The purpose of this experiment is to create a complete genomic library of Aliivibrio fisheri through the use of the lux operon. The examination of the lux operon gene occurs through the extraction of the DNA of Aliivibrio fischeri and digest a large piece of DNA to smaller random pieces. The fragment of DNA will later be ligated together in plasmid. Plasmid acts as vectors to transport DNA from one organism to another. The DNA will then run through a UV-visible spectrophotometer to test the absorbance of the extracted DNA. Both DNA and RNA has a maximum absorbance of 260 nm. The absorbance of 260/280 should be in between 1.8 and 1.9 to represent a pure sample of DNA. If the reading is higher than 1.9 then there is RNA contamination and if the reading is less than 1.8 there is protein contamination. …show more content…
Since the production of light requires a large amount of energy expenditure, Vibrio fischeri uses quorum sensing to regulate its gene expression after detecting changes in extracellular density. Quorum sensing is used by both Gram-positive and Gram-negative bacterium . Lux R and lux I are genes encoding proteins that regulates light production.Aliivibrio fischeri also forms a symbiotic relationship with animal hosts. Aliivibrio fischeri utilizes the nutrients provided by its host to emit light that is later used by the host for various purposes. The light emitting reaction of Aliivibrio fischeri is catalyzed by
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Joyce Balingit BIOL 196 - 1006 Instructor: Austin McKenna 30 October 2015 Writing Assignment #3 Part A In meiosis, the cell goes through the same stages as mitosis twice. The stages of Meiosis I are: prophase I, metaphase I, anaphase I, and telophase I.
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
Elijah Brycth B. Jarlos IX-Argon 1. Multicellularity is a condition of an organism to have multicellular cells. An example of a organism who has multicellular cells are plants, animals, and humans. The main reason of why scientists have a hard time finding a good set of existing organisms to compare. Is neither the first set of organisms which is being compared is dying as fast as the second specimen is being examined or they just can’t find the right species.
These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where. A number of tests can be ran on an unknown bacteria to determine their ideal
I don't accept my current grade, because it is wrong, and my grade should be higher than D in anyhow according to Dr.Scandale's grading policy. The following explanation is made on my behalf. Lab 1.1 grade = 0 "what should be the correct grade?", and if different, explain why
The Unknown Identification Lab was an experiment that provided the opportunity to apply all the tests that were learned in the semester of lab, to identify the two bacterias that remain unknown. Gram- staining and two other tests will be used to identify the unknowns. This experiment is crucial to the understanding of each test, and can benefit in the ability to identify the characteristics of specific bacteria. Having a clearer understanding of the bacteria can further the research of bacteria for medicine, such as antibiotics. The understanding can also help the development of research in the environment.
The membrane permeability of Beta vulgaris is affected by ethanol solution Introduction Cell membranes are the semi-permeable membrane that surrounds all cells. It separates the extracellular environment from the intercellular environment. It is a phospholipid bilayer which contains various proteins, lipids and carbohydrates all serving different purposes. It is this structure which allows for the transport of nutrients, proteins and water. (Nature.com, 2014).
Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
chinesis. A construct of R751::Tn4351 (the physical map of R751::Tn4351 and restriction sites are shown in fig. 7) was selected for introduction into F. chinesis to discover if the introduction and insertion of the vector R751 and the transposition of T4351 into the F. chinesis chromosome by a triparental mating occurred. One parent was E. coli GJ342 which carried a helper plasmid, the second parent was E. coli HB101 which contained R751::Tn4351 and the third parent was the F. chinesis target strain. 189 colonies were isolated on LB agar plates which in passage in fresh media were able to grow in 200µgml-1 erythromycin.
Introduction Our world is composed of many bacteria’s’ that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods that have been learned in the laboratory to determine our unknown bacteria. Bacteria’s can have different features, shapes, and or arrangements that help microbiologist determined their role in our life (whether they are good or bad for humans).
All of these components are used in a method called flux balance analysis (FBA) to simulate microbial metabolism in a specified environmental condition. All of these components are used in a method called flux balance analysis (FBA) to simulate microbial metabolism in a specified environmental condition. FBA involves the use of linear optimization to define the limits on the metabolic capabilities of a model organism by assuming that the interior of the cell exists in a quasi-steady-state. These mass balance constraints and reaction flux bounds form a set of underdetermined linear equations with many possible solutions. Maximum growth yield is simulated by maximizing the flux through the biomass reaction in the model, while the uptake of nutrients is fixed at a specific ratio.
INTRODUCTION: Arginase is an enzyme- enzymes are biological catalyst which drives a reaction at the speed of life. Arginase is a hydrolase, hydrolases catalyze hydrolysis reactions, this is determined via the E.C number (Nelson and Cox 2008). Arginase has the EC number is 18.104.22.168 (Schomburg 2015). The enzyme ‘commission number’ is the arithmetical classification that is used for enzymes which indicates the chemical reaction they catalyze.
The streaking technique used was a modified streaking for isolation with a heavy quadrant one. The result revealed that bacteria is alpha, with an incomplete breakdown of the medium with a susceptibility of 17mm from the bacitracin gamma hemolysis. That is why the organism represented by the bar graph was in low numbers because it was incomplete. The other test was DNase agar, it is an enzyme test used to identify if the organism has the enzyme DNA. The streaking technique is a single straight line down the middle of the plate.
Prokaryotic organisms normally have a cytoplasmic membrane, cell wall, and sometimes a capsule. Bacterial cells are most commonly either coccus or bacillus in shape. The cell wall is either Gram positive or Gram negative. When the cell is Gram negative, the cell has an extra layer of lipopolysaccharides. The Gram positive has a thick layer of peptidoglycan.
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.