Ampicillin Lab Report

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In this experiment, Tetracycline and Ampicillin were used to test for the antibiotic resistance of Pseudomonas fluorescens, lab-grown bacteria. The bacterial colonies showed resistance to Ampicillin; however they did not show resistance to Tetracycline. The results suggested that Tetracycline was much more effective in killing the bacterial growth, in comparison to Ampicillin. It was confirmed that a single dose of Tetracycline had killed the surrounding bacterial growth, as the bacterial colonies around where the drug had been placed, died and showed no growth. The bacterial plates also suggested that Ampicillin was ineffective in killing the bacteria, as zones of inhibition were not present, despite using two disks of this drug. These results could possibly be explained by the properties of these antibiotics and the bacteria used in the experiment. Bacterial enzymes, such as beta-lactamase, cannot be broken down by Ampicillin. The bacterial plasmid carrying this enzyme disables the Ampicillin placed in the agar, allowing for more bacterial growth. In this case, it is…show more content…
Primarily, such labs help researchers become aware of the importance of antibiotic resistance. As this is a vital issue, researchers and scientists must have a clear understanding of this concept in order to help prevent antibiotic resistance in humans. However, in order to understand how to prevent antibiotic resistance, researchers must understand the severity of this issue. In the past year, the World Health Organization released a report indicating that the world is headed into a “post-antibiotic area.” In other words, infections or injuries which have been treated effectively in the past now have the ability to put the population at risk for more diseases (Smith et al., 2002). By thoroughly studying such a major issue, it may become much easier to find possible solutions and avoiding it from becoming a hazard to the human

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