The materials that we used when conducting this experiment are: dissecting microscope, 3 petri dishes, C. elegans (male, female, and hermaphrodites), worm pick tool, agar, Bunsen burner, Parafilm, incubator, and flint spark lighter. The methods of this experiment are really simple. When we started the experiment we all washed our hands and wore gloves. Each group member did their part to conduct and successful experiment, one group member plugs the Bunsen burner to the gas pipe and turned on the gas, then used flint spark lighter to set the flame on the Bunsen burner. While the second group member is setting the dissecting microscope and making sure the lenses are clean.
The Detector: The separated ions are then measured, and the results displayed on a chart. Mass spectrometry has both qualitative and quantitative uses. These include determining the structure of a compound, quantifying the amount of a compound in a sample and determining the isotopic composition of elements in a molecule. This technique basically studies the effect of ionizing energy on molecules. It depends upon chemical reactions in the gas phase in which sample molecules are consumed during the formation of ionic and neutral species.
Introduction: The objective for the lab was to determine properties of materials by using a tension test. The properties that were determined was Young’s Modulus, ultimate tensile strength, and yield strength. Three aluminum alloys were tested. The first was the AA 2024 , which is high strength used in aerospace applications. The second specimen was AA 5052, which had low strength, but high ductility.
GRAVIMETRIC DETERMINATION OF MOISTURE AND PHOSPHORUS IN FERTILIZER SAMPLES Magdato, K. C. , Balbuena, J. S. Institute of Chemistry, University of the Philippines, Diliman Quezon City Date Performed: August 27, 29 and September 3, 2014 Date Submitted: September 10, 2014 Abstract The experiment aims to use gravimetric method of analysis in determining the moisture and phosphorus content in a fertilizer sample which is important in plant growth. The procedure included constant weighing of the crucible and sample. The addition of ammonia and magnesium sulfate heptahydrate caused the formation of the precipitate identified as magnesium ammonium phosphate hexahydrate(MgNH4PO46H2O). The weight of the precipitate was used to determine the mass of
The sample will prepare by using the KBr pelleting method. The sample will mix with the KBr pellet in the mortar and pestle to grind until become fine powder form. The mixture will then press in a special die at 10,000 lb in-2 to yield a transparent disc. Before running the test of the sample, the background spectra will collected under identical conditions is subtracted from the sample spectra automatically. The spectrometer analysed the compound in the KBr disc form and a spectrum was displayed after the testing of the sample was completed.
4 Procedure Preparation of Test Solution The sample was prepared by adding 100 mg of powdered in 10 mL of methanol, soaked for 15 min. Centrifugation was done and the filtrates or supernatants were used as the sample solution. Out of this filtrate 50 μL of samples were dissolved in 1 mL of toluene and were used as the sample solution. Preparation of the standard solutions Wedelolactone, Ecliptaalbasaponin-I and Ecliptaalbasaponin-II were used as marker compounds; these markers were dissolved in methanol at a concentration of 1 mg/ml. The extracts of Bhrungaraj prepared were shaken and sonicated in methanol at a concentration of 10 mg/ml.
To minimize the variation of sampling, the tissue specimens were taken by two of the authors (XXXXX and XXXX). The tissue samples were collected as previously described.11, 12 All tissue specimens were immediately immersed in RNAlater® solution (Qiagen, Germany) and then stored at -20 °C until RNA extraction. RNA
2.0 Materials and Methods 2.1 Sample preparation Experiments are conducted on aluminum alloy with nominal composition of Si 0.4, Fe 0.5, Cu 2.0, Mn 0.3, Mg 2.9, Cr 0.28, Zn 6.1, Ti 0.2 and Al balance in wt%. Cylindrical specimens with 24 mm diameter and 5 mm thickness were used for the ENi-B deposition. Aluminum and its alloy exposed to air are always covered by a dense oxide coating that must be removed before the parts are to be plated; therefore to enhance adhesion proper pretreatment is obligatory. Sample preparation for coatings was as follows: (a) mechanically polished with wet 400, 600, 800 and 1000 grit SiC paper, (b) cleaning with acetone followed by a methanol wash, (c) acid pickling for 2min (10% HNO3 + 3% HF by volume), the samples
Subjects were excluded if they had injuries that could be made worse by performing the tests. Equipment used in this study included a stadiometer with a mechanical counter (Holtain Ltd. Crymych, Dyfed), a weighing scale (Sartorius ISI 10 AG Gottingen, Germany), an incline pull up bar, stepping boards and an IPhone application (Intervals). During the collection of bio data, the stadiometer is used for measuring the stature of the subjects and the weighing scale is used for measuring the weight of the subjects. The incline pull-up bar was for the subjects to grip and the stepping boards were for them to rest their heels on
.1 Soil sampling and bacterial isolation A total of 17 soil samples were collected from the rhizospheres of various crop plants at two sites located in Yangzhou city, Jiangsu province, China (See Table 1). The soil samples were collected from a depth of 1–15 cm, put in polythene bags, and transferred to the laboratory. One gram was taken from each soil sample and added to 10 ml of sterilized distilled water. Serial dilutions of this mixture, up to 10-7 were then prepared. Nutrient agar medium (peptone 10.0 g, beef extract 3.0 g, sodium chloride 5.0 g, agar 20.0 g per liter and final pH 7.0) was prepared and autoclaved at 121°C for 30 min.