Beetroot Comparative Study

COMPARATIVE STUDY OF BIOACTIVE COMPOUNDS OF BEETROOT AND PINEAPPLE FOR THE TREATMENT OF PSORIASIS AND EVALUATION BY PSORIATIC KERATINOCYTE CELL LINES

SANJANA S. SHETTY1 AND MAHESH KUMAR*2

1M.Tech scholar, Food and Nutritional Biotechnology, SRM University,

2Assistant Professor, (SG), Department of food and Process Engineering, SRM University, Tamil Nadu, India

*Corresponding Author Email [e-mail] [e-mail] Telephone [phone]

ABSTRACT: The present study investigates the comparative study of ethanolic extract and freeze-dried powder of Beetroot (Beta vulgaris) and Pineapple (Ananas comosus). Betalain obtained from

Beetroot juice is a powerful tool to prevent infectious and malignant disease. It is also a potential source of valuable water-soluble nitrogenous pigments, called betalains.4 The intense red color of beetroots is derived from high concentrations of betalains. Betalains are used as natural colorants by the food industry. Betalains are especially important for phytomedicine.5 It has also been studied for possible health benefits in humans, especially their antioxidant and anti-inflammatory activities.6 About 0.5-1% of extracted beet juice solids are betalains. Betalain pigments have specifically been shown to possess various antioxidant functions. Beetroot paste has been used for application on ulcers, inflammation and skin troubles Beets also contain small amounts of other nutrients like Vitamin A, Vitamin C, calcium, copper, magnesium, iron etc.7 The bioavailability of betalains in humans is reported to be high, and they remain stable in the gastrointestinal tract without any significant loss in anti-oxidative and anti-inflammatory properties, which increases their value as health food

The turbidity of the cooled samples was measured at 660nm. The experiment was performed in triplicate.20 The Percentage inhibition of protein denaturation was calculated as follows. Percentage inhibition = (Abs Control –Abs Sample) X 100/ Abs control

(v) Determination of Anti - Psoriatic Activity

• MTT assay Chemicals and reagents: MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) invitrogen, USA. Acridine orange were obtained from Sigma, USA. All other fine chemicals were obtained from Sigma–Aldrich, St. Louis.

• Cell Culture

HaCaTcells obtained from NCCS (National Centre For Cell Science, Pune) were cultured in Dulbecco 's Modified Eagle Medium (DMEM), supplemented with 10% fetal bovine serum, penicillin/streptomycin (250 U/mL), gentamycin (100ug/mL) andamphotericin B (1mg/mL) were obtained from Sigma Chemicals, MO, USA. All cell cultures were maintained at 37◦C in a humidified atmosphere of 5% CO2. Cells were allowed to grow to confluence over 24 h before