This reaction is refluxed in a waterbath for two hours and their volume were reduced to 70% of it’s original volume and residue was obtained. The coloured product obtained was filtered under suction, washed with ethanol. The product were recrystallized from ethanol. Their yields ranges from 50-55%, the product obtained were pinkish colour and melting point was 2020C. Step-III: Synthesis of Cr(II) Complexes: The Schiff's base complexes were synthesized by mixing the Schiff's base (1.5 g) in ethanolic solution of Chromium chloride [CrCl2].
Stored in light glass bottle. 2.5.2: Formation of PANI(CoFe2O4) 0.1g of Nano material and 50ml of Aniline hydrochloride were mixed in a beaker. Then 50ml of Ammonium peroxydisulphate was added drop wise in reaction mixture with constant stirring below 20 oC. After 24 hours the coated sample was filtered, washed and dried at 60 oC in oven and then grinded into a fine powder in agate
The peel powder was soaked overnight (or for desired period) at room temperature (30-32°C) for extraction with intermittent shaking. After extraction, it was centrifuged at 2000 rpm for 5 min and filtered through Whatman No. 2 filter paper. The effective volume obtained after centrifugation was noted. 2.3 Determination of radical scavenging activity (RSA) Determination of RSA was carried out according to Murthy, Jayaprakasha, & Singh (2002) using DPPH as stable free radical and butylatedhydroxyanisole (BHA) as standard.
The system was heated for 4-5 hr under vacuum at 200°C and then cooled down to the temperature (50°C) where we want to perform the adsorption study. Small doses of test gases consecutively introduced to the system and gradually increased up to 50 Torr until an equilibrium pressure was reached. Then the obtained differential heats of the test gases adsorption were recorded as a function of its coverage. Further, the manifold degassed under vacuum for almost 30 minutes; adsorption was conducted in the same manner. Finally, the number and strength of active surface are obtained from the difference between the adsorbed gases from the first and second
A 50 mL buret was obtained and was washed with NaOH solution. After filling the buret with NaOH (titrant) and preparing the KHP (analyte) in the Erlenmeyer flask, the solutions were titrated. The volume used from the NaOH solution was recorded. C. Determination of the Acidity of Soft Drinks First, the soft drinks were heated. Upon cooling, it was shaken until no bubbles were formed.
Density functional theory (DFT) based ab initio calculations were done to monitor the formaldehyde (CHOH) adsorptive behavior on pristine and Pd -decorated graphene sheet. Structural optimization indicates that the formaldehyde molecule is physisorbed on the pristine sheet via partly weak van der Waals attraction having the adsorption energy of about -15.7 kcal/mol. Metal decorated sheet is able to interact with the CHOH molecule, so that single Ni atoms prefer to bind strongly at the bridge site of graphene and each metal atom bound on sheet may adsorb up to four CHOH. The findings also show that the Ni decoration on graphene surface results in some changes in electronic properties of the sheet and its Eg is remained unchanged after adsorption
Apparatus- Chromatography column: C18 (10 microns particle size), with Guard column Flow rate: 1.2ml/min Pressure: 30-40kgf Wavelength: 326nm Mobile phase: methanol : water (95:5 v/v) Internal standard: retinyl acetate Injection volume: 20µl Procedure for Retinol extraction from serum samples- 1) 100 µl of serum sample and 100 µl of Retinyl acetate were added into 12 X 100mm glass test tubes. Vortex-mixed for 30 seconds. Then, kept them at 4 C for 5 mins. 2) 1mL of hexane was added and vortex-mixed intermittently for 60 sec. 3) Centrifuged at 2500 rpm for 12 mins.
Add 75 Ml Conc.HNO3 and heat the flask on a boiling water bath. On heating the flask sugar dissolves in nitric acid and copious evolution of nitrous fumes comes out. Discontinue heating. The reaction subsides in about 20 minutes. Pour the hot solution into a porcelain dish, wash the flask with 15 mL of nitric acid and evaporate the acid solution on a water bath
4: (a) Crystal structure of rho-ZMOF (left) and schematic presentation of [H2TMPyP]4+ porphyrin ring enclosed in rho-ZMOF R-cage (right, drawn to scale). (b) Cyclohexane catalytic oxidation using Mn-RTMPyP as a catalyst at 65 ◦C.  A similar method was used for the heterogenation of a cationic homogenous catalysts. Developed by Genna et al. the technique involves the cationic exchange of guest molecules in the framework of the MOF.
After 20 minutes, the tubes from the bath was carefully removed, cooled in a beaker of cold water, then 8.0mL of 50% ethanol was added and mixed well. The absorbance of each tube was determined at 570nm and the absorbance were plotted against amount of standard glycine. For determination of tyrosine content using Folin & Ciocalteu’s reagent, seven tubes were set up. Then, 1.6mL of 0.5M Na2CO3 was added to each of the tubes and were mixed thoroughly. 0.4mL of the Folin & Ciocalteu’s reagent then were pipette in, mixed thoroughly, and allowed to stand for 10 minutes.