ipl-logo

Bacterial Amylase Reaction Lab Report

206 Words1 Page

In this experiment students were able to see enzyme activity and learn about how the bacterial amylase and the fungi amylase react at different temperatures when mixed with starch. We were able to see when the enzyme is catalyzing using iodine. As stated in the Results, the only temperature tested which showed a lighter coloration was 55 degrees. The lighter coloration is due to enzyme activity, which means that starch was being catalyzed by amylase and turned into maltose.
Maltose gives the light coloration in the mixtures, which is the reason why at lower temperatures the colors of the spots were dark brown, and as time passed by they became slightly lighter. The more maltose present in the mixture, the lighter the spot will appear. At

Show More
Related

  • Bio 101 Lab Report

    602 Words  | 3 Pages

    Name: Avishak Deb Roy Partners: Leevell Penn, Varugh, Butler Bio 101 Lab Report #1 02.22.2018 Swimming speed of paramecium tetraurelia in different levels of treatment. Introduction Paramecia is a unicellular Protista which are naturally found in aquatic habitats. It is easily cultured in the laboratory. It is oblong shaped and covered with short hairy structure called cilia. Paramecia does not pose any health or ethical concerns and the population can be maintained if there is a food source such as Enterobacter (Biological Foundation 7).

    Read More

  • Escherichia Colo Lab Report

    308 Words  | 2 Pages

    Transformation in bacteria usually takes place when a bacterial cell accepts strange DNA and integrates to its own DNA. The transformation normally takes place within plasmids, which are tiny circular DNA molecules that have been separate from its own chromosome. The copies of the same plasmid range from 10 to 200 copies within a cell. These copies of plasmids may multiply when the chromosome replicate or multiply independently. One plasmid has a range of 1,000 to 200,000 base pairs.

    Read More

  • Enzyme Lab

    901 Words  | 4 Pages

    Introduction: Enzymes are needed for survival in any living system and they control cellular reactions. Enzymes speed up chemical reactions by lowering the energy needed for molecules to begin reacting with each other. They do this by forming an enzyme-substrate complex that reduces energy that is required for a specific reaction to occur. Enzymes determine their functions by their shape and structure. Enzymes are made of amino acids, it 's made of anywhere from a hundred to a million amino acids, each they are bonded to other chemical bonds.

    Read More

  • Catalase Lab Report

    251 Words  | 2 Pages

    Hypothesis: Very high temperature will denature and decrease the rate of enzyme reaction. Very low temperature will also decrease the rate of enzyme reaction. Procedure: In this experiment, 3 pieces of potatoes were put into 3 different test tubes of varying temperatures.

    Read More

  • Nitrogen Fixing Bacteria Lab Report

    1448 Words  | 6 Pages

    Effect of different physical conditions on nitrogen fixing bacteria from rhizosphere Hypothesis: Rhizospheric Nitrogen fixing bacteria show optimal growth at PH: 6-7, Temperature: 30 °C and Salinity level: 0.005 – 0.010M NaCl INTRODUCTION: There is a huge bacterial diversity in rhizospheric soil. Gram-negative, non-sporulating baccilli which respond to root exudates are predominant in the rhizosphere (Pseudomonas, Agrobacterium). While Gram-positive bacilli, Cocci and aerobic spore forming bacteria like Bacillus and Clostridium are rare in the rhizosphere.

    Read More

  • Lab Report On Enzyme Activity

    1419 Words  | 6 Pages

    LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.

    Read More

  • Macromolecules Lab Report

    1486 Words  | 6 Pages

    1% glucose, 1% maltose and 1% lactose all progressively get positive results by changing colours to reddish brown at the end of this experiment. In this case the aldehyde functional group that is present in the products (monosaccharides and some disaccharides) in this reaction is able to reduce copper in the presence of alkali and this produces colour changes while converting to an aldose sugar. Honey is made of fructose and glucose which instantly turned brown after the test-tube was placed in the boiling water because of its active aldehyde and carbonyl group. The copper (II) sulphate present in the Benedict’s solution reacts with electrons from the aldehyde group which results in a redox reaction to from cuprous oxide, a red brown precipitate that seen in all of the above mentioned solutions (Hill, 1982). Beer also gave positive results because it contains aldehydes and ketones (i.e. acetone, trans-2-butenal, furfual) during its beer production process where the sugars are converted through fermentation (Hill, 1982).

    Read More

  • Laboratory Report on Enzyme Activity

    1407 Words  | 6 Pages

    55 degrees celcius Table 6: Effect of Sucrose Concentration on Sucrase Activity Optical Density 35 g/L 30 g/L 25 g/L 20 g/L 15 g/L 10 g/L 5 g/L 0 g/L 1 1.007 0.974 0.950 0.926 0.849 0.734 0.515 0.003 2 1.002 1.011 0.947 0.937 0.834 0.766 0.496 0.002 3 0.980 0.998 0.944 0.932 0.838 0.754 0.495 0.001 average 0.996 0.994 0.947 0.932 0.840 0.751 0.502 0.002 Effect of Sucrose Concentration on Sucrase Activity 5. State how sucrase activity changes with increasing sucrose concentration. First sucrase activity increases greatly. After 10 g/l sucrase activity continues to increase but at a slow rate until it reaches 30 g/l. At 30 g/l to 35 g/l sucrase activities mostly stayed the same

    Read More

  • An Internal Investigation Of The Digestion Of Starch By Salivary Amylase

    848 Words  | 4 Pages

    Logically, the expectation is that the higher the concentration of saliva, the more breakdown of starch will occur and a brighter shade of blue will be produced because less starch will be detected by the iodine

    Read More

  • B-Galactosidase Lab Report

    1764 Words  | 8 Pages

    B-galactosidase breaks down the disaccharide lactose into simple sugars glucose and galactose. However, glucose is a colorless compound hence it has to be substituted with a compound that is detectable by a visible color change. Hence,

    Read More

  • Amylase Enzyme Lab Report

    833 Words  | 4 Pages

    Enzymes. Lipids (Lipase) Lipase are secreted by the small intestine and pancreas. They digest complex lipids (fats) molecules into simpler, more soluble fatty acid and glycerol molecules. They exist of fats and oils and are made up of the elements carbon, hydrogen and oxygen and the most common type known of is the triglyceride.

    Read More

  • Effect Of Temperature On Amylase

    1167 Words  | 5 Pages

    Uncontrolled Environmental conditions Atmospheric conditions The controlled variable Concentration of amylase was kept under control by measuring the amount of amylase used and also it was made sure the percentage of amylase used was 1%. The Amount of amylase/starch used were kept to 5cm3 at all times. Materials needed Beakers Bunsen burner Test tube Thermometer Stopwatch Test plate Glass rod Starch Amylase solution Water bath Iodine solution. Test tube holder Labels Marker Procedure First 5 test tubes were taken and labeled with numbers from 1 to

    Read More

  • How Temperature Affects Bacterial And Fungal Amylase

    523 Words  | 3 Pages

    How Temperature Affects Bacterial and Fungal Amylase Activity Vanessa Romero Vanessa Romero 0058506 Group 1: Vanessa Romero, Kayla Montero, Aixa Andion-Arias, Sophia Tavarez Biology 1010L Section U36 Abstract: Several experiments were conducted on bacterial and fungal amylase, examining the rate at which both break down starch at various temperatures. Enzymes act as catalysts to accelerate reactions. Amylase is a type of enzyme that catalyses the breakdown of starch into sugars. In order to record the rate at which starch was broken down at different temperatures, bacterial amylase was placed in water baths of 0°C, 25°C, 55°C, and 85° for five minutes.

    Read More

  • What Happens When Can Yeast Metabolize?

    451 Words  | 2 Pages

    These results accept the hypothesis: if yeast can metabolize, then the bromothymol blue solution should turn yellow from the production of carbon dioxide. Only the bromothymol blue solution with yeast turned yellow, suggesting that the yeast caused the color change. The yeast consumed sugar, produced

    Read More

  • Lab Report On Fermentation

    1432 Words  | 6 Pages

    Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.

    Read More

More about Bacterial Amylase Reaction Lab Report

Open Document