The membrane permeability of Beta vulgaris is affected by ethanol solution Introduction Cell membranes are the semi-permeable membrane that surrounds all cells. It separates the extracellular environment from the intercellular environment. It is a phospholipid bilayer which contains various proteins, lipids and carbohydrates all serving different purposes. It is this structure which allows for the transport of nutrients, proteins and water. (Nature.com, 2014). Through extensive testing it has been found that small alcohols, specifically ethanol can increase the fluidity and membrane permeability of the phospholipid bilayer (Patra et al, 2005). The aim of the experiment was to test what effect that ethanol solution would have on the membrane …show more content…
Vulgaris cell. For this experiment it is hypothesised that exposure to ethanol solution will increase the membrane permeability of the B. Vulgaris cell. Methods The experimental methods were taken from Flinders University (2018). The aim of the experiment was to test what effects that ethanol solution has on the membrane permeability of B. Vulgaris. The B. Vulgaris samples were approximately 1cm3. They were kept the same size to ensure accurate results. A control test was conducted in distilled water to obtain a result to compare. The ethanol treatments were 40% and 70%. To prepare the solutions a 70% ethanol solution was used to make 40%. This was calculated using the C1V1=C2V2 formula. A photo spectrometer was used to measure, in arbitrary units, the change in membrane permeability of the B. Vulgaris cells. To begin, the B. Vulgaris samples were put into vials containing the distilled water, 40% and 70% Ethanol …show more content…
More specifically the aim was to investigate what effect 40% and 70% ethanol solutions had on a B. Vulgaris cell membrane and then compare them to the same test with distilled water. It was hypothesised that the ethanol solution would increase the membrane permeability. From the results the hypothesis can be supported. Cell membranes are a core aspect of understanding cells which helps to understand humans and other living creatures. Therefore the topic of cell membranes has been extensively researched, meaning that there is no limit to information and sources of information of the subject. The effects of alcohols on membrane have also been researched quite extensively. Previous studies have found that alcohols disrupt the structure of cell membranes (Goldstein, 1986). They have also found that ethanol has a stronger effect than other alcohols (Patra et al, 2005). From this research and literature the expected results of the experiment were to see an increase in membrane permeability, this result was observed. The study provides a brief insight into what kind of effects alcohol can have on cell membranes but it is limited. The results can be used to understand more practical applications, like human cells for example. the experiment is ineffective way of observing the effect of alcohol on cell membranes and is a good education tool. The study is
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A Demonstration of Chemotaxis Between Flies and Various Substances (Sugar vs Bacteria) Abstract: The purpose of this lab based on the Drosophila melanogaster (the common fruit fly) reactions. Since the fly has been studied and observed for many years, and known for its unique chemotactic attractions to different stimuli, it was an ideal organism for the study being conducted. In the experiment the purpose was to be able to figure out whether the flies would be more attracted to sugar or bacteria.
Methanol, has a molecule containing CH3OH it being the smallest, ethanol having more carbons and hydrogens than Methanol comes second, CH3CH2OH, and propanol, CH3CH2CH2OH, is the largest of the three molecules. Aim: The aim of this experiment is to see the effects of different alcohols and concentrations on the biological membrane of the beetroot. Research Question: How do different alcohols and concentrations affect the biological membrane of a beetroot? Hypothesis: As the alcohols increase in toxicity and concentration, the damaging and breaking down of the membrane will increase leading to more red pigment being released into the solution causing the absorption of the solute to increase.
Lysosomes digest the toxins so they can be safely eliminated by the cell. Peroxisomes contain oxidases and catalases that detoxify substances like alcohol and neutralize free radicals. The smooth endoplasmic reticulum (ER) also contains enzymes that detoxify certain drugs (such as alcohol). 3. Mrs. Fender’s jaundice is caused by the accumulation of bilirubin in her blood and tissues.
Alcohol metabolism in the liver will produce a large number of free radicals, excessive free radicals scavenging ability than the body, will react with the cell membrane, causing lipid peroxidation, resulting in damage to liver cells, liver cells and enzymes are released into the blood, related serum biochemical index increased. From the results of this experiment, compared with the blank group, the levels of ALT and AST in the liver of the model group were increased, and the difference was statistically significant (P<0.01), which showed that the alcoholic liver model was successful. The administration group could promote the increase of GSH-PX and SOD activity in the liver of mice, and inhibit the increase of MDA content in liver tissue, and then protect the protective effect of acute alcoholic liver injury in
It is known that alcohol can cause damage and functional problems to protein, carbohydrates, and fat metabolism (mezzo). Due to this knowledge, it was expected that E2F transcription factor proteins would be affected by alcohol. In order to perform this experiment, HT-1080 cells were acquired from a fibrosarcoma of a 35-year-old human male. The concentration of protein in the HT-1080 cells was determined and then Western analysis was performed. Image J software was used to analyze the Western Blot in order to determine protein density and determine if there was a significant change of E2F isoforms density when exposed to
Alcohol’s effect on the fruit flies was very evident because the fruit flies started to lose their strength to fly around the bottle. As the concentration of ethanol increases, the flies tend to get uncoordinated and oblivious with their surroundings. In other words, the fruit flies tend to get tipsy, and once the alcohol concentration increases, the fruit flies become sedated. It was also observed that too much exposure in ethanol could lead to the fruit flies’ death. This means that fruit flies are very weak when it comes to tolerating the presence of alcohol and they could reflect withdrawal symptoms (Ledford,
Diffusion and Osmosis Lab Report By: Jettica Williams BIOL 1107 Lab September 21, 2016 Prepared for Mrs. Fulford Lab Course Page Break The cell membrane act as a roadblock for cells. The cell membrane has a very hectic job. It restricts the access to what comes in and what goes out. The bond the membrane shares with others is the idea of accountability.
Part A: Osmosis practical task Aim: To observe the effects of osmosis in rhubarb cells. Hypothesis: Water will be extracted out of the cells in the salt solution causing the cells to look different to the cells in the freshwater solution. Materials: Rhubarb Distilled water in a dropping bottle Salt solution in a dropping bottle Microscope, slides, and coverslips Forceps and razor blades or scalpel Paper Towel Method: Clean and dry a slide and coverslip.
DETERMINATION OF PERCENTAGE ETHANOL IN BEVERAGES 1. Introduction to Gas Chromatography Gas chromatography is a very powerful separation technique for compounds that are reasonably volatile. The components of a sample partitions into two phases, the 1st of these phases is a immobile bed with a great surface area, and the other is a gas phase that permeates through the immobile bed. The sample is evaporated and passed by the mobile gas phase or the carrier gas through the column. Samples separates into the stationary liquid phase, based on their solubilities at the given temperature.
What is the effect of temperatures 10°C , 20°C, 40°C, 60°C and 70°C ± 1/°C on yeast fermentation when baking bread? ii. Aim: The focal aim of this experiment is to investigate the effect that temperature has on the growth and respiration of yeast (Saccharomyces cerevisiae) fermentation. iii.
This experiment is to investigate the relationship between solute concentration and the movement of water through semipermeable membrane by the process of osmosis. The purpose of this The Visking tubing apparatus establishes the osmosis procedure. The Visking tubing is a semipermeable membrane filled up with concentrated sucrose solution. The surface of the semipermeable membrane symbolizes the visking tubes and the mixture demonstrates the cytoplasm. If the Visking tube is absorbed in water, after a period of time, it will be have water inside water, this is because the water molecules can pass through the tubing, while the larger sugar molecules cannot diffuse out from the tubing because the size of sugar molecules do not allow it to go through the tubing.
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.