Propionibacterium Acnes Strain Extract Report
(Voucher specimen No. 1246). Propionibacterium acnes Strain (MTCC 1951) was procured from (MTCC) IMTECH, Chandigarh. Preparation of extracts and fractions The plant materials were dried and ground to a coarse powder. The ground plant powder (300 gm) was extracted with (80%) methanol by soxhelation for 48 hours. The extract was filtered through Whatman filter paper and concentrated under reduce pressure and transferred to pre weighed China dish and stored in vacuum desiccators until constant weight was obtained. The antimicrobial screening of extracts was laid down for further purification of potent extract. The potent extract was successively macerated with petroleum ether, ethyl acetate, chloroform, n-butane and water (according to solvent …show more content…
The extract of the plant Rubia cordifolia was found to have maximum antibacterial activity in comparison to other three plants i.e Tephrosia purpurea, Viola tricolor and Serenoa repens. The results obtained in the evaluation of the antibacterial activity of the different extracts against P. acnes are listed in Figure 1 and Table 1. The extracts had shown zone of inhibition against bacteria P. acnes but methanolic extract of Rubia cordifolia had shown maximum zone of inhibition against bacteria i.e. 14.0 mm and 24.0 mm in concentrations 1 mg/ml and 10 mg/ml respectively. Pet. Ether fraction showed the most potent antimicrobial activity in different dilutions with time interval of 4 hrs is listed in Figure 2 and 3. The sub fraction D had shown highest anti P. acnes activity with zone of inhibition 26.0 and 32.0 mm at concentrations 1 mg/ml and10 mg/ml respectively are listed in Table 2. The fraction D was further fractionated with the help of sub-column. Six different fractions were collected out and evaluated for antibacterial activity. The results thus obtained were similar to that of fraction D is listed in Table 3. The results for the phytochemical screening and TLC fingerprinting profile are listed in table 4 and 5. The minimum inhibitory concentration of sub
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