An Investigation of photosynthesis for silver beet. The aim of this investigation is to find the average rate of photosynthesis for silver beet compared to gold beet. Methods DPIP is a measure to help find the rate of photosynthesis. The rate was calculated using the slope of the absorbance/time graphs Appendix a. The rate was then calculated by dividing the change in absorbance over the change in time, an example of this can be found in appendix B. 3 Trials conducted to find out the rate of photosynthesis for silver beet. These trials where trailed against gold beet using a two sample T test to see if the data was of normal distribution (Mosse, 2015). Results To find the normal rate of photosynthesis for silver beet, the calculated average …show more content…
dpip turns colourless when electrons are transported which gives us an estimation on the rate of photosynthesis (Mosse, 2015) Temperature can have a great effect on photosynthesis, as generally the higher temperature the higher rate of photosynthesis. Photosynthesis is a chemical reaction, and as temperature rises so does the reaction, however temperatures above 40 degrees slow the reaction down, this is because the enzymes involved in photosynthesis are temperature sensitive and can be destroyed if the temperature is to great. (Nature, 2012) The aims of this investigation are to test the effects temperature has on the rate of photosynthesis of silver beet. silver beet will be tested against four different temperatures to see how the rate differs. …show more content…
each condition reacted differently to one another based on their temperatures and altered the rate of photosynthesis. Room Temperature did not alter the rate of photosynthesis, this is because it was a normal climate and did not increase or slow down the enzyme reaction within the chloroplast. When the chloroplast was subjected to 80 Degrees, we can assume the protein was broken down, as the active site of the enzyme would become distorted, making the substrate no longer fit, denaturing the enzyme, thus no reaction occurring (Nature, 2012). For this reason we can see why the 37 degrees reaction was slowed. For 0 Degrees the enzyme was slowed but as the initial temperature began to wear off we can see that a reaction began to occur, as the reaction needed heat to occur the light from the lamp provided it with this, so the reaction could
Each were labeled and paired up with one containing the other set of ingredients. To determine if temperature affected the performance of peroxidase each set was isolated in a specific temperature. Two of the test tubes were set in a refrigerator of 4 degrees Celsius, another pair was left to sit in room temperature of 22 degrees Celsius, the third pair was left in an incubator of 32 degrees Celsius, and the last pair was left in a water bath of 60 degrees Celsius. All four sets were left in their designated temperature for 15 minutes. Before the 15 minutes were up the spectrophotometers were set at 470 nm and zeroed out using the blank.
Dependent Variable Rate of Respiration (Rate of which CO2 is produced in ppm/s) The rate of respiration of the germinating seeds can be represented by the rate of which CO2 is produced. The rate of respiration is expected to be affected by the change in temperature of the environment. As mentioned in the hypothesis, the change in the respiration rate is most likely to be due to the involvement of enzymes in the process.
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.
Quinn Nguyen Floating Leaf Disk Photosynthesis Lab Conclusion: Graph your results for all 3 trials on one graph. Label the graph, both axes, and provide a legend to distinguish each trial. What was the rate of photosynthesis for each variable? How many leaves floated per minute? Rate of photosynthesis (leaves/min) Spinach: (2-0)/25 = 0.08
Because carbon dioxide is absorbed by the plant during photosynthesis less carbon dioxide present in the chamber is a sign that photosynthesis is working. The four lights used for this experiment range across the light spectrum on both sides in order to test a wider variety of wavelengths. All lights will be placed directly on the spinach leaf at the same distance so as not to give any spinach leaf a different light intensity, which could affect the data. This experiment will be able to show which light, ranging across the light spectrum, will allow the Spinach to perform photosynthesis more efficiently.
Chemical stress affected the cell membrane of a beet cell, because of the higher amount of ethanol added to the beet. For example, we added 1% ethanol, 25% ethanol, and 50% ethanol to 3 test tubes with 15 mm of beets inside. We left it with no air inside for 30 minutes then tested the absorbance of the ethanol without the beet. The class got roughly 0.273 for the 1% ethanol, 1.205 for the 25% ethanol, and 1.882 for the 50% ethanol concentration. In each solution, the ethanol was a bit redder than the last.
For this lab I will be using water and sucrose to demonstrate the rate of osmosis. In this lab I will be exploring how temperature impacts the rate of osmosis by placing pieces of potato of equal size in solutions of different temperatures and observing the change in mass of potato after a given period of time. The change in mass will indicate the rate of osmosis.
A. Were the seeds in the “N” tube undergoing both photosynthesis and respiration, only photosynthesis, or only respiration? Use your results in Data Table 2 to support your answer. The seeds in test tube "N" underwent respiration. Respiration is always happening.
These findings show that the null hypothesis is rejected, and that there is a major difference between the observed and expected values. It is also observed that in the data in Figure 1.1, as the concentration of the NaHCO3 increases, the greater the production of oxygen. The highest photosynthetic rate is in at a 1.0% concentration of NaHCO3and the lowest in at a 0.1% concentration of NaHCO3. It is also important to note that the 0.4% concentration of NaHCO3 had a greater oxygen production than the 0.6% concentration of NaHCO3.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
Methanol, has a molecule containing CH3OH it being the smallest, ethanol having more carbons and hydrogens than Methanol comes second, CH3CH2OH, and propanol, CH3CH2CH2OH, is the largest of the three molecules. Aim: The aim of this experiment is to see the effects of different alcohols and concentrations on the biological membrane of the beetroot. Research Question: How do different alcohols and concentrations affect the biological membrane of a beetroot? Hypothesis: As the alcohols increase in toxicity and concentration, the damaging and breaking down of the membrane will increase leading to more red pigment being released into the solution causing the absorption of the solute to increase.
Dependent The time taken for the bluish -black color to fade away (color of Iodine solution mix with starch solution ). The rate of enzyme reaction Minutes (min) Table 1.1 – Table shows the controlled variables in the experiment variables Units Measures of controlled variables.
The 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.
Background Information: In this experiment I will be investigating the impact of light intensity on the rate of water uptake, due to transpiration, by attaching a shoot from a leafy plant in the capillary tube of a potometer, and then measuring how long it takes for a bubble to move a set distance. The faster the bubble moves, the greater the rate of transpiration. I will be placing one plant in an environment where it is exposed to high-light intensities, and another plant in an environment where it is exposed to low-light intensities. Transpiration is the process of the transport of water and nutrients up the the plant from the roots to the leaves.
RESEARCH QUESTION Which one has a higher rate or respiration between dicotyledonous (peas) and monocotyledonous (maize) seeds and what is the effect of temperatures (room temperature, 40, 60) on the rate of respiration as determined by oxygen usage estimated with a respirometer? AIM The aim of this experiment is to investigate which seed has a higher rate of respiration and how different temperatures (room temperature, 400C, 600C) affects the rate of respiration of dried, fresh and germinating monocotyledonous (peas) and dicotyledonous (maize) seeds.