The tube was placed back in incubation for 96 more hours to observe any more positives. 2.10 Catalase Test A trypticase soy agar plate was used and after incubation, four drops of 3% Hydrogen Peroxide was added to the plate to flow over the bacterial growth. A presence of bubbling was observed. 2.11 Starch Hydrolysis A starch agar plate was inoculated with a streak of the unknown bacteria and then incubated. On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids.
We then slowly added 25ml of chilled deionised water to the filtrate to initiate crystallization by using a measuring cylinder and a dropping pipette, once we had done this we left it for about 10 minutes to allow crystallization at room temperature. We then weighed a filter paper which we will use later in the experiment. We then collected the crystallized acetylsalicylic acid by vacuum filtration in a Buchner funnel and washed the product with a little ice-cold water. We then pre-weighed a clean, empty watch glass and labelled it with our initials and the date, we did this do we could easily identify that it was ours when we go to weigh it with the crystals on. We
Then an estimated (by trial and error) 1-3 grams of hydrated copper sulfate was added to a crucible with the lid on top. The total mass of the hydrated copper sulfate was recorded by subtracting the total mass of the crucible, lid, and sample from the mass of the crucible and lid (described in table 1.3). By attaching the wire triangle to the ring, the crucible was able to sit securely while having the bunsen burner underneath. Lighting the burner once again, each substance was heated for several minutes until estimated that the compound had changed color. Once a prevalent color change had been observed at approximately 4 minutes (blue green color) the crucible was set on the counter using the tongs to cool for approximately 5 minutes.
The solution of liquid and sold is decanted through the filter paper in the funnel. The solid is trapped by the filter and the fluid is drawn through the Buchner funnel into the glass flask by the vacuum. While starting your scientific experiment always remember a vacuum trap and keep it clean so it does not get contaminated. The technique is faster than gravity filtration. Vacuum filtration generally involves the use of vacuum filter flask, water pump, filter trap and sintered glass crucible.
This study was conducted with a partner, since some parts of the experiment were able to be done simultaneously. One partner prepared a TLC developing jar by pouring a small layer of 4:1:1 propanol/acetic acid/water into a developing jar. A solvent wick was made by wetting a piece of filter with the solvent, and it was placed in the jar. A silica coated TLC plate was obtained, and a spotting line was carefully drawn approximately 1.5 cm from the bottom of the plate using a pencil. Extra care was taken to not touch the plate with bare skin.
The first of the four test was a Gram-stain. Once the experiment was completed the slide was then placed under a microscope where it was then categorized as Gram-negative bacilli. Its pink color when viewed through the microscope indicated that the test was Gram-negative and its rod shape indicated the morphology was bacilli. With these results the next test to be conducted was the citrate utilization test. A citrate tube was inoculated with P. aeruginosa and incubated at 37 degrees Celsius at 24 hours.
CO2 levels were measured using a CO2 sensor. The sensor was given time to warm up then placed in a glass chamber with a sample from one of the treatment groups or a sample of control. CO2 levels in ppm were collected every four seconds for ten minutes by the sensor. The data was divided by the weight of the sample used to generate it, to give the respiration rate per gram of sample. The data was then graphed and the slope of the line of best fit for the data points was found.
Lastly, it told us to repeat the same steps until we had three calcium chloride scoops in the beaker and repeat for two more trials for accurate results. To sum up the experiment, it said to record the average change in temperatures to the class averages to graph a bar graph comparing both of the averages. That’s the procedure on how to conduct the experiment correctly. The averages that my group received for zero scoops were 0.5 degrees Celsius, one scoop was 6.5 degrees
1. What type of macroscopic evidence for chemical change did you observe during this experiment? Give at least three different examples. (15 points) Example 1: The baking soda (NaHCO3) mixed with vinegar (CH3COOH) created foam as well as the washing soda (Na2CO3) when mixed with the vinegar. Example 2: The color change when the starch and iodine (I2) mixed.
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour. Then, tests are performed to determine if the products of aerobic and anaerobic respiration are present in the flasks.The citric acid cycle consists of a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and chemical energy in the form of ATP (Biology).
Then carefully measure 25ml of methanol and 25ml of ethyl acetate using a measuring beaker 12. Pour each solvent into its respective labelled mortar 13. Stir each sample for 10 minutes using a stirring rod 14. Leave solutions in the sun for 12 hours, this is to allow for the active ingredients bond with the solvent and form a solution and to allow some of the Methanol and Ethyl acetate to evaporate. 15.
Cellular Respiration Lab Introduction In this lab, the primary investigation was to discover which factors affect cellular respiration. In this particular inquiry, the factor tested was the amount of time the lentil seeds were germinated. This study was performed in order to understand the process of cellular respiration as well as be able to measure and observe gas concentration as a result of impacting factors. Cellular respiration is necessary for life-processes, converting glucose and oxygen into ATP, carbon dioxide, and water, in a series of metabolic reactions. This process can be measured in numerous ways, such as observing the amount of heat produced or changes in temperature in general (since cellular respiration is exergonic.)
Purpose The purpose of this experiment was to evaluate the stoichiometric relationship between the testing agents and to identify the products formed. The relationship was found by completing three acid and base neutralization reactions using phosphoric acid, which is a triprotic acid, with different volumes of sodium hydroxide. Introduction Procedure Phosphoric acid solution with a volume of 1.00 mL and a molarity of 6.00 M was transferred into a 125-mL Erlenmeyer flask using a volumetric pipette. Sodium hydroxide solution with a volume of 6.00 mL and a molarity of 3.00 M was transferred into a 50 mL beaker using a volumetric pipette. While swirling the phosphoric acid solution in the Erlenmeyer flask, the sodium hydroxide solution was added to it a few drops at a time using a disposable plastic pipette.
The main purpose for this experiment was to become familiar with the differences of elements, mixtures and compounds. The lab consisted on performing four different tests on both Iron (Fe) and Sulfur (S). If the four experiments were performed on each element then their would be a different reaction for each. The first test consisted of simply laying out a certain amount of each element of a piece of paper and observing the reaction when a magnet was placed on the bottom. The second test consisted on mixing in a 100ml beaker, 30ml of water and the respective amount used in the previous step of each element and analysing what the reaction was.