After the 30 minutes is done, the tests tubes are then immersed in a 100 degrees Celsius methanol water bath for 15 minutes. Once the samples become frozen, put in a lyophilizer at a temperature of -109 degrees Celsius. The samples are allowed to completely dry. After 6 hours remove from the lyophilizer. Acetonitrile at a PH of 7 (neutral) is added to each of the test tube samples.
The hydroquinone metabolite purified from B. methylotrophicus MHC10 was evaluated for its antibacterial activity against a panel of several Gram-positive and Gram-negative bacterial pathogens. The zone of inhibition was used to evaluate the antagonistic activity of the metabolite. The standard antibiotics Ampicillin and Gentamycin were used as the positive control. Both antibiotics showed high antagonistic activity against all test pathogens. But in the case of P. aeruginosa, the hydroquinone treatment showed little high zone of inhibition than ampicillin Lee et al.,  studied the antimicrobial activities of the purified prodigiosin and cycloprodigiosin against B. subtilis KCTC 1914, E. coli KCTC 1924, Salmonella typhimurium KCTC 1926, S.
DMF was used as a solvent and AIBN (0.5% w/w of total monomer) as free radical initiator .The reaction was carried out at 70±2° C for 6 hour with constant stirring. After completion of the process it was cooled to room temperature and resultant polymer solution was poured in the large amount of methanol with stirring when polymer precipitated out. It was filtered and washed with methanol. The polymer was purified by repeated precipitation using methanol from solution in DMF and then it dried. 2.3 Preparation of PS
Plant material and extraction 300gm dried powder of seed was weighed & taken in a aspirator (2.5L). Before placing powders into the aspirator, the jar was washed properly with acetone and then dried. 800ml of solvent i.e. methanol was added gradually. The container with its content was sealed & kept for 20 days with occasional shaking & stirring.
The other additives that showed great decrease in aging are Solprene 1205 and Calprene 6120. Solprene 1205 seemed to be working well with the 64-22 binder and Calprene 6120 showed high reduction in aging with 67-22 binder. Frequency sweep tests were performed on the binders modified with antioxidant additives to learn about the impact of these additives on linear viscoelastic properties of the binders. The testing was done on both unaged and PAV aged samples. The frequency sweep test was done at 7 temperatures: 10,20,30, 40, 50, 60 and 70ºC and 10 frequencies at each temperature.
According to Dr. Thomas Lee of Harvard Medical School (1), the ingredients are chiefly an amalgamation of stanols and sterols, and include campesterol, beta-sitosterol and stigmasterol. Studies have shown that a combination of plant based stanols and stenols helps lower bad cholesterol (LDL) in the body by preventing the absorption of LDL in food. The active ingredients work by drawing LDL from the bloodstream, then converts the cholesterol into hormones and bile acid. Plant stenols and stanols look similar to Cholesterol and are very effective in blocking the uptake of cholesterol into the body. Research has shown that taking 2g of stanols or sterols from plant sources can reduce the LDL by as much as
Extraction of β-caryophyllene Ground cloves will be used to isolate β-caryophyllene via steam distillation. 5g of ground cloves will be taken in a 500mL RBF(Round bottom flask) with boiling stones, 40mL of dH2O, and 3 to 4 drops of an antifoaming agent (to prevent violent boiling). Then, the contents of the flask will be heated on a heating mantle for an hour and followed by condensation of the distillate through a water jacket. Then allow it to collect in a graduated cylinder. Steam distillation will allow the clove oil to co-distill with the water, which take place at a least temperature than the boiling temperature of the individual solutions.
The purpose of the catalase test was used to identify the catalase positive bacteria (staphylococci) and catalase negative bacteria(streptococci). The results were as follows: Staphylococcus aureus (control)- catalase positive Enterococcus faecalis(control)- catalase negative Nostril Microflora on NA- catalase positive Nostril Microflora on PYCa- catalase negative Nostril Microflora on MSA- catalase positive Figure 1. Staphylococcus aureus gram stain control Figure 2. Enterococcus faecalis gram stain control Staphylococcus aureus and Enterococcus faecalis were used as control in both catalase test and gram staining Figure 3. Nostril Microflora on NA plate-gram stain Figure 4.
In the round-bottom flask (100 mL), we placed p-aminobenzoic acid (1.2 g) and ethanol (12 mL). We swirled the mixture until the solid dissolved completely. We used Pasteur pipet to add concentrated sulfuric acid (1.0 mL) to the flask. We added boiling stone and assembled the reflux. Then, we did reflux for 75 minutes.
While, the anti-inflammatory activity was assessed by two different models; the carrageenan-induced paw edema and the carrageenan-induced peritonitis in which the levels of total leukocyte count (TLC), neutrophil count, prostaglandin E2 (PGE2) and interferon gamma (INF-γ) were measured in the peritoneal exudates. The results revealed significant protection in all the treated groups; however, the combination of EVOO with IBU at its therapeutic dose showed superiority over the two compounds when used separately. Conclusion: Using extra virgin olive oil with the therapeutic dose of ibuprofen showed synergistic effect in controlling the cardinal signs of acute inflammation rather than using the NSAIDs
The dried roots of Inula racemosa were pulverized and sieved with 100 ~ 200 mesh. The herb powder was placed into a glass bottle. Ultrasound-assisted extraction was carried out in an ultrasonic cleaner RK102H (Bandelin sonorex, Germany). The powder of Inula racemosa was extracted three times under the following conditions: the ratio of material to solvent was 10:1, undergoing ultrasonic treatment 30 minutes at 25 °C, 100 kHz /450 W.31 Before large extraction, a small-scale extraction experiments were carried out: 95% ethanol and ethyl acetate as the extractive solutions was investigated, respectively. The extraction efficiency was evaluated according to the percent content of AL and IS contained in the dried roots of Inula racemosa and calculated