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Ap Bio Lab Report

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The purpose of this lab is to determine the relationship that exists between the number of amylase gene copies and ancestral diet. As the human civilization moved forward toward agriculture the diets of humans also changed. Depending on where the humans originated would give insight to how much of their diet was starch based. My family’s geographic origins are from China. Thus knowing that the country has a high starch based diet, we would suggest that I would have a high amylase production. From my findings we notice that the R2 value is 0.886 (shown in Figure 1) which indicates that there is a strong correlation between concentration of salivary amylase and how much light is absorbed. This suggest that due to the large amount of salivary…show more content…
It is evident that there were errors present though out gel electrophoresis in terms of timing the traveling of the DNA and the photography of the gel. As mentioned before humans have between 2 – 20 copies of the gene, and that humans have at least 2 copies of every gene one maternally and one paternally. Seeing that the entire class only had one gene copy, it is evident that something must have been done incorrectly during the use of computer tools to find the Adj. Vol (INT) on a blurry image or something went wrong with the PCR mixture. Thus it is not possible to correlate the AMY1 gene copy number and the concentration of salivary amylase based on our results alone, so in the process we are also unable to disprove or prove the results obtained by the Parry…show more content…
From figure 3 and 4, it seems to show that the level of starch rises in proportion to the concentration of salivary amylase. However, it seems that there are some data points that extend much greater above the average. This means that those points can be called into question as it seems unlikely that a person could create almost 17 ml of salivary amylase (figure 4 error bars). It is obvious that out range error and standard deviations extend greatly in the negative X direction as well as the positive X direction. When taking into account of the sources of error this data, and smaller sample size, many things contribute to increasing the large data spread of error. If the dilution was not done correctly there would be a higher salivary amylase concentration. There were not many protocols securing more variables. There was no way of securing the same quality of saliva from all participants, or fixing the variables that may increase or decrease the salivary amylase production due to nutrient intake within that day of testing. If one had a large starchy meal that morning, values of amylase concentrations may have increased due to an increase in need of digestive enzymes. These errors could be corrected and avoided by taking identical diets for several days before the
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