Present time medicinal plants being the effective source of medicines, either it can be modern or traditional medicines, the advantage of medicines are they are useful for health. Guava contains broad spectrum of phytochemicals including polysaccharides, vitamins, essential oils, minerals, enzymes, proteins, sesquiterpenoid alcohols, triterpenoid acids, alkaloids, glycosides, steroids, flavanoids, tannins, saponins. Psidium guajava linn or guava is very rich in antioxidants and vitamins and also high in lutein, zeaxanthine and lycopene. Guava is rich in phenols, triterpenes, carotenoids, lectins, fiber, fatty acids, quercetin, anthraquinones, phlobatannins and cardiac glycosides [1]. Much of guava therapeutic activity was attributed to these …show more content…
Kashibai Navale college Of Pharmacy, Kondhwa Bk. Preparation of Leave Extract
50 g of Psidium guajava linn leaves are washed with distilled water and keep in dark room for 4 days and grind into powder form. 2 g of powder dissolved in ethanol, methanol, pet ether of 50 ml of solvent respectively in incubator at 40oc for 5 days. The extract was subsequently filtered and concentrated to dryness.
Antibacterial assay
The screening was done by disc diffusion method. The extracts were tested against Escherichia coli. A loopful of the pure bacterial culture was suspended in nutrient broth and incubated for 24 hours. Nutrient agar media was sterilized and poured into plates. After solidification, 0.1ml of the inoculum was spread over the agar evenly using L rod. 6mm diameter of Whatman filter paper discs were soaked in plant extracts and dried out. Chloramphenicol and tetracycline antibiotic discs were used as the control. The discs were carefully placed on the inoculated plates and incubated for 24 hours. Later, the zone of inhibition around the disc was measured and
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The antifungal property of the gel was estimated by measuring the growth of fungi after 3 days of incubation. In the control plate, the fungi grew over the entire plate (9cm) in 3 days. Methanol extract showed excellent antifungal activity against nestatin, i.e. it exhibited only 13mm of growth in 3 days. Ethanol and petroleum ether extract also showed commendable results. Methanol extract showed good activity against nestatin, followed by ethanol and petroleum ether extracts. This study shows that methanol is the best solvent for extraction followed by ethanol and petroleum ether. The data summarized in table 2 shows that guava leaf is an exceptional antifungal
Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided
Gorki Aguila is a well known Cuban rock musician and leader of the Pomo para Ricardo band. Instead of just enjoying the life of a popular musician, Aguila is a vocal critic of Fidel Castro and uses his music to criticize the Cuban government. As a consequence, he has been banned from public or internet performances and has been arrested numerous times. According to a Fox News web page at http://latino.foxnews.com/latino/entertainment/2015/08/13/human-rights-group-condemns-government-threats-against-cuban-rock-band-leader/, I have learned that Aguila was arrested by the Cuban government as recently as early August for his support of the dissident group, The Ladies in White.
The Herpailurus yagouaroundi also known as Jaguarundi is apart of the kingdom animalia, phylum chordata, class mammalia, order carnivora, and family felidae. The Herpailurus yagouaroundi is one of the more unusual New World cat species (Jaguarundi videos, photos, and facts). It is somewhat weasel like in appearance, it is long and slender with short legs and a long flattened head, it also has a long tail. It can range in length from 28-54 inches from nose to the tip of the tail. Its coat lacks spots like other South American cats.
1. Rumspringa is Pennsylvanian Dutch for “running around”. Rumspringa starts when the Amish are between 14-16 years old, and ends when they have found a spouse. They're, in their Rumspringa period, allowed to do non-Amish things, for an example, they're allowed to drive cars and several non-Christian things. Neither do they have to pray, nor do they wear the traditional Amish clothes.
For far too long the voice of the voiceless has been suppressed by those who are in power, the Gachupines. The revolt in Guanajuato in the Alhondiga proved to be a turning point because it showed that when the “pueblo” is united it will never be defeated. For instance, about 20,000 to 25,000 supporters of Miguel Hidalgo de Costilla were beside him in the siege of the Alhondiga de Granaditas on September 28, 1810. Hence, his strong movement that is quickly gaining a lot of support is not a just a quest for independence, but it is a social and economic war against those who are in power, the Gachupines. They keep overexploiting our resources to their advantage at the expense of our country.
September 26, 1942 marked a riveting day for the literary world who received a new writing giant that would make a resounding impact for the Latino community for decades to come. Anzaldúa was born to parents Urbano and Amalia Anzaldúa and was the eldest of four children, all born in Raymondville, Texas. Anzaldúa spent her childhood living in ranches and even traveled to West Texas to become a migrant worker. At age 11, her family relocated to Hargill, Texas for better educational opportunities and through that, Anzaldúa’s vast knowledge of the South Texas landscape and experiences with discrimination soon came to influenced many of her later works. Anzaldúa became interested in writing at a young age, she wrote poetry, journal entries and
haramba is a god to us. he was a god for everyone. why did they kill him? is it because he 's a gorilla?haramba is a god to us. he was a god for everyone.
Since ancient times Hmong people as been around. So what is Hmong? Are they Chinese or Mongolian? Most people think Hmong are either Chinese or Mongolia For example the word Hmong is very similar to the word Mong in Mongolian.
Herbal products have been used for treatments since ancient times, before the exploration of synthetic industrial drugs. Since herbs are natural, most of the consumers believes herbal products are completely safe and the product use continuously increased and is reported 10-19% growth in United States. In U.S.A any botanical product affects the structure and functions of the body belongs to either a drug or dietary supplement and FDA regulates the dietary supplement. The author of this paper selected St. John’s wort ,as the herbal supplement for this assignment of patient educational flyer. Product Description: St. John’s wort (SJW) is a yellow flowering plant, grows in the wild used for health purpose for long time .The
The B. Vulgaris samples were approximately 1cm3. They were kept the same size to ensure accurate results. A control test was conducted in distilled water to obtain a result to compare. The ethanol treatments were 40% and 70%. To prepare the solutions a 70% ethanol solution was used to make 40%.
The sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used. The plate was incubate with lid up on the bookshelf at room temperature for 48 hours.
The nation of Brazil has given the world many dribbling magicians over the years, but none were as beloved or fondly remembered as Mané Garrincha. It 's easy to understand the masses ' infatuation with him – Garrincha 's tale is a story of a man who overcame multiple birth defects to become one of football 's greatest stars. With him as the team 's heart, Brazil won two consecutive World Cups in 1958 and 1962. In 1999, Garrincha was voted the seventh greatest football player of the 20th century. Having been born with a deformed spine and a right leg that was six centimeters longer than the left one, Garrincha 's future as a professional football player seemed rather bleak from the outset.
The media used in this experiment was Trypticase nitrate broth. The reagents used (A and B) were sulfanilic acid and alpha-naphthylamine (respectively). Using aseptic technique, the bacterium (16A and 16B) were inoculated into labeled broth test tubes. The tubes were incubated for 48 hours at 37 degrees Celsius. When the incubation was complete 5 drops of reagent A and 5 drops of reagent B were added to each of the broths.