Animals were also weight immediately prior to sacrifice (fasted body weight). Animals were sacrificed under anesthesia with diethyl ether, and then blood samples were immediately collected in clean and dried Wiesserman tubes from the portal vein. First part of blood was collected in tubes containing potassium oxalate and sodium fluoride for the estimation of plasma glucose by O-toluidine method of Sasaki et al. (1972). Second part of blood was left to coagulate then centrifuged at 3000 rpm for 15 minutes to obtain serum to estimate some biochemical parameters.
Abstract: The present study investigated the anxiolytic, antidepressant and antistress actions of the aqueous extract of Cinnamomum tamala (CT) leaves in rats. CT (100, 200 and 400 mg/kg) was administered orally once daily for 7 days and the results were compared by those elicited by lorazepam (1 mg/kg, p.o), imipramine (10 mg/kg, p.o),W.sominfera (100 mg/kg, p.o) for anxiolytic, antidepressant and antistress studies respectively. All these standard drugs were administered once, 30 min prior to the tests. Results were analyzed by One-way ANOVA followed by Newman-Keuls Multiple Comparison Test. P<0.05 were considered significant as compared to control.
Hypothesis: If one-day pinto bean seedlings are soaked in a water solution, 1% NaCl solution, and 3% NaCl solution, the seedlings exposed to higher sodium concentrations will have decreased cellular respiration rates. Treatments: This experiment involved three treatments and a control. The goal of the experiment was to see how different levels of NaCl affect the rates of cellular respiration in day-old pinto bean seedlings. In this experiment the rate of cellular respiration was measured by the amount of CO2 in ppm per gram of substance produced by a given treatment group or the control over the course of ten minutes. CO2 levels were measured using a CO2 sensor.
Echocardiography, non-invasive blood pressure measurements, collection and analysis of blood and plasma, tissue analysis, and statistical analysis were all the methods used to perform this experiment. Every experimental group contained saline or isoproterenol and was inserted below the skin and back. Gene expression levels were also measured at 3 and 14 days after the infusion for the cardiomyocyte injury. During the echocardiography, the measurements needed were traced by the levels of the papillary muscles. For the non-invasive blood pressure measurements, systolic and diastolic blood pressure were measured on the mice’s tail.
In order to accurately determine the amount of dosage to be administered to each animal, the rats were all weighed one hour prior to the experiment. I ran a total of twelve groups in the Open Field Apparatus with each group running two rats each . The experimental rats were administered an IP injection using a 2% dosage of LiCl solution at 85 mgs/kg. Control groups were given a baseline injection of NaCl. The Open Field Apparatus was placed over 48 12x12 tiles in a dimly lit room .
The sunflower seed is the seed of the sunflower (Helianthus annuus). The methanol extract of seeds of Helianthus annuus were screened for analgesic activity in mice model to systematically explore the medicinal values of the plant. Acetic acid induced writhing and hot plate methods were used to confirm the central and peripheral analgesic action. The extract at dose 100mg/kg and 200mg/kg showed attenuated writhing inhibition at 50.35% and 57.85% in case of acetic acid-induced writhing test which showed significant analgesic activity (p< 0.05). In the hot plate method increase (p < 0.05) of latency period was also observed in comparison to standard aspirin.
About 26,000 children are being hospitalized each year due to tooth decay. Type 2 diabetes is a chronic condition that affects the way of the body processes blood glucose(sugar). Although sugar does not cause diabetes, it can create the body to gain weight. Being overweight can increase the chances of developing type 2 diabetes(Article Diabetes Myth). Research has shown that sugary food and drinks link to type 2 diabetes because it causes weight problems.
It is the most common type of diabetes with 90-95% of the cases of diabetes resulting in type 2. Type 2 diabetes causes the the pancreas to create too much insulin which causes insulin resistance. Because of insulin resistance the levels of sugar in the bloodstream rises and causes the red blood cells to become coarse which damages the circulatory system. The thrifty gene theory is metabolic abnormality that creates juvenile diabetes in the feotus. it was theorised to be traced back to homo sapiens whom starved during famines.
The tube was placed back in incubation for 96 more hours to observe any more positives. 2.10 Catalase Test A trypticase soy agar plate was used and after incubation, four drops of 3% Hydrogen Peroxide was added to the plate to flow over the bacterial growth. A presence of bubbling was observed. 2.11 Starch Hydrolysis A starch agar plate was inoculated with a streak of the unknown bacteria and then incubated. On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids.
Sugary urine has historically been known as a symptom of diabetes. The author writes, “In the past Chinese physicians actually diagnosed and monitored diabetes by looking to see whether ants were attracted to someone’s urine.” Therefore, sugary urine was a key factor for detecting and treating of the disease in the past. Today’s treatment typically consists of medication, exercise, and a balanced diet. Unfortunately, diabetes has had a huge impact on humanity, as it currently affects about 171 million people
After identifying reagents and finding the theoretic yield, it’s possible to find the excess reagent mass and number of moles for each test. To do this, the smallest mole number of Ca(OH)2 was subtracted from the highest one. The result is used to find the amount of moles excess, by multiplying it to the corresponding number of moles of excess reagent and dividing then by 1 mole of Ca(OH)2. After finding the answer in moles, it’s possible to find the number of grams by following the rules of conversion factors from moles to grams. 5.
In 2005, researchers at the Ramazzini Foundation in Bologna, Italy conducted such a study. The study found that rats exposed to aspartame starting at 8 weeks of age, and continuing throughout their lives, developed leukemias, lymphomas, and kidney tumors. In 2007, the same researchers published a follow-up study that exposed rats to aspartame beginning in the womb and continuing throughout their lives. It found that aspartame caused lymphomas, in addition to mammary (breast) cancers. In 2010, the group exposed test mice to aspartame while they were still in the womb again, and made the startling discovery that the mice developed liver cancers and lung cancers
In fact, through a recent study at Princeton University, after observing changes in rats that consumed high fructose corn syrup, scientists were able to conclude that the cheap sugar substitute has addicting effects on the brain “similar to some drugs of abuse” (Parker, 2010). Behaviors that individuals who take in excess amounts of HFCS encounter such as binge eating, withdrawal, and craving, for instance, all relate to the same neurochemical change, where enkephalin mRNA, dopamine, and acetylcholine release in the nucleus accumbens, in the brain that occur in humans addicted to drugs (Avena, 2007). Thus, in the same way that a drug fiend depends on drugs humans begin to depend on High Fructose Corn Syrup, both needing their daily fix which is especially apparent in the growing number of overweight people since HFCS became present in nearly every processed food (Avena,
In place of the bubonic plague, though, the pathological agents in obesity and type 2 diabetes are identified as “high-fat and high-calorie diets,” and instead of fleas and rodents, the causes are “advertisements and inducements to poor lifestyle” (3). More than twenty illion Americans are currently diagnosed with diabetes, a tripling of cases since 1990 C. Diabetes
In September 2004, a 17 year old girl was diagnosed with Type 1 Diabetes Mellitus in Craigavon Area Hospital. She presented with key symptoms of hyperglycaemia, tiredness, blurred vision and an increased thirst and polyuria. She also displayed signs and symptoms of ketoacidosis at the time of diagnosis. HBA1C analysis and glucose test results also supported the diagnosis. She was diagnosed with Type 1 diabetes mellitus and began treatment management of her glucose levels by administration of frequent insulin injections throughout the day on a basal bolus regime.