.4 Agar Disk Diffusion The antimicrobial tests were carried out according to disc diffusion tests (Lennette et al., 1985, Kim et al., 1995). Cells were streaked on MHA agar plate to obtain colonies at 37°C for 16 hrs after which they were resuspended in sterile saline solution to give an O.D of 0.1 at 600nm. On solidified 2% MHA plate, the bacterial culture is swabbed and sterile disc impression was made, 10 µl plant extracts was loaded on the agar plate. After 10 minutes the plate is incubated
Potato Dextrose Agar 1. 39.0 g of potato dextrose agar powder was dissolved in a litre of sterile distilled water on the hot plate. 2. pH of the solution was adjusted to 5.6 ± 0.2 by adding NaOH or HCl and was immediately transferred into the Schott bottle to be autoclaved at 121 ° C for 15 minutes 3. Prepared medium was stored in 4° C chiller Plate Count Agar or Total Plate Count 1. 22.5 g of plate count agar powder was dissolved in a litre of sterile distilled water on the hot
VIII. Methods and materials : hemoglobin types determined by cellulose acetate and citrate agar electrophoresis methods based on different electrical charges when place the sample in electrophoresis, when electrical current is passed through the hemoglobin blood sample, causes separation of hemoglobin type at different rates and form bands .(14)(15) materials 1. Tris-EDTA Boric Acid buffer (TEB), pH 8.4. Tris hydroxymethyl amino methane (TRIS): 10.2 g Ethylene diamine tetracetic acid (EDTA):
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain. These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements
EFFECT ON HUMAN BEINGS 1.1 Stenotrophomona-maltophilia is a gram negative, non-fermentive, aerobic bacteria which is usually found in aquatic environments and plant rhizospheres. It is motile due to the polar flagella and colonizes well in MacConkey agar, which is not a generally discovered pathogen among human beings. It is resistant to many drugs and it mainly affects the nosocomial region on individuals with low immunity. Though the organism can cause infections among various tissues, organs etc
author: Sri Santhi Lanka, E.mail srishanthichowdary@gmail.com Abstract: To improve the productivity of antibacterial compounds of Lactobacillus fermentum by optimizing its nutrient and physical factors and screened for its antimicrobial activity by agar well diffusion method. In order to improve its efficiency,
Packaging is part the end of a production process food or other products. Packaging useful to increase the consumer revenue, also reduce degrees damage during the transport of products. Packaging is also one of a way to protect or adding power savings food products and non-food. Packaging is not only aimed to preserve, but also be a means of supporting in transportation, distribution, and forms an important part of an effort to overcome competition product marketing. Packaging industry mostly dominated
Short title: Functional Characterization of Biosurfactant Functional Characterization of Biomedical Potential of Biosurfactant Produced by Lactobacillus helveticus 1, 2Deepansh Sharma, 1*Baljeet Singh Saharan and 3Shailly Kapil 1Department of Microbiology, Kurukshetra University, Kurukshetra 136 119 INDIA 2Department of Biotechnology and Biosciences, Lovely Professional University, Jalandhar 3Dairy Microbiology Division, National Dairy Research Institute, Karnal132 001 INDIA *Baljeet.kuk@gmail
Ingredients like sodium alginate, liquid nitrogen, calcium citrate, citric acid, lecithin and agar are often used. Even though chefs’ juggling around with the flavours in molecular gastronomy, these flavours are not actually lost rather they are enhanced by using various ingredients and methods. With the rise of global Indian, the Indian food has
condition favourable to their growth. 3.2MEDIA PREPARATION 3.2.1 For Bacteria: • Weigh 3.3 grams of CC-i (M tryptone Glucose Extract Agar) medium. • Dissolve in 1 OOml distilled water. • Cotton plug the flask. • Wrap cotton plug with Aluminium foil. • Sterilize by autoclave at 15 pounds (121°C) for 15 minutes 3.2.2 For Coliforms: • Weigh 6.3 grams of CC-3 (MF-Endo Agar) medium. • Dissolve in 100 ml of distilled water containing 2 ml ethanol. • Cotton plug the flask. • Wrap cotton plug with Aluminium