36) The left and right beaker are emptied and clean to begin the next trial. 37) The 200 (MWCO) Dialysis Membrane was placed between the left and the right beaker. 38) Nine millimolar of Na⁺Cl⁻ is placed inside to fill the left beaker. 39) Deionized Water is placed inside to fill the right beaker. 40) A sixty-minute timer was started to see how the 9 Na⁺Cl⁻ (mM) solvent diffuses through the 200 (MWCO) Dialysis Membrane.
After the 30 minutes is done, the tests tubes are then immersed in a 100 degrees Celsius methanol water bath for 15 minutes. Once the samples become frozen, put in a lyophilizer at a temperature of -109 degrees Celsius. The samples are allowed to completely dry. After 6 hours remove from the lyophilizer. Acetonitrile at a PH of 7 (neutral) is added to each of the test tube samples.
DMF was used as a solvent and AIBN (0.5% w/w of total monomer) as free radical initiator .The reaction was carried out at 70±2° C for 6 hour with constant stirring. After completion of the process it was cooled to room temperature and resultant polymer solution was poured in the large amount of methanol with stirring when polymer precipitated out. It was filtered and washed with methanol. The polymer was purified by repeated precipitation using methanol from solution in DMF and then it dried. 2.3 Preparation of PS
A control extract is prepared (5ml of DAE) to a test tube, which is then placed in boiling waterbath for 10minutes, after 10minutes remove the control extract and leave it to cool at room temperature. In order to determine the amylase activity, one drop of iodine is dropped into 21 labelled wells on the ceramic test plates. A reaction mixture is prepared, 5ml of buffer and 1ml of 0.5% starch solution to a test tube. Extract one drop from the reaction mixture to the well labelled T. Turning blue-black indicating the presence of starch in the reaction mixture. Add 1 ml of diluted amylase extract to the reaction mixture.
In a typical procedure, TiO2 nanoparticles (TNP, 3.6 g) with pure anatase was added into a NaOH (10 N, 150 mL) solution in a teflon lined stainless steel autoclave, sonicated (2 min) and heated the autoclave at 403 K for 48 h in an oil bath under autogenesis pressure with stirring (250 rpm). After 48 h, the autoclave was cooled down to room temperature, subsequently the formed nanotube was washed with ultrapure water until the pH of the solution was >7. Afterward the nanotubes were washed with HCl (0.1 M) solution for overnight under stirring at room temperature. Then the nanotubes were filtered (Millipore filtration assembly) under vacuum pump and repeatedly washed with ultrapure water until the filtrate was free from chloride ion, which was checked by the addition of silver nitrate to the filtrate. Finally, the obtained nanotubes were dried in an oven at 343 K for 12 h and calcined in a muffle furnace at 773 K for 4 h. The synthesized nanotube was abbreviated as
This diluted solution will be used in the assay as duplicate samples. Then, 1.0mL of standard glycine solution containing (7.5mg/mL) was diluted to 100mL with water using a volumetric flask. This solution contains 1.0µmole/mL of glycine. 8 tubes were set up according to the following protocol and 2.0mL of ninhydrin reagent was then added to each of the 8 tubes and were placed in a boiling water bath for 20 minutes. After 20 minutes, the tubes from the bath was carefully removed, cooled in a beaker of cold water, then 8.0mL of 50% ethanol was added and mixed well.
Materials Urea, Oxalic acid, Y(NO3)3‧6H2O, Cu(NO3)2‧3H2O, Ba(NO3)2, 95% C2H5OH 3. Procedure a. Weigh 40 g urea and 3.15 g of oxalic acid, put them into a 250 ml Erlenmeyer flask. Add 18 mL water. Heat them at 70 ˚C until the mixture totally dissolve.
We then slowly added 25ml of chilled deionised water to the filtrate to initiate crystallization by using a measuring cylinder and a dropping pipette, once we had done this we left it for about 10 minutes to allow crystallization at room temperature. We then weighed a filter paper which we will use later in the experiment. We then collected the crystallized acetylsalicylic acid by vacuum filtration in a Buchner funnel and washed the product with a little ice-cold water. We then pre-weighed a clean, empty watch glass and labelled it with our initials and the date, we did this do we could easily identify that it was ours when we go to weigh it with the crystals on. We
2. Experimental 2.1. Catalyst preparation The CuMnOx catalyst was prepared by the co-precipitation method, the aqueous solution manganese acetate (Mn(CH3COO)2.4H2O) and copper (II) nitrate (Cu(NO3)2.2.5H2O) were premixed by stirring for 1 hour. After the proper mixing of the copper nitrate and manganese acetate solution, it was added to the aqueous KMnO4 solution by a burette under the stirring conditions. After dropped completely the copper manganese solution into the precipitant ageing for 2h, then filtered, washing several times with hot deionized water.
In a separate beaker, 10-3 M of synthesized SB was dissolved in 10 ml DMF. The two solutions were mixed together under stirring and resulting yellow precipitate solution was transferred to a sonochemical bath. After 60 minutes of sonochemical treatment, the resulting CdS precipitate was collected, filtered, washed with double distilled water and absolute ethanol several times to remove the unreacted chemicals, and finally dried in an oven at 80oC for 5hours. Similar procedure was adopted to synthesize uncapped CdS
The silver ion TLC was prepared through the following procedure: Silver nitrate was dissolved in 10 ml of distilled water. This aqueous solution of silver nitrate was absolutely mixed with 9 g of silica gel (10 ~ 40 μm particles). Then, a 10 × 5 cm TLC plate was coated with the above slurry and activated for 1 h at 90 °C before use. They were immediately transferred into a desiccator in dark for storage after cooling. 32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate.
To a stirred solution of acetanilide (2gm, 0.5moles) in dry acetonitrile (5ml) at 0-5⁰C, TiCl4 (2.8gm, 1.0moles) was added drop wise with stirring and the mixture was stirred at room temperature for 30 min. Then Sodium azide (0.93gm, 0.5 mol.) was added to it and the reaction mixture was heated at 80-90⁰C. After 2 hrs remaining amount of sodium azide (0.93gm, 0.5 mol.) was added to reaction mixture and heating was continued for 4-6 hrs.