Germination And Contamination: A Case Study

Culture medium

Effect of PPM, SHC and Chitosan on germination and contamination of axillary buds

The effect of PPM (0.1, 0.2 and 0.5%), SHC (0.1, 0.2 and 0.5%), CSN I (0.005, 0.01 and 0.02 %) and CSN II (0.005, 0.01, 0.02%) on germination and contamination was studied by incorporating respective concentration in PSRM. The pH was adjusted to 5.7±0.1 before autoclave and all cultures were incubated at 28±1°C with a 16 h photoperiod and observation recorded on 21st day.

Statistical Analysis

The data was analyzed by use of SPSS (software package version 16) and Microsoft Excel 2010. One way ANOVA was applied to test mean differences of all treatments while statistical significant difference between mean values was established at p ≤5% while Duncan’s

There was no superior difference in germination but contamination was significantly controlled with PPM as compared to the control. PPM (0.5%) had highest germination (96.67±3.33%) and least contamination (13.33±0.96%3.33) (Fig 2 A). An inverse relationship between contamination and germination was found when PPM concentration is increased up to 0.1 to 0.5%. Rihan et al. (2012) was reported same kind of results. This is in concurrence with Compton and Koch (2001) who found that the percentage of melon cotyledon explants which formed callus and somatic embryos and furthermore the number of embryos per explant were decreased when incubated in embryo initiation and embryo development media containing more than 5 mL L−1 PPM. Also Niedz (1998) mentioned that PPM does not affect the viability of sweet orange (C. sinensis L.) explants and does not reduce their growth ability when it is used in concentrations lower than 1.0 ml/l. Kraj and Dolnicki (2003) indicated that there was no negative effect of PPM upon the growth and development of callus in European beech. It seems that the determination of the optimal PPM concentration for controlling the contamination without affecting the growth capacity in the culture medium depends primarily on the plant species. PPM seems to have a role not just in the control of contamination in the culture substrate but also on the development and growth of artificial seeds since Rihan et al. (2011). positive effects of using PPM were also reported by Lata et al. (2009) who indicated that the addition of PPM had a positive result on overall seedling growth and a remarkable improvement in the re-growth and conversion rate of Cannabis sativa L. artificial