B Galactosidase Lab Report
This experiment involved the chosen enzyme, B-Galactosidase, to be tested with a substrate called o-nitrophenol-B-D-galactopyranoside (ONPG). The purpose was to determine over time the effects the enzyme had on the substrate concentration, as well as to examine the effect of lactose, a disaccharide on the formation of o-nitrophenol. The experiment utilized a spectrophotometer to determine at which the rate that the enzyme catalyzes, by timing the change in absorbance every 15 seconds, as well as observing any colour change. The amount of enzyme added to the B-Galactosidase is increased over time, and the ONPG is set to a constant value each trial. It was determined that through the trials of testing the absorbance of the enzyme, the faster
Alpha Galactosidase Lab Report
1488 Words | 6 PagesBackground: Many people experience excessive gas, bloating, and discomfort in the intestinal areas. While there are other contributing causes, food is the one variable that mainly causes these problems, such as: broccoli, beans, dairy products, grains, etc. Beans, for example, contain oligosaccharides that makes it hard for the body to digest. These carbohydrate rich foods makes digesting difficult because they all contain high-fiber, starch, lactose, etc. Beano® is a product that helps aid in digestion.
Beta Galactosidase Lab Report
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Turnip Peroxidase Lab Report
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Enzyme Fast Lab
485 Words | 2 Pagespurpose the propose of this experiment was too see if the chemical reaction of a enzyme can be made faster. Hypothesis I think that a warm environment would be best to make an enzyme’s reaction faster. because a protein can move faster in heat.
Lab Report On Enzyme Activity
1419 Words | 6 PagesSucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1.
Laboratory Report on Enzyme Activity
1407 Words | 6 PagesLABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
Enzyme Lab Report
1778 Words | 8 PagesIn this lab, the optimal environment for an enzyme was observed in bacterial and fungal amylase. An enzyme is a substance produced by a living organism that is coded by proteins to catalyze chemical reactions throughout the body. Enzymes are what make the chemical reactions in living organisms possible. Enzymes act as catalysts to reactions, they lower the activation energy needed for reactions. However, enzymes require particular environments to function, and without proper conditions, enzymes
B-Galactosidase Lab Report
1764 Words | 8 PagesEnzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. Enzymes are fragile and easily disrupted by heat or other mild treatment. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside (ONPG) used in place of lactose. Upon hydrolysis by B-galactosidase, a yellow colored compound o-Nitrophenol (ONP) is formed.
The Biological Temperature Of Catalase
750 Words | 3 PagesIntroduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).
Bio Chem: Lab Report Of Amylase In Enzyme
916 Words | 4 PagesBio Chem lab Report 04 Enzyme Biochemistry Group Member: Chan Man Jeun Duncan (16002621) Law Sze Man (16000478) Introduction Enzyme is a protein base structure substance in our body. It works at a biocatalyst that will catalyzing the chemical reaction, which helps to speed up the chemical reaction. Enzyme could only function in specific shape, and the shape of enzyme is depending on the environment, therefore it is hard for an enzyme to function well in an extreme environment. The aim of this experiment is to see can the enzyme functions normally in different environment(pH, temperature and salt concentration) via using starch solution, amylase from saliva, 0.5M HCl solution, 0.5M NaOH solution and NaCl solution, and using iodine solution
B Amylase Lab Report
513 Words | 3 PagesThe difference in diastatic malt products and non diastatic malt products is the difference in ezyme activity. Diastatic malt products have a considerable amount of enzymatic activity. The action of B amylase on undamaged/ungelatinised starch is extremely slow. A amylase attacks starch very quick.
Michaelis Menten Model Lab Report
943 Words | 4 PagesBy observing figure 3, the more enzyme that is available, the faster the reaction rate is. The optimal enzyme concentration was chosen based on the R2 values from figure 2. The highest observable rate also had the best R2 number, which was closest to one. This enzyme concentration was used in part 2.
Effect Of Enzyme Concentration On Enzyme Reaction
1046 Words | 5 PagesABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.
Arginase Lab Report
1437 Words | 6 PagesINTRODUCTION: Arginase is an enzyme- enzymes are biological catalyst which drives a reaction at the speed of life. Arginase is a hydrolase, hydrolases catalyze hydrolysis reactions, this is determined via the E.C number (Nelson and Cox 2008). Arginase has the EC number is 3.5.3.1 (Schomburg 2015). The enzyme ‘commission number’ is the arithmetical classification that is used for enzymes which indicates the chemical reaction they catalyze.
Glycation Lab Report
802 Words | 4 PagesADVANCED GLYCATION END PRODUCTS Advanced glycation end product as an oxidative process that occurred in food perserved or heated the presence of reducing sugars were first observed in early 1900s which non-enzymatically make the food proteins insoluble with yellowish fluorescent products attached to them. This process is known as Maillard reaction named on its discoverer the Louis C. Maillard in 1912 (Finot ., 2005). The Maillard reaction is the one of physiological significance. In vivo, it is also observed that human protein non-enzymatically glycated in the presence of physiologic sugars. One of the best characterized glycation product known to occur in the body is glysocylated hemoglobin.
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