Shake c) Drain the lower aqueous layer through the stopcock into the same 250 ml beaker in which the solution had been prepared in steps above. d) Pour the upper solvent layer through the neck of the funnel into a clean 125 ml Erlenmeyer flask. Return the aqueous solution from the 250 ml beaker to the separatory funnel. Add another fresh 20 ml of solvent to the funnel and again extract the aqueous solution as you did in b)
The comb was then placed into the solution to create the wells. Once the gel had solidified (4-5 minutes) the comb was carefully removed. The solidified gel was then placed into the buffer chamber ensuring that the wells were located closest to the negative terminal. This ensures that the DNA will travel through the gel in the direction of the positive terminal. If the wells are placed closest to the positive terminal it will result in the DNA running off the gel and a failed procedure.
We added sodium carbonate until the pH of the mixture was 8. After neutralize, we collected benzocaine by vacuum filtration. We used a Buchner funnel to collect benzocaine. We used three 10 ml of water to wash the product. After the product was dry, we weighed, calculate the percent yield and determined the melting point of the product.
The saturator is the place where dilution process occurs by adding water to it after that flocculating process happens or settle process . the settle process is all about adding a polymer to join with small solid particles then it settle down and in the top almost pure water is existed. The pure water is dosed into the line with out powder to avoid the deposition in the line. There is an extraction pump used to take the settled powder for recycling by adding water or to the sludge
Then it was left to boil under for 1hour. After which round bottom flask was removed from the reflux setup and held first under running room temperature water and then an ice bath until it cooled down enough to comfortably handle it. Next the cooled solution is poured into a 100ml volumetric flask and topped it off to the mark with denoised water. Subsequently, 20ml of this solution was pipetted into a conical flask. To this, 80ml of cold water and 15ml of 2M HCl was added to the conical flask.
Distilled water was pure into the rubber-stopper to make sure the buret was completely filled and to make sure there wouldn't be any bubble. Then the buret was quickly inverted and pressed into a beaker with full of water. Finally after the water cooled down, the water level of the buret was measured inside beaker. This process was repeated for two more
ZPFe (3 mol%) was added to a mixture of a benzoyl chloride (10 mmoL) and an aromatic compound (10 mmoL). The reaction mixture was stirred for the appropriate reaction times at 80 °C (Table 2). After completion of the reaction (monitored by thin-layer chromatography, TLC), the mixture was diluted with Et2O and filtered. The organic layer was washed with 10% NaHCO3 solution and then dried over anhydrous Na2SO4. The solvent was evaporated under reduced pressure and the product purified by column chromatography on silica gel to give the corresponding pure aryl
Ensure that solid is completely dissolved using a stirring rod. Next, a 10 mL beaker is filled with 3 mL of HCl and measure 10 mL of ionized water into a 140 mL beaker. Carefully turn on laboratory burner and start cleaning the Nichrome wire by dipping it into concentrated HCl acid. Hold the Nichrome wire on top of the flame and repeat the step until the wire doesn 't show any color. When the wire is clean, dip the wire again with some of the acid and dip it into the solution with the unknown compound in it.