Biofilm Research Paper

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RESEARCH PLAN Biofilm Inhibition of Chitosan/Hyptis suaveolens (L.) Poit Hydrogel Film on Staphylococcus aureus, Candida albicans and Pseudomnas aeruginosa A. RATIONALE Biofilm is a commonly found multicellular structure in bacteria where cells are attached to each other and embedded in an extracellular polymeric substance (Archer, Mazaitis, Costerton, Leid, Powers and Shirtliff, 2011) making it more resistant to antifungal drugs because of its complex structure and extracellular matrix (ECM) (Fanning and Mitchell, 2012). Also, presence of persister cells shuts down the microbial targets of the antibiotics that leads to chronic disease (Archer et al., 2011). Biofilm is the cause of resistance against antimicrobial agents and it protects cells…show more content…
To determine the antimicrobial and the biofilm inhibition of hydrogel films at 100:0, 95:5, 85:15, 75:25 and 50:50 solutions in Staphylococcus aureus, Candida albicans and Pseudomnas aeruginosa Questions What solvent may be used to extract Hyptis suaveolens (L.) Poit? How can the phytochemicals present in Hyptis suaveolens (L.) Poit be determined? How can the Nanochitosan powder be extracted from shrimp shells and be characterized in terms of molecular weight and crystalline structure? What will be the measurements of the physical and mechanical properties of the produced hydrogel films from Nanochitosan/Hyptis suaveolens (L.) Poit and Alginate/Hyptis suaveolens (L.) Poit in terms of in terms of thickness, transparency, tensile strength, swelling behavior and gel fraction? What will be the effects of the hydrogel films on the growth and biofilm formations of the Staphylococcus aureus, Candida albicans and Pseudomnas aeruginosa? Hypothesis 1. Methanol will be used as a solvent in distillation process and also soxhlet apparatus because of its low boiling temperature and it is non-polar which are necessary for choosing solvent in plant…show more content…
Poit may present bioactive compounds such as steroids, alkaloids, flavanoids, tannins and glycosides. Extraction of chitosan from shrimp shells will yield 34% with 89.79% degree of deacetylation and molecular weight of 1,599,588.029. Possible thickness of the produced hydrogel films can be between 59.7 µm to 75.3 µm. In addition hydrogel film may present quick absorption in first 30 minutes and slows down as the time passes by. Swelling behavior percentage ranges from 0%-350%. Weight loss of hydrogel film may range 87.8 ± 7.4% and 308.3 ± 11.8 %. Due to the presence of plant extract the expected inhibition for Staphylococcus aureus ranges from 4.8±0.2 - 14.3±0.30, Candida albicans 15.2±0.25 - 20.3±0.9 and Pseudomnas aeruginosa 15.2±0.25 -

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