This organism was cultured under solid-state fermentation for 72 h using wheat bran as the substrate. After 72 h, crude enzyme was extracted from the culture medium. The fibrinolytic enzyme was purified from the crude sample by various steps: ammonium sulfate precipitation, dialysis, ion exchange chromatography, and casein-agarose affinity chromatography. All purification steps were performed at 4°C until otherwise stated. The crude enzyme was precipitated at 70% saturation of ammonium sulfate, and the protein was collected by centrifugation (10,000×g for 10 min).
(2, 4, 14-18) Alkaline pH (NaOH) Creatinine + picrate red chromophore (absorbs at 510nm) EQUIPMENTS & APPARATUS: Siemens Dimension® clinical chemistry analyzer (X pand and RxL Max) Flex reagent cartridge, Assay Cups, tips, pipettes. Flex – CREA REAGENT REQUIRMENTS: Reagent
Neomycin sulphate was a gift of INTAS Pharmaceuticals Ltd. (Ahmedabad, India). Silver nitrate, Sodium borohydride, Polyvinyl Pyrrolidone K-30 (PVP K-30), Chitosan, Surgical Gauze and Silver sulfadiazine ointment were purchased from Qualigens fine, Merck, S. D. Fine Chemicals, Balaji Chemicals, Medicare Hygiene and Super formulation Pvt. Ltd, Ujjain respectively. All other chemicals and solvents were of Analytical Reagent grade. Milli Q water was used throughout the experimental work.
ABSTRACT NRC-04, a novel antimicrobial peptide derived from skin mucous secretions of flat fish winter flounder, shows a broad spectrum of antimicrobial activity. In order to understand the conformational change of NRC-04 in different types of membrane, our team did experiments on NRC-04 with negatively charged bacterial surface membrane mimetic micelles sodium dodecyl sulphate(SDS), zwitterionic eukaryotic middle membrane mimetic micelles dodecylphosphocholine(DPC), gram-negative bacteria outer membrane mimetic micelles Lipopolysaccharide(LPS) and bacterial inner membrane mimetic micelles 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol(POPG). Fluorescence test shows that the C-terminus tryptophan residue of NRC-04 interacts with the hydrophobic
Magnetic susceptibility and photoluminescence are analyzed after the calcination process using magnetic susceptibility balance (Sherwood Scientific, Appendix Figure 27) and fluorescence spectrometer with excitation wavelengths range from 370 nm to 600 nm, emission wavelength 390, excitation slits set at 5.0 nm and the scan speed of 500 nm/min (PerkinElmer fluorescene spectrometer LS 55, Appendix Figure 28). In the calcination process, the furnace was activated in the morning of the day (around 10a.m) and was off in the evening (at 5p.m). The synthesis of strontium ferrite is using the same pathway by using only strontium nitrate and iron(III) nitrate-9-hydrates. In sol-gel process, this process is a method to establish a sol and allow formation of gel and removal of solvent. The metal or metalloid element is surrounded by different kinds of ligands and they are suspended in the medium called precursors.
 Next to no clinical information is at present accessible to bolster the utilization of fosfomycin for nonUTI diseases. For fosfomycin disodium, average every day measurements in patients with typical renal capacity go from 12 to 16 g directed 6-, 8-or 12-hourly i.v. as a bolus or 30 – hour long infusion.  Nonetheless, day by day dosages as low as 1 g and as high as 24 g have been reported, As have longer mixture times (of 4 h89). Fluctuation in the measurements controlled is in all probability because of the absence of data with respect to proper PK/PD focuses for maximal bacterial impact.
(Chiu and Ou 1996). The PCR reaction mix contained 6.25 μL Promega GoTaq Green, 0.5 μL invA-F and invA-R primers, and 4.5 μL distilled water, and 0.75 μL DNA template. DNA amplification was done in a 12.5 μL reaction volume under the following conditions: initial denaturation at 95 oC for 2 min, 30 cycles of denaturation at 95 oC for 30 seconds (s), annealing at 60 oC for 30 s and, extension at 72 oC for 30 s, with a final extension at 72 oC for 5 min. Salmonella enterica serotype Typhimurium provided by the Natural Sciences Research Institute (NSRI) of the University of the Philippines Diliman and nuclease-free distilled water were used as template for positive and negative control,
Title of Invention "AN IMPROVED PROCESS FOR THE PREPARATION OF CAROTENOIDS FROM ENCYSTED HAEMATOCOCCUS CELLS" Abstract An improved process for the preparation of carotenoids from Heamatococcus cells characterized in simple method of extraction of said caratonoids by using acidified aqueous solution, which comprises suspending encysted Heamatococcus cells in acidified aqueous solution having concentration in the range of 0.01 N to N at a temperature range of 8-90° C for a period of 30 seconds to 15 minutes, separating the encysted cells by conventional centrifugation and getting the desired carotenoid preferably astaxanthin by solvent extraction of said acidified aqueous solution obtained after centrifugation. Full Text The present invention
“SYNTHESIS AND CHARACTERIZATION OF NICKEL OXIDE BY USING UV/VIS SPECTROMETRY, SCANNING ELECTRON MICRSCOPY, FOURIER TRANSFORM INFRARED SPECTROSCOPY AND ITS ANTIMICROBIAL ACTIVITY AGAINST BACILLUS SPP., PSEUDOMONAS SPP. ESCHERICHIA COLI” J.C. Pradeep Kumar, Dr. F. V. Dandawate Assistant Professor, Department of Chemistry, Dr. D. Y. Patil Arts, Commerce & Science College, Pimpri, Pune – 411018. (Maharashtra, India) ABSTRACT Nanobiotechnology combines biological principles with physical and chemical procedures to generate nano-sized particles with specific functions. A green synthesis of nickel oxide and nickel synthesized by boiling method using an extract of Molinga Olefera. UV/Vis-Spectra showed the maximum absorbance of 280 nm in the
To determine the antimicrobial and the biofilm inhibition of hydrogel films at 100:0, 95:5, 85:15, 75:25 and 50:50 solutions in Staphylococcus aureus, Candida albicans and Pseudomnas aeruginosa Questions What solvent may be used to extract Hyptis suaveolens (L.) Poit? How can the phytochemicals present in Hyptis suaveolens (L.) Poit be determined? How can the Nanochitosan powder be extracted from shrimp shells and be characterized in terms of molecular weight and crystalline structure? What will be the measurements of the physical and mechanical properties of the produced hydrogel films from Nanochitosan/Hyptis suaveolens (L.) Poit and Alginate/Hyptis suaveolens (L.) Poit in terms of in terms of thickness, transparency, tensile strength, swelling behavior and gel fraction? What will be the effects of the hydrogel films on the growth and biofilm formations of the Staphylococcus aureus, Candida albicans and Pseudomnas aeruginosa?