In order to synthesize this compound, measure out 1 gram of NaC2H3O2.Weighed 1 gram of NaC2H3O2 and mixed it with ionized water. Boiled 12 mL of 1.0M Acetic Acid added into a beaker containing the sodium carbonate on a hot plate until all the liquid is evaporated leaving solid precipitate form inside of the beaker. Weigh the precipitate form and mix it with water until fully dissolved. Weigh the precipitate remaining. A flame test and a halide ion test were performed.
Each 0.1M Sodium hydroxide solution that had been rinsed was drain into the waste container located under the hood. 3. The burette was filled with 0.1M Sodium hydroxide solution(prepared prior of this experiment) to 50 ml volume and the burette was clamp vertically(the air bubbles was remove from the tip of the burette by draining the 0.1M Sodium hydroxide into smaller beaker) 4. The 10g or 10ml amount of samples was inserted into 250ml conical flask and with addition of 50ml distilled water 5. Three to five drops of phenolphthalein were added into conical flask 6.
3.2.1 Ferric Chloride Test The sample extraction will be dissolved in 2 ml ethanol. A few drops of 10% ferric chloride solution will be added in test tube F. A green-blue coloration indicates the presence of a phenolic hydroxyl group (Bhatt et. al., 2011) 3.2.2 Sodium Hydroxide Test Few drops of 10% aqueous sodium hydroxide solution will be added in a test tube E with 2-3 ml of the extract. Formation of intense yellow color that became colorless on the addition of few drops of diluted HCl indicates the presence of flavonoids (Bello et. al.,
The eggs were divided into 5 groups, each treatment was done in triplicates. Calamansi crude extract was reconstituted to three concentrations: (Treatment 1, 99μ of distilled water and 1μL of Calamansi crude extract) 1%, (Treatment 2, 95μ of distilled water and 5μL of Calamansi crude extract) 5%, and (Treatment 3, 90μ of distilled water and 10μL of Calamansi crude extract) 10%. Retinoic acid was used as a positive control, and ethanol as negative control. Approximately, 100μL of each treatment was placed in the filter paper. After the introduction of test solutions, the eggs were sealed with a tape and incubated for
TLC method 1 Solvent system: Chloroform: methanol: 25% ammonia (8:2:0.5). Spots can be detected after spraying with Dragendorff reagent Orange spot (Mallikharjuna et al., 2007). 2) Anthraquinone Borntrager's test Heat about 50mg of extract with 1ml 10% ferric chloride solution and 1ml of concentrated hydrochloric acid. Cool the extract and filter. Shake the filtrate with equal amount of diethyl ether.
NOTE: Record the grams of gasoline, kerosene, and lubricating oils that are present in the 50 mL of crude oil. ➢ Select the flask, and choose Heating Mantel option afterward select Max Heat and make sure you record the temperature when you see crude oil begins to boil. ➢ When the crude oil begins to boil, Make sure you turn the temperature down to 60% by decreasing the heating metal two times. Step 3: Record the
Standardization of NaOH solution The prepared solution in part A was used to determine the acidity of the two different brands of soft drinks. But before it, the NaOH solution was standardized first. A 0.15 g of potassium acid phthalate was dissolved in 0.05 L of water in an Erlenmeyer flask. Afterwards, 3 drops of phenolphthalein was added. A 50 mL buret was obtained and was washed with NaOH solution.
The buret is filled to a point above the "0" mL mark with NaOH solution. In order to fill the tip of the buret with liquid, the solution is drained out of the bottom until the meniscus lies between the "0" and "1" mL marks. The initial buret reading can now be recorded to the nearest 0.01 mL. If you have any doubts as to your ability to read the buret correctly, ask your instructor to check your initial reading. Standardization of NaOH solution Accurately weigh out a sample of approximately 0.3-0.4 g of primary standard potassium hydrogen phthalate, KHPh, which has been previously dried at 120°C.
Then, a drop of concentrated sulphuric acid was added. The appearance of purple colour, which rapidly changed to violet, indicated the presence of resins (Cuilel, 1994). Test for tannins 2g of both extracts were weighed and placed in test tubes. Two drops of 5% ferric chloride solution was added into the test tubes. The appearance of dark green colour indicated the presence of tannins (Cuilel, 1994).
Stage 1: Sampling The samples are cycled over the MEPS Barrel insert and needle (BINS) so as to transfer the interested compound into the sorbent. Stage 2: Washing 20 µL to 50 µL of washing fluids are cycling over the BINS in order to withdrawn the non-specific binding compounds. Stage 3: Elution The interested compounds are desorbed by introducing the elution solvent into the BINS. Stage 4: Injection Finally, the samples are injected into the liquid or gas chromatograpy. Advantages of MEPS 1) Little amount of sample volumes is required up to 10