Betamethase Research Paper

2.1 DRUG PROFILE

2.1.1 Betamethasone Valerate

INTRODUCTION[4][30][31][32][33][34]
Name

Betamethasone Valerate
Official in Indian Pharmacopiea, British Pharmacopiea, United state Pharmacopiea
Approval Status CDSCO - 2001
Description

A glucocorticoid given orally, parenterally, by local injection, by inhalation or applied topically in the management of various disorders in which corticosteroids are indicated. Its lack of mineralo corticoid properties makes betamethasone particularly suitable for treating cerebral edema and congenital adrenal hyperplasia.

Structure
CAS no 2152­44­5
Solubility
Freely soluble in chloroform; soluble in ethanol (95 per cent); practically insoluble in water and in light petroleum.

“Simultaneous determination of Neomycin sulphate and Polymyxin B sulphate by Capillary Electrophoresis with Indirect UV detection” [50]
Stationary Phase: fused silica capillary with 50 μm i.d. and 27 cm total length at an applied voltage of 6 kV
Mobile Phase: 15 mM phosphate run buffer (pH 5.0) containing 40 mM N-(4-hydroxy-phenyl)acetamide and 50 mM tetradecylammonium bromide (TTAB)
Detection: 280nm

7. “Flow Injection Chemiluminescence determination Of Neomycin in Pharmaceutical Formulations” [51]
Procedure: The chemiluminescence reaction is based on the enhancing effect of neomycin on the chemiluminescence reaction between luminol-hydrogen peroxide catalysed by potassium ferrocyanide in the Triton X-100 alkaline media. Neomycin solution was injected into a water carrier stream and then mixed with hydrogen peroxide in the presence of Triton X-100 solutions. The reaction mixture was then merged at a Y-piece with a reagent stream consisting of potassium ferrocyanide and luminol in alkaline solution.
Detection: The elicited chemiluminescence intensity was measured by a photomultiplier tube operated at a voltage of 880 V.
Range: 0.1–14 µg mL-1

8. “TLC for Neomycin Sulphate” [52]
Stationary Phase: silica gel
Mobile Phase: 3%ammonium:Acetone