Biochemical Test Essay

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IDENTIFICATION OF UNKNOWN BACTERIA USING BIOCHEMICAL ANALYSIS
Introduction:
Bacteria play an important role in human life; they play a major part in decomposition of organic material, they are present in our digestive tract, some even fix atmospheric nitrogen for the plants. But at the same time there are many species of bacteria which can cause harm to living organisms. Identification of these bacteria plays a vital role in medicine and biological sciences. The identification can be done by two ways, either by performing biochemical tests or by sequencing the bacterial genome. For this experiment I conducted various biochemical tests to identify the given unknown bacteria. The experimental unknowns were 7 different bacteria namely: Bacillus sp., Micrococcus luteus, Staphylococcus epidermis, Streptococcus lactis, Pseudomonas sp., Escherichia coli, Enterobacter aerogenes. Biochemical tests usually interact with the bacterial metabolites (chemical reactions done by the bacteria) to identify a specific bacterium or sometimes a specific genus of
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For the Oxidase test, first I took a substantial amount of bacterial goober using the loop from the given petri dish (this does not need to be from an isolated colony). I then placed this goober on the piece of filter paper and added a few drops of oxidase reagent to it. I observed for few seconds to see if there was any color change and wrote down my observation. After that, I obtained a F agar petri dish and streaked it with the unknown bacteria using a sterile loop. The petri dish was incubated for 24Hrs at 35oC. The petri dish was observed to see if there was any bacterial growth and once the whole dish was full of bacterial colonies, it was observed under an UV lamp to see if there was fluorescence or not. I decided not to do the IMViC tests because the first two tests concluded on what the unknown bacteria

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