This study was conducted with a partner, since some parts of the experiment were able to be done simultaneously. One partner prepared a TLC developing jar by pouring a small layer of 4:1:1 propanol/acetic acid/water into a developing jar. A solvent wick was made by wetting a piece of filter with the solvent, and it was placed in the jar. A silica coated TLC plate was obtained, and a spotting line was carefully drawn approximately 1.5 cm from the bottom of the plate using a pencil. Extra care was taken to not touch the plate with bare skin.
3.12.2 Oxidase Test On a filter paper or cotton bud, pick the desired colonies from the agar plate. Then, using a dropper, take the oxidase reagent and drop it on to the filter paper and observe for colour changes. The blue colour appearance indicates positive reaction whereas if there are no changes, then it’s a negative reaction. 3.12.3 Genus Verification using TCBS agar The TCBS medium, known as Thiosulfate Citrate Bile Salts Sucrose Agar is a recommended selective medium which allows the growth of bacteria belonging to the genera Vibrio. TCBS agar was prepared for about 100ml and poured into petri plates, after solidifying, pure colonies of bioluminescent bacteria was streaked.
Mix both the ingredients and massage it like a scrub all over your face. Then, leave it for 5 to 10 minutes and wash it off. Chickpea: A chickpea flour pack helps in exfoliating your facial skin by removing dead skin as well as unwanted hair. The added benefit is that it also lightens your skin tone along with lightening facial hair. 2 tablespoons of chickpea flour, 1 tablespoon of milk or cream, 1 teaspoon of turmeric.
Before beginning the four phonemic awareness assessments, I sat down with Rheya and discussed the assessment with her. I explained to her that we were first going to review her knowledge of capital letters of the alphabet, lower case letters of the alphabet, letter sound identification, and then we were going to work on isolating initial phonemes. I explained the four skills in terms that she would understand. As an incentive for her cooperation, I brought a small bag of fruit snacks and told her that she would get to eat the fruit snacks after school if she participated during the four skill
Using the micropipette, we transferred the appropriate volume of algae to the jars. We collected a sample of brine shrimp and added them to petri dishes in order to view them underneath a dissection microscope. Because we were testing the top-down effects, we decided on having
It is often used in the selective identification of enteric bacteria including Salmonella and Shigella. The TSI agar has glucose, lactose and sucrose as the sources of carbohydrates. Phenol red is the acid base indicator incorporated in the medium. The TSI medium indicates whether the bacteria ferments glucose only, or lactose and sucrose with or without production of gas. Nitrate serves as a source of nitrogen for many bacteria.
To clean the syringe, flush it by drawing 6 mL of distilled water. Step 2: Mix both test tubes , shake gently and time the reaction. Step 3: The same step as procedure 1, and step 3 which is to record the observed color step 4: use the palette/color chart to help you identify the observations you make. Safety precautions: Pull your hair back Safety eye goggles Closed toe
Use these home remedies to remove tanning from your darkened neck: • Rice Powder, Milk and Honey – Mix two tablespoons of milk with two teaspoon rice powder and one teaspoon of honey. Prepare a fine paste and apply it on your neck. Massage gently on your neck for 20 minutes. This paste acts as a scrub and alleviates all the dead skin cells and removes tanning from your skin. • Lemon Juice and Cucumber – Just mix two teaspoon each of lemon juice and cucumber juice and apply this paste on your neck and wash off after 10 minutes.
3.10.2Column Packing Before starting to pack a column, a small piece of cotton is gently will insert into the centre hole of column with the aid of a long stick. A small amount of n-hexane will add into the column. Silica gel weighted is mixed with n-hexane to form a mobile slurry solution and then slowly transferred into column. The side of the column will gently tape to prevent the formation of air bubbles in the column. Additional solvent will use to add the remaining silica gel into the column.