Gram-staining have performed for Staphylococcus aureus (control); Enterococcus faecalis (control); Nostril microflora in NA, MSA, and PYCa. Gram-staining provides results of the bacteria morphology, type of gram-stain. Catalase test was also done prior to gram-staining. MicrobactTM Biochemical Identification Kit was used for identifying gram-negative aerobic and facultatively anaerobic bacteria.
Eosin-methylene blue agar 4. MacConkey agar 5. KF Streptococcal agar • Divide the agar plates into quarters and name each quarter with the four different cultures provided which include: 1. Escherichia coli 2.
The first of the four test was a Gram-stain. Once the experiment was completed the slide was then placed under a microscope where it was then categorized as Gram-negative bacilli. Its pink color when viewed through the microscope indicated that the test was Gram-negative and its rod shape indicated the morphology was bacilli. With these results the next test to be conducted was the citrate utilization test. A citrate tube was inoculated with P. aeruginosa and incubated at 37 degrees Celsius at 24 hours.
Discovered in 1953 by Jacques Monod and colleagues, the trp operon in E.coli was the first repressible operon to be discovered. This operon contains five structural genes: trp E, trp D, trp C, trp B and trp A, which encodes tryptophan synthetase. It also contains a repressive
Public Health Reports (1896-1970), 29(43), 2821–2825. http://doi.org/10.2307/4571457 Jagielska, Gabriela, M.D., PhD, Tomaszewicz-libudzic, C., & Brzozowska, Agata, M.D., PhD. (2007). Pellagra: A rare complication of anorexia nervosa. European Child & Adolescent Psychiatry, 16(7), 417-20. doi:http://dx.doi.org/10.1007/s00787-007-0613-4 Park, Y. K., Sempos, C. T., Barton, C. N., Vanderveen, J. E., & Yetley, E. A. (2000).
The cellulose acetate membranes are cut in 40xl00 mm each and soaked in TEB buffer for 5 minutes. 5 strips are plotted then placed on the electrophoresis tanks. 4. Supply the voltage current at 250 V for 5 minutes to the membranes to equilibrate the membranes with the buffer. 5.
9) Schuelke M, Mayatepek E, Inter M, Becker M, Pfeiffer E, Speer A, et al. Treatment of ataxia in isolated vitamin E deficiency caused by alpha-tocopherol transfer protein deficiency. J Pediatr
Natalie Koch Bio 2 Metridium The metridium, which is also known as the plumose anemones, is a sea anemone that is classified under the kingdom Animalia and the phylum Cnidaria. Which is the same phylum as jellyfishes and corals. The metridium is typically found in cooler waters of the Pacific and Atlantic oceans up in the Northern Hemisphere (Wikipedia.com, 2013).
onvergence of Adaptive Noise Canceller '); legend( 'Measured Signal ', 'Error Signal '); subplot(3,3,6); plot(t,e, 'r '); hold on; plot(t,fhb, 'b '); axis([Time-4 Time -0.5 0.5]); grid on; xlabel( 'Time [sec] '); ylabel( 'Voltage [mV] '); title( 'Steady-State Error Signal '); legend( 'Calc Fetus ', 'Ref Fetus ECG '); filt_e = filter(Hd,e); subplot(3,3,7); plot(t,fhb, 'r '); hold on; plot(t,filt_e, 'b '); axis([Time-4 Time -0.5 0.5]); grid on; xlabel( 'Time [sec] '); ylabel( 'Voltage [mV] '); title( 'Filtered signal '); legend( 'Ref Fetus ', 'Filtered Fetus '); thresh = 4*mean(abs(filt_e))*ones(size(filt_e)); peak_e = (filt_e >= thresh); edge_e = (diff([0; peak_e]) >0); subplot(3,3,8); plot(t,filt_e, 'c '); hold on; plot(t,thresh, 'r '); plot(t,peak_e, 'b '); xlabel( 'Time [sec] '); ylabel( 'Voltage [mV] '); title( 'Peak detection '); legend( 'Filtered fetus ', 'Dyna thresh ', 'Peak marker ', 'Location ', 'SouthEast '); axis([Time-4 Time -0.5 0.5]); subplot(3,3,9); plot(t,filt_e, 'r '); hold on; plot(t,edge_e, 'b '); plot(0,0, 'w '); fetus_calc = round((60/length(edge_e(16001:end))*Fs) * sum(edge_e(16001:end))); fetus_bpm = [ 'Fetus Heart Rate = ' mat2str(fetus_calc)]; xlabel( 'Time [sec] '); ylabel( 'Voltage [mV] '); title( 'Reconstructed fetus
Once the gel hardened, .5X TBE (44.5 mM Tris base, 44.5 mM boric acid, and 1.0 mM EDTA) was added just until the gel was covered with the TBE buffer. Each sample was loaded into the gel as well as 10 μL of DNA size markers (1kb ladder, New England Biolabs) into a separate lane. The gel was allowed to harden at room temperature and then electrophoresed at 100 volts for 75 minutes. Using a UV imager, a photo was taken of the resulting traveled DNA fragments in the gel.