The first part of this lab was to get a chromatography, spinach and a quarter. The next step was to draw a line of the chromatography and rub the spinach leaf on it with the quarter. After this, the next step was to place the chromatography paper inside the tube and allow the solvent to rise to the paper. The final step was to remove the paper and mark the spots where the colors had shown up as they would disappear soon after. By doing this lab, it was possible to see all the different accessory pigments as well as the chlorophyll. The distance a pigment traveled on the chromatography paper all depended on the affinity and solubility of the pigment. If the pigment had a high affinity and low solubility, then it would remain near the pencil …show more content…
The accessory pigments, xanthophyll and carotene in this case show up on the chromatography. The reason to as why these pigments are not as prominent as the green is due to the fact that there is an overwhelming amount of green compared to the other colors. Not only that, but the main purpose that all these pigments serve is to absorb sunlight. All the colors of sunlight that the pigments absorb are the one that is not visible to a person’s eye. This is because the plant uses all these colors of light and the ones that are not absorbed is reflected. This makes it visible to a person’s eye. All these pigments are so important to photosynthesis because they essentially starts the process. This would only be because without sunlight photosynthesis would not start and the pigments get the sunlight. In addition, a pigments function is not only to provide color for a plant, it also has other beneficial uses. Specifically, anthocyanins. Besides providing color for a plant it also seems to healthful benefits. For example, protection against liver injuries, reduction of blood pressure, improved eyesight, preventing different types of diseases, and
That mixture was then filtered through a coffee filter. Nine test tubes were prepared in order to perform this dye coupled reaction. One contained 5.0ml of the potato and pH buffer mixture, 2.0 ml of hydrogen peroxide, and 1.0 of guaiacol to serve as a blank for the spectrophotometer. Four test tubes were filled with 2.0 ml of hydrogen peroxide and 1.0 ml of guaiacol, used for measurement by the spectrophotometer, each. The last four were filled with 4.0 ml of the potato and pH buffer mixture and 1.0 ml of peroxidase.
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
The ordinary colors are from chemical pigments such as chlorophyll and melanin. The structural colors are formed by the structure of the butterflies’ wings. Iridescence plays a large part in the structural coloring of
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.
Because carbon dioxide is absorbed by the plant during photosynthesis less carbon dioxide present in the chamber is a sign that photosynthesis is working. The four lights used for this experiment range across the light spectrum on both sides in order to test a wider variety of wavelengths. All lights will be placed directly on the spinach leaf at the same distance so as not to give any spinach leaf a different light intensity, which could affect the data. This experiment will be able to show which light, ranging across the light spectrum, will allow the Spinach to perform photosynthesis more efficiently.
LABORATORY REPORT EXERCISE #5 INTRODUCTION TO THE COMPOUND LIGHT MICROSCOPE, PLANT AND ANIMAL CELLS Name_______________________________Section_____Teacher______________Date________ PRE-LAB QUESTIONS - answer the following questions using your textbook and valid internet sources. Be sure to cite your sources at the end of the prelab. You can type your answers to all questions except #1 and #9 directly into this document and then submit via Canvas. Type the answers for #1 and #9 at the end of the document. 1.
A dye is a coloured substance that has an affinity, a bond with a physical surface, to the substrate to which it is being applied. Dyes are usually soluble in water. Dyes are used to change the perceived colour of an object. Dyes consist of 2 main parts: chromohore and auxochrome. Before 1856, all dyes were obtained from natural resources.
Jaspreet Singh Professor Paratore Biology 1 November 1, 2014 Spectrophotometry Identifying Solutes and Determining Their Concentration Statement of the Exercise or of the Problem The purpose of the lab experiment was to attain the following objectives: • Learning to Operate the Spectrophotometer • Construct absorption spectra for cobalt chloride and chlorophyll. Hypothesis If greater and higher concentrations of cobalt chloride are added to each solution then greater amounts of light would be absorbed by each solution. Thus a liner relationship will result in which the absorbance of a substance would be proportional to its concentration, which will be depicted, in a linear graph.
The origins of human skin colour: The origins of human skin colour remained an enigma that was to generate a multitude of misconceptions. The true source of human pigmentation was finally revealed with the discovery of the melanocyte in the 19th century. Once the amino acid tyrosine was identified to be the key enzyme in pigment formation, attention focused on elucidating the chemical structure of melanin, an enterprise that remains incomplete.
This being that through the concentrations 40% to 10% the absorption of the solute was rather low, although some spikes on the Ethanol, although an increase in the absorption at 40% in Methanol, which could show the beginning of the required concentration for the membrane to break down and let out the pigment. This could be due to that the Ethanol and Methanol was not able to disrupt the lipid bilayer and absorb the beetroots membrane that gives it it’s pigment. This showing that the time or concentration of the Ethanol and Methanol was not long or high enough for the pigment to be absorbed into the solution making the absorbance really low as there was barely any colour change on the solute. The membrane although not reacting with alcohol present and it making the water less polar, it is evident that the membrane is soluble in water and the levels are higher as the membrane was able to be absorbed by the water, increasing the solutes absorption. Propanol on the other hand was able damage the membrane with a concentration of 20% up, which caused at least 3 times the absorption rate.
The 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.
Through the titration process, we are able to identify physical changes to the mixture such as the colour change to indicate the end point of the experiment. For example, the colour changes of phenolphthalein from colourless to pink and methyl orange from red to orange and subsequently yellow. Acids produce hydrogen ions and bases produce hydroxide ions. This causes the indicator to change colour due to the colour difference from the undissociate molecules.
The ammonia: 1-butanol (1:1) solvent was the appropriate solvent to use for the column chromatography of food dye because it exhibited the properties of a good solvent system. A total 8 colored eluents were collected. The eluents had colors of pink, dark red, dark blue, dark green, light green, yellow, orange and light yellow respectively and
When carbohydrate is utilized, acids are formed which changes the colour of the medium from green to yellow
Abstract The unknown concentration of benzoic acid used when titrated with standardized 0.1031M NaOH and the solubility was calculated at two different temperatures (20◦C and 30◦C). With the aid of the Van’t Hoff equation, the enthalpy of solution of benzoic acid at those temperatures was determined as 10.82 KJ. This compares well with the value of 10.27KJ found in the literature.