Guava Research Paper

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Present time medicinal plants being the effective source of medicines, either it can be modern or traditional medicines, the advantage of medicines are they are useful for health. Guava contains broad spectrum of phytochemicals including polysaccharides, vitamins, essential oils, minerals, enzymes, proteins, sesquiterpenoid alcohols, triterpenoid acids, alkaloids, glycosides, steroids, flavanoids, tannins, saponins. Psidium guajava linn or guava is very rich in antioxidants and vitamins and also high in lutein, zeaxanthine and lycopene. Guava is rich in phenols, triterpenes, carotenoids, lectins, fiber, fatty acids, quercetin, anthraquinones, phlobatannins and cardiac glycosides [1]. Much of guava therapeutic activity was attributed to these…show more content…
Kashibai Navale college Of Pharmacy, Kondhwa Bk. Preparation of Leave Extract
50 g of Psidium guajava linn leaves are washed with distilled water and keep in dark room for 4 days and grind into powder form. 2 g of powder dissolved in ethanol, methanol, pet ether of 50 ml of solvent respectively in incubator at 40oc for 5 days. The extract was subsequently filtered and concentrated to dryness.

Antibacterial assay
The screening was done by disc diffusion method. The extracts were tested against Escherichia coli. A loopful of the pure bacterial culture was suspended in nutrient broth and incubated for 24 hours. Nutrient agar media was sterilized and poured into plates. After solidification, 0.1ml of the inoculum was spread over the agar evenly using L rod. 6mm diameter of Whatman filter paper discs were soaked in plant extracts and dried out. Chloramphenicol and tetracycline antibiotic discs were used as the control. The discs were carefully placed on the inoculated plates and incubated for 24 hours. Later, the zone of inhibition around the disc was measured and
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The antifungal property of the gel was estimated by measuring the growth of fungi after 3 days of incubation. In the control plate, the fungi grew over the entire plate (9cm) in 3 days. Methanol extract showed excellent antifungal activity against nestatin, i.e. it exhibited only 13mm of growth in 3 days. Ethanol and petroleum ether extract also showed commendable results. Methanol extract showed good activity against nestatin, followed by ethanol and petroleum ether extracts. This study shows that methanol is the best solvent for extraction followed by ethanol and petroleum ether. The data summarized in table 2 shows that guava leaf is an exceptional antifungal

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