For this experiment, the proportion to get a CO2 is 2HCl+Na2CO3 = CO2.., which is 2+ Na2CO3 : 1. So as the mole of hydrochloric acid is bigger,
To 0.05 ml of carbonate buffer (0.05 M, pH 10.2) and 0.5 ml of EDTA (0.49 M) was added. The reaction was initiated by the addition of 0.4ml of epinephrine and the change in optical density/min was measured at 480nm. SOD activity was expressed as units/mg protein change in optical density/min. 50% inhibition of epinephrine to adrenochrome transition by enzyme is taken the enzyme unit. Calibration curve was prepared by using 10 -125 units of
This solution was diluted with diluents to gae a concentration of 0.1 mg/ml solution each of Amoxicillin trihydrate. The HPLC method was applied to the solutions and the results obtained were shown in table 4.6.11. System suitability solution: 25.0 µg/mL each of of USP Amoxicillin RS in Diluent. Precision
In Section C, a pipette was calibrated by measuring the water temperature and the density was determined. The average mass of water was calculated and the reading was 20.68g. The value for the relative average deviation was 5.33%. In Section D, the crucible, crucible lid and anhydrous magnesium sulphate were weighed and the % water in magnesium sulphate hydrate was recorded calculated which is 41.56%. In
Another 5-mL test tube, labelled as B, was filled with 1 mL of distilled water. A drop of methyl red was added. Also, a 0.01M hydrochloric acid (HCl) was added in a dropwise manner from a syringe until the color of the solution matches that of the first test tube setup. The volume of the HCl used was recorded for the determination of the ionization constant of
Next, a basic stock solution was used to prepare various concentrations ranging from 1.0 x 10-8M to 1.0 x 10-1M by serial dilution. The tissue was washed by overflow with reservoir’s solution for 5 seconds to obtain baseline before adding 0.1ml, 0.3ml and 0.5ml for each concentration respectively into the tissue bath.The tissue’s peak response for each final bath concentration(FBC) was measured and recorded. Rmax and EC50 of histamine were recorded. Later, 5ml of 1 x 10-6 M of mepyramine was added into the reservoir containing 1000ml of Krebs-Henssleit solution to produce a FBC of 5.0 x 10-9M. It was equilibrated with tissue for 10 minutes by flushing into the organ bath.
The solution were tested by using calibrated pH meter to get the pH value of the solution. Results and Discussions pH ratio between acid and base: 7.3 = 6.82 + x x = 0.48 0.48 = log ([base])/([acid]) 100.48 =base/acid salt/acid = 3.02 There, 1 acid : 3 base calculate number of mole of acid and base to find the mass : molar = mol/L 50 mM = (mol )/(0.5 L) mol = 25 mol number of mole of NaH2PO4 25/4 = acid = 6.25 mol number of mole of Na2HPO4 25/4 × 3 = salt = 18.75 mol to calculate the mass of the acid and base : Mass of NaH2PO4 (6.25 mol)/(119.98 g/mol)=0.052g Mass of Na2HPO4 (18.75 mol)/(141.96
The theoretical yield for Zinc Sulfide is 0.49 grams but the actual yield is 0.38 grams. So if 0.38 is divided by 0.49 and multiplied by 100 then the percent yield for Zinc Sulfide would be 77.6%. When it comes to Sodium Chloride, the theoretical yield is 0.58 grams and the actual yield is 0.45 grams. So when 0.45 grams is divided by 0.58 grams and multiplied by 100, the percent yield would be 77.5% of Sodium chloride. The actual yield is directly taken from the mass of the products in the experiment while the theoretical yield is determined by using stoichiometric calculations.
Decomposition of Aspirin Studied with UV/Visible Absorption Spectroscopy Aims: To determine the concentration of salicylic acid, formed from the hydrolysis of Aspirin, at regular intervals using the UV/Visible Absorption Spectroscopy From the concentration of salicylic acid, concentration of Aspirin to be determined using an equation Calculate the rate constant of this reaction and its order from a plot of graph of ln(aspirin) vs time Discuss the overall flaws and improvements to the experiment Results: As per schedule1, 0.212g of aspirin was added to 50 ml boiling water to form salicylic acid in a 100 ml flask, of which 1 ml was then pipetted to a 50 ml volumetric flask at the 5th min. Following an ice bath, the solution was mixed
The absorbance of the mixture was measured at 30s (A1), 5min and 6min intervals. The value A2 was obtained by averaging the values of the 5th and 6th minute. b. Urea 750 ml of urea reagent 1 and 450ml of urea reagent 2 was pipetted into the same 1ml cuvette. Following that, 12ml of patient's sample was transferred into the cuvette. The absorbance of the mixture was measured at 30s (A1), 5min and 6min intervals.