Results: Table 1: Results from the Salicylic acid (g) and the Purity of ASA (g) Experiment Mass of salicylic acid (g) 0.14 g Mass of filter paper (g) 0.47 g Mass of “impure” ASA + filter paper (g) 0.56 g Mass of prepared “impure” ASA (g)
Next, is the verification and determination of pure liquids. A clean and dry a 25mL graduated cylinder must be gathered from the lab cart, weigh the dry cylinder to the nearest mg and record the data. Add distilled water to the cylinder making sure the water level is at above the 20mL mark but below the 25mL mark. Determine and record the temperature of the water in the cylinder. Then, reweigh the cylinder to the nearest milligram.
0.5 mL of AuNPs solution was added to the above mixture. The UV-Vis spectra were recorded with a time interval of 1 min in a scanning range of 200-600nm at ambient temperature (25±20C). Antimicrobial activity The agar disc diffusion method was employed for the determination of antimicrobial activity of the papaya leaf extract stabilized gold nanoparticles. The 0.1 ml of 108cfu/ml of different pathogenic bacteria suspension was spread on different plates nourished with LB media. Filter paper
In addition, phenolphthalein was added as an indicator. The aliquots were titrated against sodium hydroxide (NaOH) solution until end point was reached, after which volume of NaOH consumed was recorded. The value of the rate constant, k, obtained was 0.0002 s-1. The experiment was then repeated with 40/60 V/V isopropanol/water mixture and a larger value of k = 0.0007 s-1 was obtained. We concluded that the rate of hydrolysis of (CH3)3CCl is directly proportional to water content in the solvent mixture.
Results 1 mole of benzoic acid (C6H5COOH = 122.12grams) reacts with 1 mole of methanol (CH3OH = 32grams/mole) to produce 1 mole of methyl benzoate (C6H5COOCH3 = 136.15grams) and 1 mole of water. 5.0grams of benzoic acid Molar mass of benzoic acid: 122.12g/ml 5.0g122.12g=0.041moles 19.75grams of
The antioxidant activity of the extracts was measured on the basis of the scavenging activity of the stable DPPH free radical (as cited in Dong et al., 2014). Evaluation of antioxidant activity of astaxanthin through DPPH assay was modified according to the procedures reported by Lewis (2012). 12 mls of 0.1 mM DPPH solution with methanol was prepared. A measurement of 0.005 g of DPPH was added to 12 mls of methanol which was measured with a graduated cylinder into a small foil-wrapped flask. A number of 11 two ml microcentrifuge tubes were assembled and was labeled as: Tubes 1a-c through 3a-c: Product Extract Dilution 1 through 3 (repeat three times for 9 tubes), Tube 4: Positive control, α-tocopherol and Tube 5: Negative control, solvent only.
Table 12: interpretation of FTIR spectra of naproxen Bonds Standard (/cm) Observed (/cm) O-H stretch 3600-3500 3421.41 C=o stretch 1750 1740.39 C-O stretch 1100 1076.62 C=C stretch 1650-1550 1569.68 Comments for compatibility: All IR spectra of pure compound and its composition with excipients in formulation are same. This indicates that there was no structural change caused by excipients. 7.2 Optimization of Process Variable The preparation procure was accordingly optimized and validated on the basis of following process variable Table 13: Effect of temperature Temperature Cholesterol (mg) Tween 80 (mg) Tween 20 (mg) Drug (mg) % Entrapment 40?C -60?C 50 100 100 50 Not Formed Above 80?C 50 100 100 50 Color change Table 14: Effect the concentration of cholesterol and drug Sr. no. Cholesterol (mg) Tween 80 (mg) Tween 20 (mg) Drug (mg) % Entrapment 1 50 100 100 100
The three petroleum ether extracts were combined and concentrate to its minimum volume by using a stream of nitrogen. Analysis of fatty acids methyl ester (FAMEs) was carried out by Gas Chromatography using( Hewlett Packard, Palo Alto, CA, USA) (HP 6890) and(FID) detector was used at 250 °C . The fatty acid methyl siloxane capillary column HP – 5 (30m x 0.32 mm I.D. × 0.25 μm film thickness) was used. Nitrogen was used as the carrier gas (0.8 m / min gas flow).
Record results to determine if HCl increases or decreases the pH of the water. 14. If the HCl in the solution increases the pH of the water, it shows that HCl will increase the pH of the blood and if the HCl in the solution decreases the pH of the water, it shows that HCl will decrease the pH of the blood. 15. Add 10ml of 0.85% lactic acid into a beaker containing distilled water.
Design: Typically, the column design comprised placement valves for top-to-bottom sampling, and 14 thermocouple sensors. The concentration of methanol in solution was calibrated from theoretical refractivity as MeOH = -20.525 × 1.32888 + 28.226 (mol. /L). The power supplied was varied to determine the efficiency of spring-batch distillation at different power settings. In this setup, it was assumed that ChemCad sizing estimations are accurate and that the column would operate 24 hours/day for 366days.
The dried roots of Inula racemosa were pulverized and sieved with 100 ~ 200 mesh. The herb powder was placed into a glass bottle. Ultrasound-assisted extraction was carried out in an ultrasonic cleaner RK102H (Bandelin sonorex, Germany). The powder of Inula racemosa was extracted three times under the following conditions: the ratio of material to solvent was 10:1, undergoing ultrasonic treatment 30 minutes at 25 °C, 100 kHz /450 W.31 Before large extraction, a small-scale extraction experiments were carried out: 95% ethanol and ethyl acetate as the extractive solutions was investigated, respectively. The extraction efficiency was evaluated according to the percent content of AL and IS contained in the dried roots of Inula racemosa and calculated