Biosurfactant Essay

Short title: Functional Characterization of Biosurfactant Functional Characterization of Biomedical Potential of Biosurfactant Produced by Lactobacillus helveticus

1, 2Deepansh Sharma, 1*Baljeet Singh Saharan and 3Shailly Kapil
1Department of Microbiology, Kurukshetra University, Kurukshetra 136 119 INDIA
2Department of Biotechnology and Biosciences, Lovely Professional University, Jalandhar 3Dairy Microbiology Division, National Dairy Research Institute, Karnal132 001 INDIA
*[e-mail]

Abstract
The study aimed to investigating the functional properties of biosurfactant produced by Lactobacilli isolated from fermented foods. Biosurfactant waspartially purified by silica gel column chromatography and found as glycolipid. Biosurfactant

Dried biosurfactant was stored at 20 ºC for further experiments. Crude biosurfactant residues were partially purified in silica gel (60-120 mesh) column eluted with gradient of chloroform and methanol ranging from 20:1 to 2:1 (v/v).
Fourier Transmission Infra-red Spectroscopy (FTIR)
The functional groups of the purified Biosurfactants fromLactobacillus helveticus MRTL91was analyzed qualitatively by the FTIR method according to Kemp (1991).1 mg ml-1 concentration sample was prepared in chloroform.Afterwards, the20 µlsample was immediately put into the sample holder and FT-IR spectra were recorded. FT-IR scanning was conducted in ambient conditions. The resolution was set to 4 cm−1and the operating range was 500 to 4,000 cm−1. Sixty-four spectra per sample were recorded, averaged for each spectrum and ambient air as background.
Nuclear Magnetic Resonance (NMR) and Fatty Acid

It has been hypothetical that biosurfactant manipulate bacterial-surface interactions (Walencka et al. 2008). The biosurfactant showed antibiofilm activity against most of the pathogens but the spectrum of activity was varied for different microorganisms and also depends upon the concentration of the biosurfactant (Table 4).The highest antiadhesive property was observed against B. cereus (87 %), S. epidermidis (85 %), L. monocytogenes (84.3%), S. aureus (83.1 %), L. innocua (82.1 %), L. palantarum (75.1 %), L. bulgaricus (74.1 %), L. lactis (72.1 %),L. paracasei (70.5 %), L. lactis (69.1 %), L. delbruckii (69.2 %) on the other side, low activity was observedagainst E. coli (40.1 %), P. aeruginosa (39.1 %), S. typhi (36.1%), C. albicans (21%) and S. flexneri (20.1%) (Table-4). The antiadhesive activity against yeast C. albicans was quite low even at the highest biosurfactant concentration assayed i.e. 25 mg ml-1. In addition it is remarkable that, a high degree of antiadhesive activity was observed (from 69.2 to 87 %) with the highest biosurfactant concentration assayed (25 mg