For example, grow CMT 167 cell line in RPMI 1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin solution. Warm medium to 37 °C in hot water bath prior to use. 5. Prepare 1x cell culture medium separately as you would for normal cell culture of the cell line of interest. 6.
persica Binomial name Salvadora persicL.Salvadora persica (Arak, Galenia asiatica, Miswak, Peelu, Pīlu, Salvadora persica, or toothbrush tree, mustard tree, mustard bramble), is a types of Salvadora.  Salvadora persica has antimicrobial properties.  Used for a considerable length of time as a characteristic toothbrush, its sinewy branches have been advanced by the World Health Organization for oral cleanliness utilize. Investigate recommends that it contains various medicinally valuable properties including abrasives, sterilizers, astringent, cleansers, chemical inhibitors, and fluoride. Distribution]Native to: Algeria, Angola, Botswana, Cameroon, Chad, Egypt, Eritrea, Ethiopia, India, Iran, Israel, Jordan, Kenya, Libya, Malawi, Mali, Mauritania, Mozambique, Niger, Nigeria, Oman, Pakistan, Palestine, Saudi Arabia, Senegal, Somalia, South Africa, Sri Lanka, Sudan, Syria, Tanzania, Uganda, Yemen, Zambia, Zimbabwe Also happens in Namibia.
Quinazolinone is a building block for approximately 120 naturally occurring alkaloids isolated till date from a number of families of the plant kingdom, microorganisms and animals. The first quinazolinone, i.e., 2-cyanoquinazolinone was synthesized in the late1860’s from anthranilic acid and was found to possess anti-convulsant activity. Interest in the medicinal chemistry of quinazolinone derivatives was stimulated in the early 1950’s with the elucidation of quinazolinone alkaloid i.e, 3-[ keto-(3-hydroxy-2-piperdiyl)-propyl]-4-quinazolone from an Asian plant Dichroa febrifugin, which is an ingredient of a traditional Chinese herbal remedy, effective against malaria. Methaqualone was synthesized for the first time in 1951 and it is the most well- known synthetic quinazolinone drug, famous for its sedative and hypnotic
However, it is more commonly viewed as the primary metabolite and active compound of acetyl salicylic acid, which has been used as an anti-inflammatory drug by physicians for over 100 years. Recently, it has been discovered that prophylactic use of acetyl salicylic acid can reduce the risk of cardiovascular disease and certain cancers. (Sammuganathan et al,
Medium and culture conditions Cells were grown in a 100-mL flask containing 25 mL into minimal medium supplemented with Eugenol for 2, 4 and 6 days incubation at 37 °C. The following Table 3. represents the composition of the modified minimal media used for biotransformation of eugenol (Muheim and Lerch, 1999). The pH of the medium was adjusted between 7.0 to 7.25 and autoclaved to obtain sterilized media for further
Materials All the chemicals used for synthesis purposes were of AR grade, purchased from Sigma Aldrich chemicals, and for spectral analysis spectral grade solvents were used. Double distilled water was used for preparing solutions. The test strains, Escherichia coli (E. coli) gram-negative, Staphylococcus aureus (S. aureus) gram-positive bacteria and Pseudomonas aeruginosa (P. aeruginosa) were purchased from IMTECH, Chandigarh, India. Yeast extract, tryptophan and bacterial-grade agar–agar were purchased from Hi-media Laboratories, Mumbai, India. Synthesis of 2-[(4-methoxy-phenyl)iminomethyl]-4-nitrophenol (SB) The Schiff-base, 2-[(4-methoxy-phenyl)iminomethyl]-4-nitrophenol, (C14H12N2O4) was synthesized by using p-Anisidine in methanol
There are thousand species of medicinal plants used globally for the cure of different infections. These plants are used as antimicrobial agents and several works has been carried out by scientists to find out its scientific basis (Omotayo, 1998) which include Anacardium occidentale, Pilostigma recticulatum, Anogeissus leiocarpa, Enantia chlorantha, Senna occidentalis and Azadiracha indica. The use of medicinal plants predates the introduction of antibiotics and other modern drugs in the African continent. Herbal medicine has been shown to be effective and over 60% of the Nigerian population depends on traditional medicine for their health care needs (Ghani et al., 1989). Traditional medicine practitioners in Nigeria use a variety of herbal preparations to treat different kinds of ailments including typhoid fever, paratyphoid fever, dysentery, malaria, diarrhoea and wound infections.
[b]History of Herbalism[/b] [b]Overall history[/b] It has been stated by archeologists that we have had a relationship with herbs going back 30,000- 50,000 years. Although in ancient times herbalism, much like the way of life, was generally mixed with superstitions and magic. In the 17th century medicine and botany went separate ways. Today we can determine superstition from that of scientific fact. Many of the traditional herbs have been tested as well as proven to be beneficial to health.
Case study Name Professor Institution Course Date Executive summary Lorex pharmaceutical, deals with the production of chemicals that are used in curing diseases in human beings. The company has identified another drug that is effective in curing hypertension referred to as Linatol. The drug was produced by the company and was patented under Lorex many years ago. The company found Linatol an effective drug that could solve the problems associated with high blood pressure. The dug was then accepted by the Food and Drug Association and approved for use in curing the disease (Bodily, Carraway, Frey & Pfeifer, 1998).
They were the pioneers in using plants as valuable source of medicine. There are numerous Sanskrit writings which explain the usage, collection and isolation of drugs from plants. The Assyrians, Babylonians and ancient Hebrews were all familiar with the use of plants as drugs. Some of Egyptian papyri, written as early as 1600 B.C., mention plants such as Cannabis, Opium and Cassia being used by the physicians. The Greeks were familiar with the works of Aristotle, Hippocrates, Pythagoras, and Theophrastus.