Lab Report

3.0 MATERIAL AND METHOD
3.1 General techniques
3.1.1 Collection of sample Five sample was collected from Seri Manjung Mangrove (TSMa, TSMb, TSMc, ASMd, ASMe) and two samples from Batu Maung Mangrove area (BMa and BMb). The sample was collected inside a sterile falcon tube and transported immediately to Biology Lab at USM Biology School. Isolation procedure was immediately set in motion. The remaining sample was stored inside a 4oC refrigerator for further uses.

3.1.2 Weighing Weighing machine August Sauter Gmbn D-7470 Albstadtl-Ebigen was used to weigh every materials that was used in the experiment.

3.1.3 Optical Density Determination Optical density (OD) determination was carried out by using Shimadzu UV-120-01 with 540 nm wavelength.

3.1.4 Sterilization Techniques Materials were

The morphology of the colony on PDA such as the size of colony, margin of colony, color of colony and also the color of the agar were observed. The rate of growth was noted as well. In microscopic observation, a drop of Lactophenol Cotton Blue was put on a glass slide. With the sticky side facing the colony’s surface, a strip of clear cellophane was pressed on to the 7 days old fungal colonies surface using forceps until the hyphae of the fungal colonies was sticked on to the cellophane. The cellophane stripes was pulled gently and sticked on the prepared slides completely. The side of the cellophane was sealed with cutex to prevent dehydration. This method known as ‘‘Cellophane Tape Mount’’ suggested in the book manual ‘’Medically Important Fungi : A Guide To Identification’’ by Davise A.Larone. The slides was observed under microscope with magnification of 100x. The microscopic morphology of the fungi was compared to the book manual ‘’Illustrated genera of Imperfect Fungi’’ by H.L.Barnett to determine the genus of the fungi