Table: 2 1. Chemicals used: Sl.No CHEMICAL GRADE SUPPLIER 1. ETHANOL AR ALDRICH 2. HEXANE LR MERK 3. ACETONE LR SD FINE 4. SILICA GEL LR SISCO 5. HYDROCHLORIC ACID LR SD FINE 6. METHANOL LR SD FINE 7. CHLOROFORM LR SD FINE 8. MORQUIES REAGENT AR SD FINE 9. MAYERS REAGENT AR MERK 10. DRAGENDORFFS REAGENT AR SD FINE 11. PICRIC ACID AR ALDRICH 12. POTTASSIUM IODIDE AR ALDRICH 13. AMMONIA AR ALDRICH 14. BROMINE WATER AR ALDRICH The following tests were carried out. Tests for alkaloids a. Morquies test: For detecting the alkaloids 2-3 gms of the sample was ground with sufficient chloroform to make slurry. Ammonical chloroform was added and the mixture was stirred for one minute. Extraction of alkaloids from choloroform was accomplished by shaking …show more content…
2 ml of drug solution was added with the reagent and colour was observed. Appearance of reddish brown precipitate showed the presence of alkaloids. e. Wagners test: Wagners reagent is a solution of Kl3 in water. It was prepared by dissolving 1.3 gm of I2 in a solution of Kl (2 gm) in water and made in to 100 ml. 2 ml of drug solution was added with the reagent and colour was observed Red coloured precipitate showed the presence of alkaloids. Test for Quinine (Bromine - ammonia test) To about 10 ml of (1 gm in 1000) solution of sample was added with 0.25 ml of Br2/H2O and shaken well. Then about 2 ml of dil.NH3 solution was added. No bright colouration showed the absence of quinine. Test for Morphine (Iodic acid test) Morphine liberates iodine from iodic acid which gives blue colouration. 2 ml of sample solution, acidified with sulphuric acid was added to a solution of KlO3 containing starch. Absence of deep blue colouration. Test for Terpenoids (Leibermann Buchard test): 2 ml of drug solution was dissolved in chloroform and to these 2 drops of acetic anhydride was added and concentrated sulphuric acid was added along the sides of the test tube and the colour was observed. Appearance of red colour indicated the presence of …show more content…
The mixture was allowed to stand for half an hour. Then 5 ml of hydrochloric acid and 5 ml of nitric acid was added and allowed to stand for 1 hour. The content was evaporated on hot plate to dryness. The excess of iodine was removed by adding hydrochloric acid. The residue was dissolved in boiling water and 15 ml of 25 % Ba Cl2 was added. Then allowed to stand for overnight. The precipitate was filtered through Whatman no 41 filter paper. The filter paper with residue was kept in a pre-weighed crucible and ignited in a muffle furnace. From the weight of the residue, the percentage of sulphur in the drug was
It is soluble in water and N,N-dimethyl formamide; slightly soluble in methanol; very slightly soluble in ethanol, acetone, and acetonitrile; and insoluble in isopropanol and isopropyl
Last test, inoculation of phenylalanine agar is used to determine if phenylalanine deaminase oxidizes phenylalanine into phenylpyruvic acid and ammonia. Sixth test, is a Multiple Test Media used to determine the physiological characteristics of unknown #398. First test, Inoculation of Kligler 's Iron agar was used to determine the production of hydrogen sulfide from cysteine and fermentation of glucose and lactose. Last test, inoculation of litmus milk is used to determine the fermentation of lactose, casein, lactalbumin, and
Next, about 10 mL of both solutions, Red 40 and Blue 1, were added to a small beaker. The concentration of the stock solution were recorded, 52.1 ppm for Red 40 and 16.6 ppm for Blue 1. Then, using the volumetric pipette, 5 mL of each solution was transferred into a 10 mL volumetric flask, labelled either R1 or B1. Deionized water was added into the flask using a pipette until the solution level reached a line which indicated 10 mL. A cap for the flask was inserted and the flask was invented a few times to completely mix the solution. Then, the volumetric pipette was rinsed with fresh deionized water and
Since it is also a solvent, it has many cleaning uses. We predicted at once that is could be isopropanol because of its smell. Question: “What is the identity of unknown 6 in test tube 20?” Hypothesis: If I test the characteristic properties of unknown 6 then I will see its properties to be the same or similar to isopropanol.
Identification of an Unknown Compound using Quantitative and Qualitative Analysis Lauren Tremaglio Chemistry 1011 Lab, Section 16 Instructor: Steven Belina October 3, 2014 Our signatures indicate that this document represents the work completed by our group this semester. Experimental Design and Discussion of Results The objective of this experiment was to identify an unknown compound through quantitative and qualitative analysis. In order to find the identity of the unknown compound, an initial qualitative test for solubility was performed.
To test for the presence of the ammonia cation, a scoop of the unknown compound was mixed with NaOH to see if the resulting solution had a noticeable smell that would affirm the presence of ammonia. A moist piece of pH paper was then held near the solution to see if the ammonium would dissolve the water on the pH paper. The next cation test performed was the flame test. A gram of the unknown compound was held by a metal stick above the flame of a Bunsen burner to see what color flame the compound would produce. The chart in the lab manual on page 63 was used to determine what cation correlated with what flame color.
On January 31, 2000, Dr. Harold Shipman was convicted of murdering 15 of his patients, as well as, forging a will of 1 of them. The evidence against him was that he had killed them by administering lethal doses of either morphine or diamorphine, more commonly known as heroin. Shipman had been convicted for fraudulently obtaining drugs in order to support an addiction when he was 29. As a result of his past addiction, the police considered that he had obtained heroin illicitly for his killings just as he had obtained the drugs 1975 to feed his addiction. Another way that Shipman may have obtained the drugs is that, he would issue prescriptions for patients, those who required the drug and those who did not, retaining some or all
Luminol 5. Introduction In this experiment, luminol was prepared from 3-nitrophthalic acid and hydrazine under high heat. 3-nitrophthalic acid and hydrazine produced the precipitate 3-nitrophthalhydrazine, which was isolated using vacuum filtration. 3-nitrophthalhydrazine reacts with sodium dithionite to produce luminol.
Results: Table 1: Results from the Salicylic acid (g) and the Purity of ASA (g) Experiment Mass of salicylic acid (g) 0.14 g Mass of filter paper (g) 0.47 g Mass of “impure” ASA + filter paper (g) 0.56 g Mass of prepared “impure” ASA (g)
Starch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.
These must be executed in focused laboratories to measure the receptiveness to antimalarial compounds of parasites collected from a definite patient. Two main laboratory methods are available: In vitro tests: where the parasites are grown in culture in the presence of increasing concentrations of drugs; the drug concentration that inhibits parasite growth is used as
The only part that was irrelevant was the appearance of the drug. All but one drug looked like white powder. Even white medicines are dyes to look white (“Toxins in Vitamins and Medicine”). The appearance of the drug therefore does not help us identify what drug the Unknown drugs are. Our conclusion is irrefutable because Unknown A was the same as Excedrin in every category we tested and the same goes for Unknown B and bufferin.
In this lab, we tested 8 known ingredients to find what ingredients was in our unknown A and unknown B medications. We first tested the water solubility of our knowns and unknowns. We found that of the knowns, cornstarch and acetaminophen were the only ones not water soluble. The unknowns were also not water soluble. Th next test was the pH test.
3. To purify and identify the product, recrystallization is used in order to purify the product, then melting point and TLC techniques are used to identify the product. Theory 4.
The hydrogen atom of –NH2 group is replaced by the acyl group. The crude product contained acetanilide, and acetic acid, which was the impurity. Recrystallization, which was used to purify the contaminated acetanilide, required a suitable hot solvent which dissolved all the crude product, but upon being cooled, since