Rp Uppc Case Study
To optimize the RP-UPLC parameters and reach a good resolution and peak Tailing for Lamivudine,Abacavir and Dolutegravir, many chromatographic parameters were tested. Several mobile phases of different ratios were analysed to get that good resolution ,peak shape ,provided sufficient selectivity for the drugs. The phosphate buffer provided a higher sensitivity and selectivity than other buffers did. Using methanol and acetonitrile as organic components shown results in higher sensitivity, but varying the amounts of methanol and acetonitrile in the mobile phase affected the resolution , tailing factor theoretical plates and run time. Varying the pH of the mobile phase resulted in poor peak shapes, not showing good resolution. So we introduced potassium dihydrogen phosphate and di potassium hydrogen phosphate into the mobile phase to …show more content…
The limit of quantification (LOQ) and limit of detection (LOD) were determined based on the slope and the standard deviation of the response using the signal-to-noise ratio (S/N) as per ICH Guidelines Q2(R1) 2005. The LODs for Lamivudine, Abacavir and Dolutegravir were found to be 0.021, 0.330 and 0.038µg/mL respectively, and the LOQs for Lamivudine, Abacavir and Dolutegravir were 0.056, 1.320 and 0.095 µg/mL respectively. (table 6 ) Robustness Robustness of the method was performed by making slight deliberate changes in the analytical methodology like flow rate and solvent ratio. It was observed that this method did not significantly affect in system suitability parameters like USP tailing factor, theoretical plates and resolution, which confirmed that the developed HPLC method is
Eastmamol/540 Week 4
563 Words | 3 PagesThis addition aids in controlling the reproducibility and retention. Separation of the mixture via RP-HPLC can be done using continuous gradient or stepwise to move out the sample components. For every separation, the ideal gradient and volume must be
OWCP Case Study
514 Words | 3 PagesAmend provisions to require OWCP to work with USPS and other agencies to pursue Third Party collection on the behalf of their injured employees. In 2013, OWCP withdrew from the joint agreement for the USPS to pursue Third Party claims. Most interesting is that Third Party collection is an adjunct activity in relation to claim benefits. From the inception of the original joint agreement the between OWCP and USPS in November of 1980, USPS successfully negotiated third party settlements on behalf of USPS employees for 35 years. On March 10, 1985, the parties gave signature to a Memorandum of Agreement expanding the joint third party collect program for an indefinite period.
Dup15q Case Study
160 Words | 1 PagesShort description: Although dup15q syndrome is a disorder that cannot be undone, many treatments are available to help address its symptoms. This page will explain why a multidisciplinary team can help treat dup15q and will also outline potential treatments for dup15q symptoms. Dup15q Treatment Why a multidisciplinary team? Because of the wide range of symptoms associated with dup15q, a multidisciplinary team of doctors can help determine what types of treatments to pursue.
Isp Case Study
484 Words | 2 PagesOn 4/18/20 SC completed a monitoring telephone call with Pa. SC asked Pa how is he doing and he reported that he is doing well. The Pa reported that his knees gave out as he was walking to walk up the steps, but no injuries were reported and the Pa did seek any medical treatment. The SC asked the Pa if anyone was present and if so who, and asked if the person (if any) helped him up. The Pa reported that his girlfriends were present and she helped up.
Bromohexane Lab Report
1111 Words | 5 PagesIt forms a complex with HBr and extracts it from the aqueous phase into the organic phase where the alkene is. This dehydrates the acid, making it more reactive so that the addition reaction is possible. Rapid stirring is required in order to maximize the surface area
Quetiapine Fumarate Case Study
3223 Words | 13 PagesThe mobile phase used was a mixture of ammonium acetate buffer and acetonitrile at a ratio of 400:600. A flow rate of 1 mL/min was maintained, and the detection wavelength was 292 nm (22). The required studies were carried out to estimate the precision and accuracy of the HPLC method and were found to be within limits [percent coefficient of variation was less than 15%]. Sample preparation briefly involved 0.4 μ membrane filter through which the sample was filtered, diluted with mobile phase, and 10 μL was spiked into
Unknown Solid Acid
1800 Words | 8 PagesPurpose: The purpose of this lab is to titrate an unknown solid acid (KH2PO4) with a standardized sodium hydroxide solution. After recording and plotting the data, the acid’s equivalence point will be recorded once the color changes. Using the equivalence point, the halfway point will be calculated, which is used to determine the acid’s equilibrium constant. The acid’s calculated equilibrium constant will be compared with the acid’s established pKa value.
Unit 1 And 4 Reaction Lab Report
720 Words | 3 Pages, and Station 5- 2Na3PO4(aq) + KNO3(aq). The lab demonstrated the Solubility Rules when the equations that included Nitrates and Group 1 salts resulted in soluble compounds, and the ionic compounds that included Carbonates, Phosphates, Hydroxides,
Nucleophilic Substitution Reactions Lab
1039 Words | 5 PagesTLC was used to identify the actual unknown product as well as other products/reactants present in the filtered solution. The procedure was conducted by placing a TLC plate in a developing chamber that is filled with a small amount of solvent. The solvent cannot be too polar because it will cause spotted compounds on the TLC plate to rise up too fast, while a very non-polar solvent will not allow the spots to move. The polarity of the spots also determines how far it moves on the plate; non-polar spots are higher than polar ones. After spots on the TLC form, the Rf values are calculated and used to analyze the similarity of the compounds.
Realnetwork Case
606 Words | 3 PagesRealNetwork creates digital media that people can use on a daily basis. RealNetwork products and services consist of RealMedia HD, which people use to stream movies and videos on their mobile devices of choice over the Internet; RealPlayer, which people use to watch videos, photos, and music on their PC; RealTimes, which organizes your photos to create a video story that is set to music and arranges them with different transitions; and GameHouse, which offers a host of games that can connect players on any devices they choose to use. RealNetwork has faced many challenges as they try to expand its brand. One of the challenges that RealNetwork faced is the elimination of their product through the Microsoft monopolization of the digital media market.
Rj45 Unit 5 Case Study
326 Words | 2 PagesTD is responsible for providing at least one suitably sized network circuit to both data centers and terminating at a TDCT controlled firewall or router. This device will provide an Ethernet RJ45 handoff to RPM equipment. RPM will consider the RJ45 as the demarcation point. RPM suggests that this link be at least 100 mb’s and by year 4 it should be at an estimated 1 Gb/s or larger.
Organ Bath Experiment
1445 Words | 6 PagesNext, a basic stock solution was used to prepare various concentrations ranging from 1.0 x 10-8M to 1.0 x 10-1M by serial dilution. The tissue was washed by overflow with reservoir’s solution for 5 seconds to obtain baseline before adding 0.1ml, 0.3ml and 0.5ml for each concentration respectively into the tissue bath. The tissue’s peak response for each final bath concentration(FBC) was measured and recorded. Rmax and EC50 of histamine were recorded.
Batch Precision And Aclofen Case Analysis
2069 Words | 9 PagesWithin Batch Precision and Accuracy for Baclofen Nominal Concentration (ng/mL) LLOQ QC LQC MQC1 MQC2 HQC QC# 20.759 % Accuracy 60.171 % Accuracy 110.406 % Accuracy 506.451 % Accuracy 750.297 % Accuracy 19 19.572 94.28 59.161 98.32 106.354 96.33 485.214 95.81 732.116 97.58 20 20.470 98.61 60.615 100.74 106.350 96.33 478.472 94.48 739.915 98.62 21 19.717 94.98 58.658 97.49 105.322 95.40 482.899 95.35 734.087 97.84 22 19.788 95.32 59.053 98.14 107.084 96.99 481.018 94.98 736.005 98.10 23 19.818 95.47 59.280 98.52 106.976 96.89 478.309 94.44 734.365 97.88 24 19.826 95.50 59.813 99.40 107.306 97.19 488.846 96.52 739.547 98.57 Mean 19.8652 59.4300 106.5653 482.460 736.0058 S.D. 0.31095 0.69049 0.72431 4.09323 3.14091 C.V.% 1.57 1.16 0.68 0.85 0.43
Buffer Lab Report
1344 Words | 6 PagesThe NaH2PO4 and Na2HPO4 powdered were weighted by using weighing machine, followed the mass that has been calculated in step (3). The NaH2PO4 and Na2HPO4 powdered were mix in a 500 mL beaker. 500 mL of distilled water were measured by using a 500 mL measuring cylinder, then is poured inside the 500 mL beaker containing both the powdered.
Hammett Constant And The Hydrophobic (Hansch) Substituent Constant
1027 Words | 5 PagesThe substituents should therefore be hydrophobic and electron donating for maximum activity. • log 1/C is the concentration of drug that has a defined biological activity. The bigger the value of log 1/C , the smaller the value of c, the better the drug. • P is a measure of overall hydrophobicity. • π is the substituent hydrophobicity constant and measures the hydrophobicity of a specific region on the drug’s skeleton.
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