Table 1 mentions the Column volumes of Gel Filtration experiment: Void Volume (Vo), Elution Volume (Ve), Included Volume (Vi), Entire Volume (Vo + Vi), and Inert Volume (Vg). Void Volume (Vo) came out to be 1.25 ml. It was calculated by adding all the fractions that contained blue dextran. Blue Dextran was the biggest substance in size which made it impossible to fit in the available pore sizes of the beads. Void Volume represents the space between the beads in column. In addition, Elution Volume (Ve) came out to be 5.00 ml. It was calculated by adding all of the fractions from beginning to the last fraction that contained yellow food dye. Yellow food dye was the smallest substance in size which made it easy for it to fit in all the pore sizes …show more content…
In addition, substances collected in fractions were fairly separated because different colors were noticed as it is seen in Table 2: Gel Filtration Fractions. Fraction 4 contained light blue color which suggested the presence of Blue Dextran but in results it was concluded that Fraction 4 did not contain protein. Thus Fraction 5 which contained Dark Blue color probably was Blue Dextran. Fractions 6 and 7 were noticed with light brown color which represents the hemoglobin. Fractions 16-20 contained yellow color which suggests that they represented yellow food coloring dye. Since BSA is colorless substance and the results implied that fractions 5-10 had proteins present in them. Thus, BSA could probably be in Fractions 8, 9 and 10. SDS-P.A.G.E. results confirms that BSA was present in fraction 5, 6, 7, and 8 whereas the Hemoglobin was present in fractions 5, 6, 7, 8, 9, and 10. It is so because BSA is only slightly larger than Hemoglobin: Molecular weight for BSA is 68 kD whereas Hemoblogin is 64.5 kD. As mentioned in table 4, the molecular weight of Hemoglobin is 16kD but it is a tetramer so it has 4 subunits of 16 kD which actually makes it 64 kD. In addition, SDS – PAGE results reveals that the Blue Dextran was the largest substance which eluted first followed by BSA which was smaller than Blue Dextran
The sample was then incubated at 56°C to lyse the tissue. The sample was checked every fifteen minutes and vortexed between each checking for an hour and a half until the tissue was completely lysed. The tissue sample was then again vortexed. Next 200 microliters of buffer AL was added and
The presence of macromolecules is able to be detected in solutions such as glucose, sucrose, starch, and proteins as well as other common foods. These other common foods include oats, soda, gelatin, and apple juice. There are four classes of macromolecules such as monosaccharides, disaccharides, polysaccharides and proteins. Each of these can be found using different tests such as the Benedict’s test, the Iodine test, or the Biuret test. Although there is no specific test for disaccharides it can be determined if the original color has not changed.
Lab #1: Examining and Verifying Various Macromolecules’ Reactions for Categorization of the Tested Solutions Jae-eun Park 20608251 Lab Partners: Jasmine Harding-Bake, Karolane Blais TAs: Lilia Shabon, Ryan Rashidi BIOL 130L Section 015 Experiment performed on: September 21 2015 Monday 2:30PM – 5:20PM B2 151 INTRODUCTION Various macromolecules share similar characteristics due to their shared functional groups, and in this lab, this was examined and categorized through three tests, namely the iodine test, Benedict’s test, and the biuret test. Iodine test will show the presence of starch and glycogen of the tested material by the color change to dark blue and earthy red respectively from its normal lightly yellow color. This
INTRODUCTION CHROMATOGRAPHY Chromatography was originally developed in the year 1903 by the Russian botanist Michael Tswett in percolating a petroleum ether extract through a glass column packed with powered calcium carbonate for the separation of colored pigments. Elution means a chromatographic separation involves the placing of the sample into a liquid or solid stationary phase and passing a liquid or gaseous mobile phase through or over it. Whether the separation takes place on a planar surface or in a column according to these chromatographic techniques are classified.
In initial elution using 25 mM Tris/HCl pH 7.2 buffer test tube #10 was determined to have the highest peak with an absorbance of 0.12. In elution with 25 mM Tris/HCl pH 7.2 1.0 M salt buffer test tube #20 was determined to have the highest peak with an absorbance of 0.4. These peaks represent the amount of protein present within the fraction. The first peak may include proteins with many positive amino acid residues. The second peak contained proteins with negatively charged amino acid residues.
Table 1 Results DDA Concentration Initial Mass(g) Time Interval Recovered Mass Cumulative Mass (g) Cumulative Recovery (%) Ln[(Rinf -R)/ Rinf] R=Rinf(1-e-kt) (M) (g) 10^(-5) 160 0 0
The Gastrocnemius Muscle of Rana pipiens is an Appropriate Model for Skeletal Muscle Contractile Kinetics When Compared to Peer-Reviewed Models Georgia Institute of Technology BMED 3110: Quantitative Engineering Physiology Laboratory I Section B: Team Baboons 16 November 2014 ABSTRACT The dynamics of skeletal muscle kinetics can be quantified using various experimental methods involving stimulated muscle contractions.
They often have a golden or yellow colour and β–hemolysis on sheep blood agar (Wang, Braughton,
The objective of this study was to test the phototactic response of Daphnia when exposed to red (>600 nm) and white light. A 30 x 2 cm clear acrylic mesocosms with a 10 cm counting area was filled with distilled water and 10 Daphnia. We counted the number of Daphnia that traveled to the lit counting area after 10 minutes. There were twice as many Daphnia in the lit counting area for the control (white light) compared to the experimental group (red light). The results showed that red light had a negative effect on the phototaxis of Daphnia.
Oxidized dextran was reacted with sodium periodate to oxidize. Preparation of Oxidized dextran was done by dissolving dextran (10g) in 100mL of distilled water, then a desired amount of NaIO4. This solution was stirred at a room temperature and shielded from light for 6 hours. The oxidation was terminated by the addition of 2 ml f ethylene glycol. To get the final Odex, the resulting solution was exhaustively dialyzed against water for three days and lyophilized.
In Table 2, the initial mass of the weight buret in trial one was 15.23 grams, the final mass of the buret was 14.14 grams. The second trial’s initial buret mass was also 14.14 grams, because no solution was used in between trials; however, the final mass was 13.04 grams.
These substrates include: glucose, palmitate, amino acids and propionate. The aim was to make sure that any type of blood spot card would be able to show the chemical structure of the chemicals on them. Therefore, four different sampling cards were chosen: FTA DMKA-A, FTA DMPK-B, VWR 237 and Protein Saver 903. These cards supposedly have different structures, so it would be possible to see which one was more successful with each of the chemicals named above.
Chapter Vitamin B12 Absorption and Transport in Human Body Omar Abuyaman, MSc Department of Clinical Biochemistry, Aarhus University hospital, Aarhus, Denmark. Abstract Mammals are unable to synthesize B12. Instead, they have a sophisticated multistep pathway for specific and efficient transport of this vitamin from its food source to the target body cells.
The volume in the test tube was adjusted to 0.1 ml with appropriate buffer. Five milliliters of protein reagent was added to the test tube and the contents were mixed either by inversion or vortexing. The absorbance at 595 nm was measured after 2 min and before 1 hr in 3 ml cuvettes against a reagent blank prepared from 0.1 ml of the appropriate buffer and 5 ml of protein reagent. The weight of protein was plotted against the corresponding absorbance resulting in a standard curve used to determine the protein in unknown
It also better to ensure that the materials like hemocytometer and pipettes are sterilized and clean so that there would be lesser or no artifacts would be seen under the microscope. The researcher was to use trypan blue exclusion method to test for cell viability, observe the non-viable and viable cells, and was able to have a cell count using the