Ascorbic Acid Lab Report
PRODUCTION OF ASCORBIC ACID
Several reactions are involve in production of ascorbic acid .It is from D-sorbitol to 2- Ketogulonic acid, bacterial fermentation reaction with the addition of Acetobacter suboxydans and Ketogulonicigenium vulgare act as catalyst.During the fermentation phase, there two phases present at the chemical reaction conditions which are liquid and gas.
Bacterial fermentation is an anaerobic redox process, in which oxidation of the substrate is coupled to the reduction of another substrate, or an intermediate derived from the oxidation, with the differences in redox potential and the end product providing energy for the ATP synthesis.
Before D-sorbitol is converted to 2-Ketogulonic acid , hydrogenation takes place between
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Keep the feed balanced enough to keep the growth of the microorganisms (Acetobacter suboxydans) at a desired specific growth rate and reducing simultaneously the production of by-products (that can be growth or product production inhibitory and make the system not as effective).Higher growth rate and faster oxidation can be easily achieved. A 48 hours old growth from agar slants was transferred into 10 ml medium in test tubes having the composition of D-sorbitol, yeast extract, ammonium dihydrogen phosphate, magnesium sulphate with pH 6.0. While, Acetobacter suboxydans is a neutrophiles bacteria.
Equation at batch reactor:
2- Ketogulonic acid +Water+ Acetone L- ascorbic acid + Acetone+ Oxygen + Hydrogen
C6H10O7 + H2O+ (CH3)2CO C6H8O6 + (CH3)2CO + O2 + 2 H2
A + B + C D + E + F + G
Fed-batch fermentation is one of the most available processes for obtaining maximum specific growth rate during cultivation, leading to faster oxidation of substrate into
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