Alicyclobacillus Research Paper

Seven strains of Alicyclobacillus were cultured independently with Alicyclobacillus acidocaldarius medium (AAM) broth in conical flasks with 100 mL headspace and caped with hydrophobic fluoropore (PTEF) membrane incubated for 24¬–48 h on shaker (Fine mixer SH2000 orbital shaker, Korea) at 120 rpm with their specific growing conditions and enriched media (Table 1). Then, 5mL of each bacterial strain culture from first round shaking transferred into a new liquid medium for a second shaking culture. One hundred fifty milliliters (150 mL) of each bacterial cultivation liquid was harvested at their stationary phase with centrifugation at 3600 g for 20 min at 4°C. The supernatants were discarded; the leftover bacterial cells (106cells/mL) were washed

After incubation sporulation was confirmed by microscopy following staining with 5% malachite green and 0.5% saffron malachite green. After reaching more than 80% of sporulation, spores were harvested by a mild agitation of Petri plates using a glass spreader after adding 5 mL of distilled water. (Torlak, 2014; Evelyn & Silva, 2016). The spore suspensions were centrifuged (3600 g, 10 min) and washed three times with sterile distilled water. The concentration of spores in the final suspension, determined by plating 100 μL of appropriate dilutions onto AAM agar, was adjusted to 106 spores/mL with sterile distilled water and stored at 4°C until used. To obtain HI cells and spores, each pellet (cells and spores pastes) of all Alicyclobacillus strains were separately, autoclaved at 121°C for 20 min and freeze-dried (vacuum degree, 5 mTorr [79.3 Pa]) by a vacuum freeze-dryer (MCFD5505, SIM International Group Co. Ltd., Beijing, China) at -54°C for 24 h to constant

Adsorption isotherms study Many equations have been published to describe the equilibrium adsorption isotherms relationship between adsorbed and unadsorbed toxin amounts (Sharma et al., 2017). For equilibrium adsorption isotherms study, the data was carried out by placing 150 mg of HI cells-A2 and HI spores-A2 into 10 ml PAT solutions at 50–250 μg/L at different temperatures (20, 30, and 40℃). In this study, Adsorption isotherms were fit to Langmuir and Freundlich models to provide the best description of PAT adsorption (Freundlich, 1906) and the R2 value was chosen as the best-fitting model.

The Langmuir isotherm model was based on monolayer adsorption is given as by follows ing equation (Langmuir, 1918):

C_e/q_(e ) = 1/(q_m K_(L ) ) + C_e/q_m