Bacterium growth on various agar plates Introduction The purpose of this experiment is to show different agar plates inhibit or enable growth of different organisms. Some varieties of media enable the grow of a wide range of organisms such as nutrient agar. Other media are selective which means they contain specific nutrients to encourage the growth of certain organisms. This means other organisms will die due to the selective nutrients such as high concentration of salt which will cause plasmolysis. Differential organisms require different nutrients which show it is fairly easy to isolate and identify organisms by using selective media.
You use cUTI plates as the chromogenic media and find that some of the colonies have turned pink in colour. What information does this tell you about the bacteria in the sample, and why did the colony change colour? [2 marks] This tells me that the bacteria in the sample contains coliforms such as Escherichia coli (E.coli). The colonies changed colour as the enzymes produced by the bacterial cells reacted with the red galactosidase in the agar medium, this reaction causes the colonies to turn pink making them easily
Traditionally, it has been always prepared with sugar and black tea. The sugar and black tea is added to a pan and boiled for several minutes. Tea infusion is cooled to about 30oC. Later, pour tea into the glass container with a wide mouth and add the Kombucha culture. Close the mouth of the bottles with the cloth and rubber band to prevent contamination of insects, bacteria, mold and dust and for a constant supply of air.
The effects are red bright skin. In lab, we learned to distinguish two types of bacteria using the gram stain, gram positive and gram negative. Since S. Aureus has a thick peptidoglycan cell wall with teichoic acid, it stains gram positive. Further tests will indicate the bacteria, including the presence of catalase, sheep blood hemolysis, mannitol fermentation, halotolerance, and coagulase, which S. Aureus is positive for all. 3.)
Our results from the PCR process were very unexpected, even to the point the control colony had some rather odd outcomes. The goal of this experiment was to choose three colonies from the petri dish that has been exposed to +Amp, and look for any signs of the +Amp resistant gene, blaTEM, within the colonies and decide if this gene does have an impact on bacterial resistance towards the antibiotic. My partner and I decided to utilize a bacterial colony sample that does have blaTEM genes as our control group for us to indicate what a blaTEM gel strand would appear in the agarose gel results. When observing the product of the gel product after gel electrophoresis, we were surprised to find out none of our three colonies had any strands that indicated the presence of blaTEM despite each of them surviving through the exposure to this antibiotic.
Introduction Our world is composed of many bacteria’s’ that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods that have been learned in the laboratory to determine our unknown bacteria. Bacteria’s can have different features, shapes, and or arrangements that help microbiologist determined their role in our life (whether they are good or bad for humans). Bacteria can be classified as gram positive or negative (difference in call wall).
Figure 2. Column chromatography set-up After setting up the column, 2 10-ml of the chosen solvent was obtained and was placed in two separate test tubes. Using a dropper, ~0.5 mL of the food dye was put into the column by dropping it at the side of the column in a circular motion. The chosen solvent was then added just after the green food
Extension of the technique includes expunging the desired “band” from a stained gel viewed with a UV transilluminator. • In order to visualize nucleic acid molecules in agarose gels, ethidium bromide or SYBR Green are commonly used dyes. Illumination of the agarose gels with 300-nm UV light is subsequently used for visualizing the stained nucleic
to adhere and colonize human intestinal mucosa/ epithelial cells is a major criterion for selection as probiotic or commensal bacteria. Adhesion to the host tissues is a prerequisite first step of bacterial colonization which is generally mediated by cell surface adhesion proteins . Adhesion of probiotic bacteria not only help in persistence of bacteria in gut but also participate in pathogen exclusion by competition and blocking of their binding sites at the mucosa . This adherence to epithelial cells on mucosal surface is modulated by specific binding of microbial adhesins. Lactobacilli adhesins can be broadly classified according to their targets in the intestinal mucosa (i.e.
You may include flowcharts and sketches, if it is helpful. (15 pts) There are three parts of the immune system that consist of the body’s natural barriers, the innate immune response and the adaptive immune response. The skin and mucosal surfaces form barriers against infection. When bacteria enters through a break in the natural barrier of the skin, it is first introduced to antimicrobial peptides under the surface of the skin that is supposed to kill bacteria,
GFP was needed so that we would tell if the ampicillin resistance gene had been properly placed when the bacteria glowed under a UV light. The purpose of this lab was to perform a procedure known as genetic transformation which allowed us to genetically engineer E. Coli to be ampicillin resistance. Before the lab we expected that lysogeny broth and minus DNA will have growth but no glow. The lysogeny broth, ampicillin, and
Tn 4351 was originally isolated from bacteroides fragilis  . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation . Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides.
The enzyme urease tends to trigger inflammation. Once those chemicals have been converted, the acidity in the mucous surrounding the bacteria then becomes neutral protecting the bacteria. Even with the immune system being strong, the bacterium does not get abolished due to Helicobacter Pylori creating components in the cell wall that are similar to molecules made in
Good and bad bacteria exist in our environment, depending on which one enters the body the immune system these bacteria can either compromise or aid the immune system’s illness fighting capabilities. In class we learned that the skin aids in fighting infection by acting as a protective barrier. In addition, we discussed how normal flora is found on the skin and aids in the defense mechanism because it fights off potential threats by competing with them. The normal flora consists of microorganisms such as protozoa, fungi and bacteria. The normal flora will compete with the foreign bacteria for nutrients and space and has the ability to push out or starve the invader as said in class.
All of the plates were looked at for the amount of colonies grown, if growth was present, and if the colonies gained the ability to glow green. Transformation was successful in the plates where the bacteria consumed the pGLO plasmid. In the first plate that the bacterium was plated on it included the LB broth and of ampicillin antibiotic (amp), 2 colonies were present. The second plate of bacteria was grown with the presence of LB broth, ampicillin, arabinose sugar (ara), and 22 colonies were observed. But a green fluorescent glow of the colonies was only present in plate 2.