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Analgesic Drugs Lab Report

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Lab 8: Chromatographic Analysis of Analgesic Drugs Written by Gurleen Bhangoo Robinjot Kaur CHEM 3301-02 Professor Jeffery Crisman 21 March 2023 Abstract: Chromatography is an analytical method that is used to separate a mixture of chemical compounds into their individual components, allowing the individual components to be thoroughly analyzed. In this experiment, thin-layer chromatography (TLC) is performed to identify an unknown drug, and then column chromatography is used to separate out the components of the drug. The given unknown will contain one of the following drugs: Advil, Anacin, Aspirin, BC Powder, Excedrin, Excedrin Tension Headache, or Tylenol, where each drug will consist of a mixture of 1-3 compounds. The presence of caffeine …show more content…

It is a method used to separate mixtures of compounds based on the way they interact with porous material called adsorbent. The solvent that moves the mixture through the adsorbent is called the eluent or mobile phase. Thin layer chromatography (TLC) is a type of chromatography to help determine the identity of compounds and the purity of a compound. Based on the polarity of the compound, column chromatography is a preparative technique used to purify compounds. In column chromatography, molecules are separated depending on how different the molecules are distributed between a mobile phase and a stationary phase. As the solvent is drawn through the capillary action, the molecules contact the paper and then separate. For each spot, the retention factor (RF), which is equal to the distance the spot traveled divided by the distance the solvent front traveled, was calculated and used to determine the identity of the unknown compound. The unknown substance will either contain Advil, Anacin, Aspirin, BC Powder, Excedrin, Excedrin Tension Headache, or Tylenol. Each drug consists of a mixture of 1-3 compounds. In order to confirm the unknown substance, a melting point apparatus would be used to determine the identification and purity of the unknown substance. Results and Discussion: Figure 1: TLC plates that were used to calculate the RF values and to determine the identity of compounds in the unknown mixture. Table 1: Data comparing the RF values for the unknown compound and known standard solutions 1:1 ethyl acetate/acetic acid 1:2 ethyl acetate/acetic acid 1:3 ethyl acetate/acetic acid 200:1 ethyl acetate /acetic acid Unknown RF 1 0.10 0.15 0.26 0.27 Unknown RF

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