Advantages Of Organ Culture

Topic name Organ culture

Name HASNAIN AHMAD KHAN Registration # 682-FBAS/BSBT/S15 Department BT and BI Class BS Biotechnology Submitted to Dr. Ali Imran Bukhari

ABSTRACT

Organ culture

INTRODUCTION
Organ culture is the growth or maintenance of the tissue organ primordial , the part or whole of an organ in vitro. In other words we can say that it is development from tissue

Cultured organs retains its physiological features e.g hormone dependent organ continue to be dependent.
Outgrowth of isolated cells from periphery of explants can be minimized by manipulating the culture techniques
Advantages
Explant are comparable to in vivo organs in both structure and function.
It provides information on the pattern of growth, differentiation and development of an organ.
In some cases it can also replace whole animals in experimentation as the results from are easier to interpret.
Limitations
Results from organ cultures are often not comparable to those from whole animal studies e.g. in studies on drug action the drugs are only metabolized in vivo not in vitro. Survey of organ culture technique
Loeb was first scientist to isolate the fragments of adult rabbit liver, kidney, thyroid and ovary on the small plasma clots inside a test tube and found that they retained their structure for 3 days
Loeb and Fleisher
They showed that the oxygen must be filled in tube to prevent the explants from central

This technique was first used by Spratt, Wolff and Haffen modified Gaillard’s Technique and used an ager gel contained in an embryological watch glass.
• The medium consists of suitable salts solution, serum, chick embryo extract or a mixture of
• Certain amino acids and vitamins. These are all gelled with 1 % agar solution
• This method avoids the immersion of explants into the medium and permits the use of defined media. Generally the explants need to be subcultured on fresh agar gels every 5-7 days. The agar gel are kept in embryological watch glasses and sealed with the paraffin wax. This method is used to study many development aspects of organs and also tumors. 3. Raft Method
Definition
The explants are placed on the raft of lens paper or rayon acetate which is floated on a serum in a watch glass. Rayon acetate rafts were made to float on the serum by treating their four corners by silicone. The floatability of lens paper is enhanced by treating them with silicone on each raft 4 or more explants are placed. In the combination of raft or clot techniques the explants are first placed on a suitable raft then it is kept on plasma clot. This modifications makes media changes easy and prevents sinking into the liquefied