Amylase Experiment

The effects of the ketone bodies on ammoniogenesis in spite of the urinary pH and bicarbonate falling is not in any way related to why there was a partial correction of the extracellular acidosis. The metabolic acidosis occurred from production of acid within the body. Metabolic acidosis can also occur when the kidneys are not removing enough acid from the body. When metabolic acidosis occurs, this will cause the pH level to be low which is likely due to increased production of hydrogen ions and the bodies inability to form bicarbonate within the kidneys. So that is why the ion exchange of the pH had an effect when it was infused.

In the Oxidative fermentation tube the media was a differential media that helps determine whether specific bacteria can oxidize or ferment to metabolize glucose. Citrate test checks to see which bacteria could citrate as the only source of carbon. A positive test shows that an alkaline environment ia created and that the pH level rose. The color of the media changed from green to blue if its positive.

However, for trials 1 and 2, the glucose concentration barely increased, possibly due to human error. (see table #2) In those first two trials, I mixed the bean solution with the water that rests on top of it, simply because I didn’t know that it would make a difference. After those two trials, I noticed that the results weren’t changing, and only then did I decide to dispose the bean water. Disposing the bean water made a dramatic difference, since the results increased drastically afterwards.

However, after refluxing for a while, yellow precipitates begin to form near the top of the flask. It was assumed that the remaining starting material was concentrated from a decrease volume to reappeared in solution. Nevertheless, this may have been a sign of contamination that will negatively affect the entire reaction. This observation later resulted in a yellowish

Zn(s) +2H+(aq) Zn2+(aq)+H2(g) Reduction of copper Sulfate: As more zinc was added, the color of the solution changed from a light blue to a foggy white. The zinc bubbled and changed from silver to red. After the solution stopped bubbling this meant that the reaction was then complete.

Tert-butyl-chloride would be expected to never react in a SN2 reaction, as it is so unreactive under these conditions. For each of the molecules used in this experiment (except tert-butyl-chloride), the nucleophile, iodine, would attack the electrophilic carbon bonded to the leaving group, chloride or bromide. That leaving group would then take the

It was found that the compound was solid and white in color. The unknown compound was then tested solubility in water and the compound was soluble in the water. The flame test was performed for four know compound calcium chorine, sodium chlorine and ammonium chorine and the unknown compound. When unknown compound was put on the fire different color are produce. When we smell the unknown compound it indicated the presence of chorine.

This aqueous solution was then heated until all the dichloromethane evaporated off. An error could have occurred at this point in the experiment if the hot plate was too hot. If the hot plate was set above the boiling point of the ketone, the ketone could have evaporated of along with the dichloromethane. This would result in a lower percent yield of the ketone. To prevent this from happening, the hot plate should not exceed 130˚C, so no matter what ketone was isolated, it would not evaporated off.

Based on our initial observations(appearance) of solid #4 and the fact that they are not soluble in water and will sink, my partner and I have concluded that solid #4 are small pebbles. Solid #2 are small pieces of tin foil. We believe this because solid #2 isn’t soluble in water and doesn’t float just like tin foil. We also think this because of our initial observations(appearance). Nika and I believe that solid #6 is dried up purple food coloring because once you put the solid in water it dissolves and the water becomes purple(just like the color of our sludge).

Inoculate the test agar medium: There are two types of inoculation that can be done. Phenol Red Broth – Glucose, Lactose, Sucrose The test results are as follows: for glucose, lactose, and Unknown 361 tested A/-, meaning that acid was produced, but no gas and it tested K for sucrose meaning that there was alkaline production. Procedure: 1.

It is also responsible for the bactericidal activity of isoniazid. Isoniazid has inhibitory effect on mycolic acid synthesis and seems to be its main purpose. Evidence suggests that after treatment with isoniazid, there is a lack of fatty acids on mycobacterium cell wall. Also inh A enzyme acts as molecular target for isoniazid inhibition. This enzyme plays a role in prolongation of the fatty acids that contribute to mycolic acid synthesis.

Our hypothesis was that if the plate contains only the LB broth the E. coli bacteria would have no antibiotic resistance and would not glow. If the plate contains just LB broth and ampicillin then the E. coli bacteria will have antibiotic resistance only if the plasmid is present. If the plate contains LB broth, ampicillin and arabinose then the E. coli bacteria will glow fluorescent under a UV light and it will have antibiotic resistance. Similar to our expectations our results suggested that our hypothesis was correct. This is due to the fact that n order for the E coli.

The first test was Orthonitrophenylgalactophyranoside (ONPG), which tests for lactose fermentation, and my result was colorless so it was negative. Next was Arginine Dihydrolase (ADH), and my result was yellow /orange so it was negative. My results for Lysine Decarboxylase (LDC) were yellow/orange, which told me my unknown was negative.

The phenol-red indicator helps to identify the bacteria. Upon viewing my results it was determined that my colonies did not ferment mannitol. This was easily observed because they remained translucent. The incubation times for all of the inoculated tests were for 48 hours (due to lab schedule, supposed to be 24 hours) and the temperature they were incubated at was at 37C. 37C is the optimal temperature to maintain the state of the agar as well as the ideal temperature for continued microbial growth to occur. My fourth and final test conducted to help identify my bacteria was an Oxidase test.

1 Uses This treatment is designed to target fat molecules so that they naturally start to break down. It is exclusively made for the fat that is located beneath the chin. Once Kybella has been injected, the fat cells are destroyed and are unable to accumulate any more fat. 2 Testimonials and Results