Aim This experiment separates the constituents of a commercial headache powder employing the use of extraction method. The active ingredients of the commercial headache powder were extracted by dissolving the powdered tablet in propanone solvent. The suspended fine particle binders in the propanone solution were removed by filtration method. The solid analgesia was obtained by evaporating propanone solvent. It was then collected on Hirsh funnel by filtration.
Equimolar solutions of both the drug and NBD-Cl reagent (1×10-3 M) and (1×10-2 M) were prepared for method I and method II respectively. Then portions of mixture of master solutions of the drug and reagent were mixed comprising different complementary proportions. Either the relative fluorescence intensity or the absorbance were measured. A blank experiment was carried out simultaneously. The stoichiometry between the drug and NBD-Cl reagent by both methods as shown in Figure 8, it is clear that the ratio is 1:1.
Reddy et al., (2011) developed the floating microspheres of Cyclobenzaprine HCl by emulsion solvent diffusion technique using Ethyl cellulose polymer to achieve an extended retention in upper GIT and there by improved bioavailability. Streubel et al., (2011) developed floating microparticles composed of polypropylene foam, Eudragit S, ethyl cellulose (EC), and polymethyl metha acrylate (PMMA) and were prepared by solvent evaporation
Therefore, liquid-liquid and acid-base extraction techniques were successfully performed to separate the components of the Excedrin tablet. According to the TLC analysis results, the compounds (aspirin, acetaminophen, and caffeine) were successfully isolated from the analgesic (Excedrin tablet). In figure 1, the separation of the compound in the TLC analysis correlates with the TLC analysis in figure 2. Furthermore, Rf index calculations of the TLC analysis demonstrated that the compounds (aspirin, acetaminophen, and caffeine) were separated. The Rf calculations of aspirin in table 1 shows an Rf value of .491; however, in table 2 the Rf value of aspirin was calculated to be .784.
A. INTRODUCTION DESCRIPTION Metronidazole is 1-(2-hydroxyethyl)-2-methyl-5-nitroimidazole B.P. It appears as a white to brownish cream crystalline substance with melting point 159-162C. Solubility in water at 20C is 1g/100mL; in ethyl alcohol, 0.5g/100mL; in chloroform, 0.4g/100mL; slightly soluble in ether and soluble in dilute acids. When reconstituted as Metronidazole IV for Infusion, it has a pH of between 4.8 and 5.2.
The antioxidant activity of the extracts was measured on the basis of the scavenging activity of the stable DPPH free radical (as cited in Dong et al., 2014). Evaluation of antioxidant activity of astaxanthin through DPPH assay was modified according to the procedures reported by Lewis (2012). 12 mls of 0.1 mM DPPH solution with methanol was prepared. A measurement of 0.005 g of DPPH was added to 12 mls of methanol which was measured with a graduated cylinder into a small foil-wrapped flask. A number of 11 two ml microcentrifuge tubes were assembled and was labeled as: Tubes 1a-c through 3a-c: Product Extract Dilution 1 through 3 (repeat three times for 9 tubes), Tube 4: Positive control, α-tocopherol and Tube 5: Negative control, solvent only.
Complexation techniques(1,13,12) Different methods can be used to transport out the complexation of the cyclodextrin with drug. approximately of which are described lower: 1.4.1. Physical blending/milling/co-grinding: Mechanical energy is used to mixture, mill or co-grind the drug and the cyclodextrin. 1.4.2. Kneading Technique: In this technique Cyclodextrin is initially taken in mortar then drug is gradually further into it and using small percentage of water it is triturated using pestle to get a paste like uniformity.
Hanusaiodine solution, chloroform, aqueous KI solution, Na2S2O3 and starch solution is used. Iodine values are calculated from the difference between the blankaand the test sample. For peroxide value; solvent mixture (composed of glacial acetic acid and chloroform), saturated KI solution, starch solution and Na2S2O3 soluiton is used and peroxideavalues are calculated. A) Iodine Value: Hanus Method In this experiment, iodine value of sun floweraoil was determined with Hanusamethod. Blank solution and oil solution were prepared and stored in the dark.
Morpho-physiological and Biochemical Responces of Rubus idaeus under Salinity Stress In vitro Conditions Supplemented by Sodium Nitroprusside Ali Ghadakchi asl , Ali- Akbar Mozafari*1, Nasser Ghaderi1 Abstract. To evaluate the effect of sodium nitroprusside (SNP) on morpho-physiological and biochemical characteristics of Rubus idaeus var. Danehdrosht, shoot tip explant was cultured on Murashige and Skoog (MS) medium supplemented with growth regulator combination including Benzyleadenin (BA)(1 mg.L-1), Indol-3-Butyric Acetic acid (IBA)(0.2 mg.L-1), Sodium Nitroprusside (SNP) (0, 50 and 100 µM) and NaCl (0, 50 and 100 mM).The results showed that salinity stress significantly decreased morpho-physiological and biochemical characteristics such as RWC, MSI, total soluble protein in regenerated explants. Furthermore salinity significantly increased the total
pruriens seed extract and FeMPn were characterized with FTIR to determine the biomolecules contained in the extract that involved in the reaction to form FeMPn. The FTIR spectra of the extract and FeMPn are shown in Fig. 5. The FTIR spectrum of the extract showed a broad absorption band in an absorbance area of 3384.8 cm-1 that assigned to the overlapping of O-H stretching vibration of flavonoids, alkaloids, polyphenols, alcohols or water and N-H stretching vibration of amine compounds, due to the hydrogen bonding. The absorption band at 1627.8 cm-1 referred to C=C stretching vibration which is possible to be derived from aromatic ring in amino acid, while the absorption band in 1529.4 cm-1 referred to N-H bending vibration of amine which is possible to be derived from the L-dopa.