Birby Bauer Disk Diffusion Report
The Kirby-Bauer disk diffusion method is utilized as a part of this trial so as to watch the viability of a sure anti-microbial. This strategy incorporates the spreading of the two sorts of bacteria being concentrated on an agar in a petri dish with the goal that they can produce. One agar contained Wild S. Aureus and another agar contained MRSA. Paper circles are let to absorb refined water, and equivalent centralizations of the accompanying antibiotic arrangements: Methicillin, Vancomycin and XR21347. At that point, these paper plates are put onto both agars and left as a base for bacterial development. For right and dependable perceptions, the plates ought to be marked by antibiotic arrangement present. This set-up ought to then be left
Ampicillin Lab Report
680 Words | 3 PagesIn this experiment, Tetracycline and Ampicillin were used to test for the antibiotic resistance of Pseudomonas fluorescens, lab-grown bacteria. The bacterial colonies showed resistance to Ampicillin; however they did not show resistance to Tetracycline. The results suggested that Tetracycline was much more effective in killing the bacterial growth, in comparison to Ampicillin. It was confirmed that a single dose of Tetracycline had killed the surrounding bacterial growth, as the bacterial colonies around where the drug had been placed, died and showed no growth. The bacterial plates also suggested that Ampicillin was ineffective in killing the bacteria, as zones of inhibition were not present, despite using two disks of this drug.
Water Bottled Water Lab Report
1372 Words | 6 PagesThe purpose of this experiment is to compare different types of water, including, well water, city water, bottled water, and water from a water fountain. I plan to take tap water from multiple faucets, with some using well water and others using city, use a brand of bottled water, and samples from a water fountain to grow bacteria. “Water is an excellent solvent and can be sourced from almost anywhere on Earth. This property makes it prone to all kinds
Disk Diffusion Test Lab Report
1130 Words | 5 PagesThe testing for affectability of a life form to antimicrobial agent is normally done utilizing agar dissemination or disk diffusion test. The parameters of this test were indicated (or institutionalized) by the researchers W. M. M. Kirby and A. W. Bauer and is likewise alluded to as the Kirby-Bauer antibiotic testing. In this technique, anti-toxins or antibiotic are impregnated on a specific extraordinary kind of paper circles and are put on the surface of agar containing the bacterium and parasitic (fungi) of our interest. This outcomes in the dispersion of antimicrobial agent into the surrounding medium. The diameter of the zone of inhibition will decide the adequacy or sensitivity of the antibiotic; the bigger the diameter, the more
Macconkey Agar Lab Report
266 Words | 2 PagesGram-Positive bacteria that are inhibited with MacConkey agar due to crystal violet and bile salt presence . “3. What ingredient(s) makes MacConkey agar differential?” MacConkey agar are inhibited by a pH indicator (neutral red), and a disaccharide ( Lactose) “4.
Cellulitis Research Paper
1133 Words | 5 PagesThe purpose of gram stain and culture is to guide antimicrobial therapy. The laboratory will typically test bacterial growth against commonly prescribed antibiotics and determine resistance patterns. Bacterial cultures generally take at least 2 days to
Picot Question
941 Words | 4 PagesHand washing or isolation of the sick persons with infections in the prevention of hospital acquired infections. 5. Does the use of hand washing, and antisepsis lower the rate of hospital acquired infections? The fifth PICOT question is selected because of the reported low compliance percentage among medical caregivers.
Bacterial Count Case Study
897 Words | 4 PagesTotal Bacterial Count Table 1 presents the total bacterial count of treatments at different time intervals. There are no significant differences between treatments before EM application, one day, two weeks and three weeks after EM application when analysis of variance (ANOVA) was tested on each time interval. (Appendix C Table C.1; Table C.2; Table C.3; Table C.4) Table 1. Total Bacterial Count of water from DMMMSU-SLUC Wells at different time intervals.
Summary Bonnie Bassler
671 Words | 3 PagesHowever, when these bacteria are grouped together to have high cell density, the molecules they secrete amount to a certain number, and once that number is reached, the behavior of that bacteria is switched on and in this case, bioluminescence is created. Similarly, in my project, I am screening the anti bacterial activity using oils. Before I use the oil, I have to culture the bacteria overnight so that I could use them in the plates after 16-18 hours of incubation. Based on the talk, I believe that I have an idea on how bacteria grow. This Ted Talk has inspired me about science in numerous aspects.
Osmosis And Diffusion Lab Report
388 Words | 2 PagesIsra Romani Lab Report 2: Diffusion and osmosis through non-living membranes Introduction Cells contain membranes that are selectively permeable which allow certain things to pass into and leave out of the cell. The process in which molecules of a substance move from an area of high concentration to areas of low concentration is called diffusion. Whereas Osmosis is the process in which water crosses membranes from regions of high water concentration to areas with low water concentration. While molecules in diffusion move down a concentration gradient, molecules during osmosis both move down a concentration. During the osmosis both move down a concentration as well as a cross it.
Simple Diffusion Lab Report
598 Words | 3 PagesOsmosis is a process where the solvent molecules pass through a semipermeable membrane from an area of high concentration to an area of low concentration until it reaches equilibrium. These solvent molecules, typically water, move freely. Carbon dioxide is able to move across the cell membrane through a process called simple diffusion. This works in a similar manner, carbon dioxide is able to move freely across the cell membrane from low to high concentration. When oxygen is breathed in, the red blood cells in the lungs have a low concentration of oxygen and a high concentration of carbon dioxide.
Essay On Staphylococcus Aureus
1041 Words | 5 PagesStaphylococcus aureus Introduction/ Background information Staphylococcus aureus (S. aureus) is yellow-pigmented colonies and this is a reason for (aureus = golden) in its genus name. S. aureus is a spherical shape (Coccus), gram positive bacterium that usually present in pairs as seen in microscope.it has short chains, or bunched, grape-like clusters, non-motile, no spores and facultative anaerobic. Members of this species can survive in the aerobic or anaerobic conditions and they can adapt to any of situations. The best environment for them to grow is in dilute salt concentrations and low moisture, which partially introduces human's nasal secretions and skin's surface to be a suitable place for this microorganism to grow. This bacterial growth can lead to staphylococcal infections in skin, nose and throat of human
Important Measures To Prevent The Spread Of Infection Research Paper
949 Words | 4 PagesThe pictures that were incorporated in each section give a visualization of what each section is about, and what to do in each situation; even a child or illiterate person can have an idea what the brochure is all about (www.jointcommission.org). This topic interests me because hand hygiene and infection control as a whole, have great importance in my daily life. Since health care-associated infections (HAIs) result from the delivery of health services in a health care facilities, as a nursing student this information will help me to be more aware to always maintain infection control when providing care to my patients to prevent the spread of infections (Potter, 2013).
Lb Agar Essay
863 Words | 4 Pagesıt is easy to prepare. It provides the environmental and nutritional conditions similar to their natural habitat. It is useful when a specimen contains a mixed bacterial colonies to achieve distinct colony-forming units. It also has disadvantages like it does not support the simultaneous growth of aerobic and anaerobic bacteria. Consequently, duplicate plates must be inoculated, and incubated in both atmospheres.
Rate Of Diffusion Lab Report
1137 Words | 5 PagesThe Effect of the Molecular Weight of a Substance to its Rate of Diffusion Paulo E. Garcia Group 2 Sec U-7L October 16, 2014 ABSTRACT The rate of diffusion was tested by using a petri dish with agar-water gel and three drops of different solutions. These solutions were observed for thirty minutes and measured every three minutes. The different solutions used are Potassium permanganate with a molecular weight of 158 g/mole diffused the greatest distance among the three, Potassium dichromate with a molecular weight of 294 g/mole diffused the lesser than that of Potassium permanganate, and Methylene Blue with a molecular weight of 374 g/mole diffused the least distance.
Lugdunin Case Study
1660 Words | 7 Pagesaureus bacteria did not develop resistance to lugdunin, even though with the exposure in people and in repeated exposure in the laboratory. In mice, S. aureus causes lugdunin applied topically was able to either partially or totally eradicate skin infections. This antibiotic cannot be used to clear S. aureus colonies in people recently. The researchers trust that the drug might be able to be taken systemically as it did not exhibit any signs of toxicity on a sample of human serum. Furthermore, lugdunin is not very soluble in water because it might be difficult for body to absorb it.
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