The Agroinfiltration Method
Transient transformation
The transient production platform is perhaps the fastest and the most convenient production platform for plant molecular farming (Rybicki, 2010). This can be achieved through following methods,
(A) Agroinfiltration method
The agroinfiltration method, which was developed by Kapila et al. (1997), involves infiltration of a suspension of recombinant Agrobacterium tumefaciens into leaf tissue, which facilitates the transfer of T-DNA to a very high percentage of the cells, where it expresses the transgene at very high levels without stable transformation, as in the case of transgenic crops. This method has now been developed into a very rapid, high-yielding transient expression strategy for producing clinical grade bio-pharmaceuticals
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1996). Transient expression can be used in pilot experiments before proceeding to the time consuming and costly stable transforma¬tion. Problems in protein expression can be identi¬fied and corrected so that the chances of producing the desired protein through stable transformation are made more likely. Through agro-infiltration a number of genes can be expressed at the same time which can help in studying the combined effect of multiple transgenes which are expressed (Johansen & Carrington 2001). However, the yield of ex¬pressed protein is normally lower and the plant material requires processing immediately as due to the perishable nature of leaves degradation during the storage of plant tissue will result in further loss of the …show more content…
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