Finally the hexane layer was washed with 100 ml water and then evaporated to dryness with a vacuum rotary evaporator. The concentrated extract was then passed through a chromatographic column (30 cm x 10 mm i.d) containing 2 g florisil (lower) and 1 g sodium sulphate (upper) which is pre wetted with hexane: acetone (1:1). OCPs were eluted with 25 ml hexane: acetone (1:1).The solvent was evaporated using rotary evaporator and final volume was adjusted to 5 ml, which is used for GC analysis. All the sediments were analyzed for HCH and
Water was removed from the dish, and the Cu was then washed thrice with 5 mL deionized water, and decanted between washings. It was then similarly washed thrice with 5 mL of ethanol. The evaporating dish was then placed on the steam bath to dry until the Cu had a sand-like texture. The bottom of the dish was dried, it was cooled to room temperature, and then its mass was measured and recorded. All the solutions were disposed of in the liquid waste container, the Cu and Al were disposed of in the solid waste container, and the apparatus was cleaned and put
The solution was discarded into the waste bin, and the materials were washed. The second reaction in Part B, sodium hydroxide and ammonium chloride, began by saving the data from the first reaction and setting up the LabQuest to new data collection under the same conditions as the first reaction. The cups were restacked and placed in the beaker. Using a graduated cylinder, 50mL 2M NaOH was added to the cup. The cup was then covered and the temperature probe inserted.
Place melted agar solution and pre-warmed 2x culture medium in an ice bucket filled with hot tap water (42 °C). Also place a 50 ml conical tube in a tube holder in the ice bucket with hot water. Transfer bucket to cell culture hood for subsequent steps. 3. For the bottom layer of agar, you will need 1.5 ml of a mix of agar and medium per well of a 6-well
Reflux condensation was performed with use of a heating mantle and retort stand. A total of 20 minutes was allocated for reflux, starting when the mixture first began signs of boiling(T0), to when the allocation of time was depleted(T20). The mixture was filtered into a 50mL conical flask, and 10mL of 100°C water. The extract was subsequently allowed to cool to room temperature, and decanted into a separating funnel. Liquid-Liquid (Polar-Nonpolar) extraction of DCM In a fume hood, 8.0mL of DCM was added to the separating funnel, capped and gently mixed.
The drying agent was discarded when the mixture was filtered. The dichloromethane in the mixture was evaporate when the mixture was heated on a hotplate of 120 degrees C. It was heated until it stopped bubbling and a small oil layer remained. Petroleum ether (15 mL) was added, mixed, and cool in an ice bath. The product was vacuum filtrated, keeping the precipitate. The precipitate was further dried by utilizing a drying
Then it was left to boil under for 1hour. After which round bottom flask was removed from the reflux setup and held first under running room temperature water and then an ice bath until it cooled down enough to comfortably handle it. Next the cooled solution is poured into a 100ml volumetric flask and topped it off to the mark with denoised water. Subsequently, 20ml of this solution was pipetted into a conical flask. To this, 80ml of cold water and 15ml of 2M HCl was added to the conical flask.
Once the cola starts to boil, continue to boil it for another 10 minutes so that the carbon dioxide is removed. When the cola has finished boiling, cool it in an ice bath and pour the cola back in the volumetric flask and use distilled water to fill the flask to compensate for the evaporated water. Using a volumetric pipette, transfer 60ml of the cola to a beaker and put the magnetic stirrer in the beaker. Submerge the conductivity probe in the cola. Fill up the burette with NaOH
From the Unknown tube professor Cooper gave me, I scratched a little on the slant surface with the sterilized inoculating loop. Then I place it on a clean prepared slide which already had a slight drop of water. The two substances are mixed together in the middle of the slide and let dry completely. One extra step of “heat fix” is necessary to adhere everything to the surface of the slide. To start gram staining, I slightly pour crystal-violet all over the slide and let it sit for 30 seconds before wash it off with water.