Artemesia Nilagirica Case Study

Abstract:

Extract from the leaves of Artemisia nilagirica are investigated for phyto chemical and anti oxidant activity. Extracts from leaves of are Artemesia nilagirica investigated for phytochemical constituent and anti oxidant activity. Leaf Extract with ethyl Alcohol revealed the presence of tannins, flavonoids, alkaloids, terpenoides, saponlns, cardiac- glycosides, in the Machipatri. In view of the presence of antioxidant properties of the plant, it is aimed to understand whether the plant can be use of in fighting the malignancy and other cancer causing agents.

Introduction:
Artemisia Nilagirica is popularly known as ‘Makkipu’ and ‘Masipattiri’ in Tamil. It is an aromatic, herbaceous perennial plant belongs to Asteraceae family, which

For this, there is a continued need for new prototypes new templates to use in the design of potential chemotherapeutic agent. Natural products are providing such templates. (Devmurari V.P.et al). The essential oil of A. nilagirica contains camphor, β-caryophyllene, D-germacrene, α-humulene, 1,8-cineole, β-eudesmol, borneol, artemisia alcohol, camphene, α-gurjunene, p-cymene, terpinen-4-ol, α-pinene, α-phellandrene, δ-carene, trans-isoelemicin, lyratol, γ-elemene, (-)-linalool, thujone, azulene, thujyl alcohol, fernenol, stigmasterol, sitosterol, amyrin and its acetate( R. Badoni .et al). Traditionally, the plant and its parts are used by the tribes for the treatment of various ailments such as poor appetite, indigestion, travel sickness, parasitic infection and cold (Darsana Udayan et al). A tall aromatic perennial shrub, often gregarious, pubescent or villous throughout; lower leaves ovate in outline deeply pinnatisect with small stipule-like lobes at the base, pubescent above, white tomentose beneath, upper most smaller, 3-fid or entire, lanceolate; panicled racemer, outer flowers female, very slender, inner disk flowers fertile, bi sexual, bracts ovate or oblong, margins scarious fruits oblong ellipsoid minute achene’s. Plant Contain sesquiterpene lactones, exiguaflavone A and B, macckianin and 2-(2,4- dihydroxyphenyl)-5,6-methylenedioxy benzofuran( Devmurari V. P.et al). The

Chemicals such as wagnes reagent,chloroform,2% H2SO4, Concentrated sulphuric Acid,10%Lead acetate, Benedict’s reagent, 0.1% ferric chloride, Fehling’s solution, dilute NaOH,2% HCL, 10% Ammonia, 10%HCL, distilled water, Ethyl Alcohol are provided by the management of the college.
Preparation of solutions: a) Fehling’s solution:- A mixture of equal volume of copper sulphate, sodium potassium tartar ate and sodium hydroxide is prepared in a beaker. b)Wagner’s Reagent:- Mixing 2gm of Iodine, 6gm of potassium iodide in 100ml of water.
Collection of sample: Healthy leaves of machipatri were taken and washed under running water to remove the dust and other external pollutants. The plant leaves were air dried for few days (normally 15 to 21days).
Grinding the sample:The dried leaves are grinded to a fine powder in a mixer and the powder is collected in clean polythene bags.
Preparation of plant extract with ethyl Alcohol: Taken 10 gms of leaf powder and added 50ml of ethyl alcohol stirred it constantly for 30 minutes and the solution was kept at room temperature for 24 hours (minimum) and then filtered. The filtered solution is again filtered with watman filter paper No.3 and then it was stored at 4 degrees centigrade (in a freezer) until