To determine the kinetic parameters, Vmax and Km, of the alkaline phosphatase enzyme through the determination of the optimum pH and temperature. 1.2 Theory and Principles (General Background): Enzymes are highly specific protein catalysts that are utilised in chemical reactions in biological systems.1 Enzymes, being catalysts, decrease the activation energy required to convert substrates to products. They do this by attaching to the substrate to form an intermediate; the substrate binds to the active site of the enzyme. Then, another or the same enzyme reacts with the intermediate to form the final product.2 The rate of enzyme-catalysed reactions is influenced by different environmental conditions, such as: concentration
Acid Base Balance
821 Words | 4 PagesAcid and base balance of body is normally strongly controlled, keeping the arterial blood pH between 7.38 and 7.42. Acid and base is also important in human homeostasis regarding the appropriate balance between acids and bases; this is also called pH. The body is very sensitive to its pH level, so strong mechanisms exist to maintain it. When pH is imbalanced the protein become denatured and digested, enzymes lose their ability to function, and can cause death. Metabolic Acidosis and Alkalosis: Metabolic Acidosis and Alkalosis are caused by an imbalance of acids or bases and their excretion by the kidneys. Metabolic acidosis produces when the amount of acid in the body is increased through absorption of a substance that can be broken down to an acid.
Sodium Accumulation Lab Report
632 Words | 3 PagesNonosmotic Sodium Accumulation and Salt Sensitive hypertension As we discussed earlier, high sodium diet causes water retention and increases the blood pressure by increasing extracellular fluid volume. In contrast to this traditional view, Heer et al. 2000 performed an experiment in which they showed that consumption of high dietary sodium causes a relative shift of fluid from interstitial compartment to intravascular space. They selected 32 healthy male subjects and provided them with a diet rich in sodium (50meq/day to 550meq/day)for 7 days. They observed that total body water present in the subjects didn’t increase.
Acid Base Neutralization Lab Report
1001 Words | 5 PagesAcids are proton donors in chemical reactions which increase the number of hydrogen ions in a solution while bases are proton acceptors in reactions which reduce the number of hydrogen ions in a solution. Therefore, an acidic solution has more hydrogen ions than a basic solution; and basic solution has more hydroxide ions than an acidic solution. Acid substances taste sour. They have a pH lower than 7 and turns blue litmus paper into red. Meanwhile, bases are slippery and taste bitter.
Lab Report On Acid Base Titration
1346 Words | 6 PagesStrong acids and strong bases are strong electrolytes and are assumed to ionize completely in the presence of water. Weak acids however, only ionize to a limited extend in water. Any weak or strong acids when in contact with any weak or strong alkali will start to undergo neutralization regardless of their volume. When an indicator which is present in the acid-base mixture and have experienced colour change, it indicates that the mixture is in right proportions to neutralize each other and is also known as the equivalence point.
Protein Synthesis Essay
1346 Words | 6 PagesCoomassie G-250 is doubly protonated in acidic conditions and appears red in color; however, when bound to the basic amino acids of the protein, the dye shifts to the anionic blue form. As the protein and dye interact, an electron is donated to the charged groups within the protein so that the protein structure is disrupted and the hydrophobic pockets are exposed. The sulfonic groups of the dye bind to the amines within the exposed hydrophobic pockets to shift the dye to the anionic blue form. This color change is measured spectroscopically and is a direct correlation to the concentration of protein.10 Consequently, the BSA standard is used for comparison, because the basic and aromatic amino acid compositions are similar between the BSA standard and alkaline
Clotting Cascade Case Study
1075 Words | 5 PagesThe amino acid glutamate normally contains a single negatively charged COO-, or carboxylic acid group in its side chain. An additional COO- group allows glutamate to bind positively charged calcium ions much more effectively. Vitamin K is an essential cofactor in the enzymatic reaction producing gamma
Gluconeogenesis
1482 Words | 6 PagesIf the amino acids be existent in the H2A+ generate initially, the titration with a strong acid and a strong base determination lead to the formation of the neutral zwitterion form (HA+/-) previously acquiescent the anionic usage (A-). The titration curve determine illustration two buffer regions and two inflection points specifying the equal points in the titration. The titration curve for a 25.0ml aliquot of the protonated form of 0.100 M glycine (H2A+) with 50.0 mL of 0.100 M HCl solution in note that the change around the another equivalence point is greatly less important than the primary
Hydrochloric Acid And Sodium Thiosulphate Lab Report
1539 Words | 7 PagesIntroduction The goal of the experiment is to examine how the rate of reaction between Hydrochloric acid and Sodium thiosulphate is affected by altering the concentrations. The concentration of Sodium thiosulfate will be altered by adding deionised water and decreasing the amount of Sodium thiosulphate. Once the Sodium thiosulphate has been tested several times. The effect of concentration on the rate of reaction can be examined in this experiment.
Benzoic Acid Lab Report
767 Words | 4 PagesAbstract The unknown concentration of benzoic acid used when titrated with standardized 0.1031M NaOH and the solubility was calculated at two different temperatures (20◦C and 30◦C). With the aid of the Van’t Hoff equation, the enthalpy of solution of benzoic acid at those temperatures was determined as 10.82 KJ. This compares well with the value of 10.27KJ found in the literature.
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