Antibacterial Manufacturer: Melastoma Bacteria

TABLE OF CONTENT

PAGE

1.0 INTRODUCTION

1.1 Background of study 1

1.2 Problem statement 3

1.3 Significance of study 3

1.4 Objective of study 4

2.0 LITERATURE REVIEW

2.1 Melastoma malabathricum Linn. (Senduduk) 5

2.2 Phytochemical Screening 7

2.3 Test Organisms

2.3.1 Bacteria 8

2.3.2 Antibiotic 8

2.4 Modified Kriby-Bauer Disc Diffusion Method 8

2.5 Minimal Inhibitory Concentration (MIC) 8

2.6 Minimal Bactericidal Concentration (MBC) 9

3.0 METHODOLOGY

3.1 Material

3.1.1 Raw Material

3.1.2 Test Organisms

3.1.3 Control Antibiotic

3.1.4 Chemicals

3.1.5 Apparatus

3.2 Method

3.2.1 Collection and Preparation of sample

3.2.2 Sample Extraction

3.2.3 Phytochemical Screening

3.2.4 Antibacterial

For E. coli bacteria, will be cultured on MacConkey agar, while for S. typhimurium, will be culture on (Mueller Hinton Agar) MHA. The purpose of culturing the bacterial strain on specific agar is to obtain the pure culture of the bacteria. All plate will be incubated at 37oC for 24 hours to allow the growth of bacteria (Cavalieri et al., 2005).

3.2.4.3 Preparation and Standardizing Inoculums Suspension

4-5 colonies of each bacteria strain will be taken by using inoculating loop and suspended in 8 ml of 0.85% saline solution that adjusted to 0.5 McFarland standards in test tube separately and mix well. Then, all test tubes will be incubated for 24 hours at 37oC to get active strains (Cavalieri et al., 2005). Table 3.2.4.3 shows the preparation of 0.5 McFarland Standard Turbidity and 0.85% saline solution

Table 3.2.4.3 Preparation of standard and saline solutions.