Advantages Of Proteomics
The term “proteome” or “proteomics” was first introduced in 1995. Proteomics is the characterization and identification of all proteins that expressed by a genome or tissue and understanding how these proteins function (Mohanty, 2005). Besides, the purpose of proteomics is not only to recognize all the proteins in a cell but also to generate a complete three-dimensional (3D) map of the cell indicating where the proteins are located. To achieve these goals require the involvement of a large number of different fields such as biochemistry, molecular biology and bioinformatics. Many different areas of study are now grouped under the proteomic such as sequence and structural proteomics, expression proteomics, interaction proteomics and functional …show more content…
This 2-DE method is relatively simple visual for mapping differences in protein expression. There are some disadvantages by using this technology such as poor solubility of membrane proteins, low detection sensitivity and linearity, limited loading capacity of gradient pH strips, low throughput, and reproducibility of gels and also low linear range of visualization procedure. However, this method shows the fastest method for direct targeting of protein expression differences (Monteoliva & Albar, 2004).
Next is interaction proteomics. Protein-protein interactions (PPIs) are important for all biological processes. Besides, collecting PPI networks provides many new insights into the protein function (Stelzl et al., 2005). It is estimated that over 80% of the proteins operates in a complexes and these PPIs are regulated by several mechanisms that act synergistically. Protein complex is the dynamic entities that assemble in an ordered time (Volkel et al.,
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Functional proteomic is a process which used proteomic method to monitor and analyse the spatial and temporal properties of the molecular networks and fluxes involved in living things (Monti et al., 2005). In certain cases, specific subproteomes are isolated by using affinity chromatography and might include the isolation of protein complexes or the protein ligands that used to isolate specific types of proteins. This method allows a selected group of proteins to be analyse and characterized that can provide important information about disease mechanism, protein signaling or protein-drug interaction (Graves & Haystead,
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